Search PubMed⌕ Search

Biomedical subjects

K Konoe

Publications and source records attributed to K Konoe.

6 recordsLinked to original sources
PubMed1992

Diagnostic usefulness of dipeptidyl aminopeptidase IV monoclonal antibody in paraffin-embedded thyroid follicular tumours.

Monoclonal antibodies to dipeptidyl aminopeptidase IV (DAP IV, EC 3.4.14.5) were raised and selectively applied to paraffin-embedded sections of thyroid carcinoma. Five monoclonal antibodies were found to stain paraffin sections of thyroid carcinomas. Using one of these antibodies (44-4), we studied retrospectively aberrant expression of DAP IV in thyroid carcinoma to determine whether immunohistochemical staining with DAP IV antibody is useful in pathological diagnosis. In almost all cases of thyroid follicular and papillary carcinoma, tumour cells were positive (99.0 per cent) with DAP IV, whereas the cases of follicular adenoma showed a low incidence (27.1 per cent) of positive staining. Follicular adenoma with incomplete capsular invasion had a higher positive incidence (50 per cent) than follicular adenoma without incomplete capsular invasion (9.6 per cent). In positive staining cases previously diagnosed as benign tumours, 11 benign cases reacting positively with DAP IV were rediagnosed as carcinoma after re-examination of more thyroid paraffin block sections or serial sections. These findings suggest that DAP IV monoclonal antibody is very useful in distinguishing thyroid follicular carcinoma from follicular adenoma.

Adenoma↗
PubMed1992

Immunohistochemical localization of dipeptidyl aminopeptidase IV in thyroid papillary carcinoma.

The localization of dipeptidyl aminopeptidase IV expressed aberrantly in thyroid carcinoma was studied by immunoelectron microscopy using a monoclonal antibody to the enzyme with special reference to enzyme-histochemical staining of the enzyme. Five thyroid papillary carcinomas were investigated including two lymph-node metastases. All cases showed the dense immunoreaction product on the apical membrane and only traces of the product on lateral membranes, endoplasmic reticulum and nuclear membranes. In one case only, the dense product was observed on basal tubular structures. Analysis, using immunogold labelling on pre-embedded cryosections, revealed that dipeptidyl aminopeptidase IV was localized on the luminal surface of cancer cells. Two different distribution patterns of dipeptidyl aminopeptidase IV activity staining, diffuse and apical patterns, reported previously were thought to be due to different amounts of dipeptidyl aminopeptidase IV in the cytoplasm of cancer cells. This enzyme-histochemical staining method is useful for pathological diagnosis of thyroid tumours and can be applied to clinical materials. The enzyme localization is revealed by the staining pattern.

Adolescent↗
PubMed1991

Dipeptidyl aminopeptidase IV staining of cytologic preparations to distinguish benign from malignant thyroid diseases.

Staining for dipeptidyl aminopeptidase IV (DAP IV [EC: 3.4.14.5]) activity was applied to aspiration biopsy specimens or imprint preparations of surgical biopsy specimens from thyroid tumors. Material was obtained from 55 patients with histologically proven thyroid diseases: 9 with papillary carcinoma, 5 with follicular carcinoma, 11 with follicular adenoma, 13 with adenomatous goiter, 8 with Hashimoto's thyroiditis, and 9 with other benign conditions. Most tumor cells, follicular lumina in cell clusters, and intranuclear inclusions were strongly positive for DAP IV in all examples of papillary or follicular carcinoma. In contrast, only a few epithelial cells were labeled for DAP IV in follicular adenoma and adenomatous goiter. Some Hürthle cells in Hashimoto's thyroiditis also were positive for DAP IV. When a DAP IV scoring system based on the percentage of positive cells and staining intensity was used, all benign tissues except one (from a follicular adenoma) were found to have extremely low scores. These results indicate that staining for DAP IV activity is a simple but useful tool to aid in distinguishing benign from malignant thyroid neoplasms.

Adenocarcinoma↗
PubMed1989

[Heterotransplantability of human anaplastic thyroid carcinoma line (HOTHC) in nude mice, and biological properties of the heterograft].

The heterotransplantability of HOTHC line (human anaplastic thyroid carcinoma) into the subcutis of BALB/c nude mice and the biological properties of grafts were discussed. (1) The HOTHC line showed high transplantability, and 1 x 10(4) cells produced anaplastic carcinoma (giant cell type) containing the colloid-like substance. (2) The grafted tumors grew rapidly and the mice were dead within 2 months after transplantation. (3) The number of leukocytes (neutrophils) of mouse peripheral blood increased as the tumor size increased, and the leukocyte count returned to a normal value after removal of the tumor. (4) The conditioned media of HOTHC line formed colonies of granulocytes (neutrophils) on soft agar. These phenomena revealed that HOTHC is a granulocyte-colony stimulating factor-producing line. (5) The conditioned media of HOTHC line showed promoting-effect on neovascularization on the chorioallantoic membrane.

Aged↗
PubMed1985

Establishment and characterization of a new clonal strain derived from human benign gastric teratoma.

Various kinds of cell-types such as squamous epithelial cells, columnar epithelial cells, sarcomatous cells and fibroblast-like cells proliferated in vitro during the initial period of the primary culture from the human benign gastric tridermal teratoma. The fibroblast-like cells designated HOGT clonal strain were established from a single cell of primary cultures. The HOGT clonal strain grew well and 70 serial passages were successively carried out within 17 months. Although the HOGT cells are karyologically normal, when transplanted into the subcutis of nude mice they produced a benign tridermal teratoma. The results obtained from cell culture and heterotransplantation of teratoma strain enabled us to hypothesize that the graft (tridermal teratoma) in mice may arise from a pluripotent stem cell.

Animals↗