Dysplastic nevi as precursors to hereditary melanoma.
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Adult↗
Biomedical subjects
K Kraemer
Publications and source records attributed to K Kraemer.
[The pressure decrease in a laminal flowing, regularly ramified pipe system with applications to the blood circulation of humans].
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Blood Circulation↗
The rapid identification of Chido and Rodgers antibodies using C4d-coated red blood cells.
A simple method for the recognition of anti-Ch and anti-Rg, using red blood cells coated in vitro with C4d, is described. Of 58 anti-Ch and 20 anti-Rg sera studied, all caused direct agglutination of C4d-coated red blood cells in tests incubated at room temperature. Moreover, selected sera were shown to react strongly (3+ to 4+) with C4d-coated red blood cells when tested by the indirect antiglobulin test without prior incubation at 37 C. Sera containing other high-titer, low avidity (HTLA) antibodies were either nonreactive with C4d-coated red blood cells, or reacted to the same degree with "non-C4d-coated" trypsin-treated red blood cells. Similar findings were obtained in tests with non-HTLA antibodies of assorted specificities, including a variety of alloantibodies to high-incidence red blood cell antigens. These findings indicate that the use of C4d-coated red blood cells in investigative immunohematology may aid in the rapid identification of Ch and Rg antibodies.
Antibodies↗
Characterization of the epitope recognized by a monoclonal antibody highly specific for blood group M antigen.
The mouse monoclonal antibody M2A1 of IgG1 class, which is highly specific for blood group M antigen, was obtained and characterized by means of hemagglutination, enzyme-linked immunosorbent assay, immunoblotting, and inhibition assays. The use of modified M glycoprotein preparations for inhibition tests and of variant McN and Henshaw red cell membranes for immunoblotting showed that M2A1 recognized an epitope including the NH2-terminal serine and sialic acid residues of glycophorin A, whereas the fifth glycine residue was not involved. The reactivity of the antibody with M antigen was distinctly dependent on ionic strength and pH; the optimum was at pH 8 to 9. The alpha-amino group of terminal serine residue was not necessary for the reaction with M2A1 antibody, and the results obtained suggested that the positive charge of this group contributed to decreasing antigen-antibody reactions at pH below 8. The reaction of the antibody with blood group N antigen was not detectable in any of the assays used.
Amino Acids↗