Search PubMed⌕ Search

Biomedical subjects

K Kubo

Publications and source records attributed to K Kubo.

At least 577 records · Page 32Linked to original sources
PubMed1989

Penicillin G-induced cutaneous anaphylaxis in the guinea pig.

Allergic cutaneous responses were induced by intradermal injection of penicillin G (PCG) and PCG-bovine serum albumin (BSA) conjugates onto the back of guinea pig actively immunized with PCG potassium (25 mg/animal) incorporated in Freund's complete adjuvant. The PCG-induced response was characterized macroscopically by erythema and edema with a maximum level at 24 hrs after elicitation and microscopically by the infiltration with basophils, macrophages and lymphocytes following with neutrophils. In addition, intensity of macrophage-infiltration shared a similar time course change with those of erythema and edema. These suggest that this response is associated to a delayed type hypersensitivity of Jones-Mote type. On the other hand, in the PCG-BSA-induced response the edema with erythema at the early phase was a noticeable observation and this response disappeared within 12 hrs, although the erythema continued by 24 hrs. Microscopically, the degranulation of mast cells and severe infiltration with neutrophils in the early phase and the infiltration of eosinophils in the late phase accompanying the infiltration with monocytes, basophils and lymphocytes were characteristic findings, which suggest that PCG-BSA response is a similar hypersensitivity to an atopic dermatitis. As mentioned above, we confirmed two types of allergic cutaneous responses in the guinea pig immunized with PCG.

Animals↗
PubMed1989

[Influence of residual plaque score during initial treatment on pocket reduction rate at individual tooth surfaces].

The purpose of this study is to examine the relation ship between residual plaque score at an individual tooth surface during initial treatment and the pocket reduction rate at the evaluation time. Thirty-nine adult periodontal patients (mean age 30.2 years) who O'Leary plaque control record (PCR) reached a level of 10% PCR, were selected for this study. A total of 5638 surfaces were monitored by recording probing pocket depths at the disto-buccal (a), mid-buccal (b), mesio-buccal (c), disto-lingual (d), mid-lingual and (e), mesio-lingual (f) regions, and by examining O'Leary plaque control records. The results were as follows: 1) The residual plaque score at all teeth surfaces was 14.74 +/- 19.21%. 2) The lowest plaque score were seen at 54/45, and 21/12. 3) The highest plaque score were seen at 7/7, and 76/67. 4) Well-responding sites that showed a high residual plaque score and a good pocket reduction rate, were 5/5 (initial probing pocket depth 3.0-3.5mm, a), and 4/4 (initial probing pocket depth 3.0-3.5 mm,f; 5.0-5.5 mm, f). 5) 7/7 (initial probing pocket depth 3.0 mm a, b, d), and 7/7 (initial probing pocket depth 3.0-3.5 mm, f) showed a high residual plaque score and a low pocket reduction rate. 6) The normal pocket response occurred at a level of residual plaque score and a low pocket reduction rate. 6) The normal pocket response occurred at a level of residual plaque score of less than 25%. In order to gain good pocket response, it is important to keep the level of residual plaque score at less than 25% rather than to keep the level of PCR at 10% or 20% (as several authors have previously stated). 7) A low residual plaque score during treatment is therefore more important than a low PCR.

Adult↗
PubMed1989

[Pocket reduction after initial treatment in relation to tooth type and site].

The purpose of this study was to compare differences in pocket reduction rate between different tooth types and sites after initial treatment. Forty-one adult periodontitis patients (mean age 40.8) whose O'Leary plaque records during initial treatment were maintained at a level of less than 10% (mean level 9.02 +/- 4.93%) were selected for this study. A total of 5983 surfaces were monitored by recording probing pocket depths (P.P.D.). The results obtained were as follows. 1. Significant probing pocket depth reduction was obtained following initial treatment (P less than 0.001). 2. Significantly improved teeth were 421/124 (p less than 0.001), 5/5 and 53/35 (p less than 0.05), and 32/23 (p less than 0.05). Deep residual P.P.D. were observed in 1/1, 6/6, 7/7, 6/6 and 7/7 (p less than 0.001), and 2/2 and 3/3 (p less than 0.05). 3. Response to treatment by tooth type was favorable in 54/45 and 431/134 and was poor in 71/17, 7/7. 4. Mandibular residual pocket depths were much deeper on proximal surfaces than on the mid-facial and lingual surfaces. Maxillary pockets on palatal surfaces were significant deeper than on facial ones. 5. Poor response to treatment was treatment was observed on the mesio-palatal surfaces (p less than 0.001), mid-palatal surfaces (p less than 0.01) of 6/6 (p less than 0.001), the distolingual surfaces of 7/7, and 7/7 (p less than 0.001), and the mesio-palatal surfaces of 1/1 (p less than 0.05). Favorable responses were observed on the disto-buccal and the id-palatal surface of 4/4 (p less than 0.05), mesio-distal buccal surfaces of 5/5 (p less than 0.05) and mesio-lingual surfaces of 4/4 (p less than 0.05). This results clearly indicate which tooth types and root surfaces must be paid careful attention to during treatment.

Humans↗
PubMed1989

Possible mechanism for the inhibition of gastric (H+ + K+)-adenosine triphosphatase by the proton pump inhibitor AG-1749.

Mechanisms related to the inhibition of (H+ + K+)-adenosine triphosphatase (ATPase) by 2-[[[3-methyl-4-(2,2,2-trifluoroethoxy)-2-pyridyl]methyl] sulfinyl]-1H-benzimidazole (AG-1749) were studied using canine gastric microsomes. AG-1749 (1-100 microM) inhibited the K+-stimulated ATP hydrolysis and vesicular accumulation of H+. AG-1749 bound to the microsomes concentration-dependently and decreased the number of free SH groups; the binding correlating with the enzyme inhibition. Both the binding and inhibition were antagonized by dithiothreitol. N-ethylmaleimide inhibited the (H+ + K+)-ATPase and decreased the binding of [14C]AG-1749 to the microsomes. The inhibitory effect of AG-1749 gradually increased with incubation time, and was enhanced by lowering the pH. AG-2000 and AG-1812, acid-induced rearrangement products of AG-1749, inhibited (H+ + K+)-ATPase potently, rapidly and independently of pH; the inhibition was antagonized by dithiothreitol. We propose that AG-1749 is transformed into its active forms within the acidic compartment of the parietal cells and that the active compounds inhibit (H+ + K+)-ATPase activity by reacting with the SH groups of the enzyme.

2-Pyridinylmethylsulfinylbenzimidazoles↗
PubMed1989

[Effectiveness, accuracy, and acceptability of breast self-examination as a breast cancer screening method].

(Breast self-examination) BSE was reviewed in terms of effectiveness, accuracy, and acceptability. Evidence on the effectiveness of BSE is inconsistent. Some researches show the effect of BSE for early detection and better prognosis of breast cancer, but others do not. Sensitivity and specificity of BSE has not been determined yet, but they are assumed to be lower than those of the clinical examination and mammography. Education of BSE for women consistently attained an improvement in frequency and skill of BSE practice. BSE may be a useful method for detecting breast cancer at the interval of periodic clinical examination.

Adult↗
PubMed1989

[Radiotherapy of squamous cell carcinomas of the tongue--a statistical review].

The cases of 36 patients referred for radiotherapy of the tongue after an excisional biopsy or after an inadequate excision of the tongue cancer have been reviewed. In 32 patients treated by radiotherapy, the actuarial survival rate at five years was 86%, and the local rate control, was 82%. The local control rate for those treated by interstitial radiotherapy (Int. RT) was successful in 12 out of 13 cases, and the rates for those treated by external radiotherapy (Ext. RT) followed by Int. RT, or by the intraoral cone technique with electron beams (Elec.), or by Ext. RT, or by Elec. followed by Int. RT, or by no additional therapy were 7 out of 9, 4 out of 5, 2 out of 4, 1 out of 1, and 2 out of 4, respectively. For such cases of cancer, subsequent interstitial radiotherapy is advisable to maintain local control.

Actuarial Analysis↗
PubMed1988

Cyclic AMP-dependent protein phosphorylation is involved in activation of the potassium current associated with endogenous cellular calcium in Euhadra neurons.

Intracellular injection of the catalytic subunit of cAMP-dependent protein kinase stimulated the outward potassium current associated with endogenous cellular Ca2+ which were abolished by either caffeine or cAMP-dependent protein kinase inhibitor. The principal action of this kinase in current activation may be to release calcium from the intracellular reservoir.

Animals↗
PubMed1988

Effect of recombinant human erythropoietin on the anemia of chronic renal failure.

Phase I and Phase II studies of recombinant human erythropoietin (rhEpo) were conducted in normal volunteers and in anemic patients with chronic renal failure on maintenance hemodialysis. Three hundred U/person of rhEpo was administered intravenously to healthy normal volunteers in the Phase I study, resulting in no subjective or objective changes. In the Phase II study, 66 patients with chronic renal failure on maintenance hemodialysis with less than 20% hematocrit values were treated with rhEpo in doses of 50 U/kg to 200 U/kg two or three times a week. Hematocrit values increased significantly during the 12 weeks, and the patients' conditions improved. Patients previously requiring blood transfusions became transfusion-independent during our study. There were no obvious side effects, thus indicating the safety and efficacy of rhEpo in the anemia of chronic renal failure.

Adult↗