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K Kohriyama

Publications and source records attributed to K Kohriyama.

15 recordsLinked to original sources
PubMed1992

[The suppression of CK activity in the brain by acrylamide].

In vitro, both acrylamide and bis-acrylamide inhibited the activity of purified creatine kinase (CK) from the human brain with almost the same potency. The inhibition was dose-dependent and non-competitive. In vivo, however, only acrylamide caused the splay of hind limbs and an awkward gait in rats, and inhibited CK activity in the brain. The CK activity of the regions of the brain investigated differed, and these distributions were well matched with those of human cases. The inhibition became more marked as the administration of acrylamide was repeated. While the distribution of 14C in the different regions of the brain did not differ after the injection of [14C] acrylamide, the degree of the suppression of CK activity differed in these regions and was most marked in the hypothalamus. Fifty-two-week-old rats appeared more susceptible to acrylamide than 8-week-old rats. However, no difference in the inhibition of the CK activity in the brain was noted between the older and the young ones. The possible relation of the regional differences of CK suppression with the pathogenesis of acrylamide encephalopathy is discussed.

Acrylamide↗
PubMed1992

[Abnormalities of coagulation, fibrinolysis and kallikrein-kinin system in 2 cases with remarkable eosinophilia].

We evaluated an initial activation of hemostasis (coagulation, fibrinolysis and kallikrein-kinin system) in two remarkable eosinophilia cases. The first case was a 29-year-old male who complained of a high fever, general exanthema and numbness of the limbs. He had peripheral blood eosinophilia (61.075/microliters) unknown etiology, and hypereosinophilic syndrome (HES) was diagnosed. The second case was a 60-year-old female who complained of numbness in her lower limbs. She also presented eosinophilia (10,379/microliters), and had clinical features of allergic granulomatous angitis (AGA). The administration of prednisolone led to clinical remission in both cases, including rapid improvement of eosinophilia, normalization of hypercoagulability and correction of platelet dysfunction. In hemostatic parameters, the titer of the soluble fibrin monomer complex (SFMC) became negative, and each value of FDP D-dimer, fpB beta 15-42, thrombin-antithrombin III complex (TAT) decreased, whereas the value of prekallikrein increased. A decrease in platelet aggregation with ADP, collagen and epinephrine was normalized by prednisolone administration. Hemostatic data in both cases indicate a possible relationship between the initial activation of hemostasis and the eosinophils, as well as the possibility of general and/or organic inflammations and intravascular thrombi formations in eosinophilia such as HES and AGA.

Adult↗
PubMed1991

Suppression of neurotoxicity of ammonia by L-carnitine.

Administration of ammonium acetate to mice caused seizures and alterations of brain energy metabolites. Pretreatment of animals with L-carnitine suppressed the frequency of the seizures and prolonged the latency to the first fit. When examined using the 'freeze clamp' method, brain energy metabolites were well preserved and the elevation of ammonia was less marked on administration of L-carnitine. Thus, L-carnitine suppresses ammonia-induced seizures and biochemical alterations of the brain in mice.

Ammonia↗
PubMed1991

Determination of atropine in biological specimens by high-performance liquid chromatography.

A sensitive and selective method for the determination of atropine in biological specimens has been developed. Samples alkalinized with sodium hydroxide were extracted with dichloromethane, and the organic phase was evaporated in a water-bath at 50 degrees C for ca. 10 min. The residue was dissolved in the mobile phase and injected into a reversed-phase column (TSK gel ODS-120A). The retention time for atropine could be varied by changing either the acetonitrile-water ratio in the mobile phase or the pH of the mobile phase. Acetonitrile-water (2:8, v/v) containing 6 mM phosphoric acid was used as mobile phase. Samples of 200 microliters or less were injected into the chromatography and measured at 215 nm. The recoveries of atropine added to drug-free specimens were satisfactory with coefficients of variation of 4% or less. Ninety-two compounds tested did not interfere with the assay of atropine. The method has been applied for monitoring atropine concentrations in cases of organophosphate and drug poisoning.

Adult↗
PubMed1990

[Effects of ethylene glycol on hepatic microsomal cytochrome P-450].

The effect of ethylene glycol on rat hepatic microsomal cytochrome P-450 was studied in vitro and in vivo. The destruction of cytochrome P-450 was not seen in vitro. The addition of 1 mM NADPH also did not change. When ethylene glycol was added to drinking water at a concentration of 1.0% for 7 days, there was no change in the contents of microsomal protein, cytochrome P-450, b5 and heme. While NADPH-cytochrome C reductase activity of the exposed group did not change, NADH-ferricyanide reductase activity increased significantly.

Animals↗
PubMed1990

[Effects of sexual difference on the toxicity of ethylene oxide. II. Glutathione metabolism and lipid peroxidation in the liver].

Wistar male and female rats were exposed to ethylene oxide (EO) at a concentration of 250 ppm, 6 hours a day, 5 days a week for 17 weeks simultaneously, and the effects of EO on the glutathione metabolism and lipid peroxidation in the liver in regards to sexual difference were studied. Although the liver weight of the male exposed group did not alter, that of the female exposed group increased when compared with the control group. This increase was not accompanied with the alteration of protein content per gram of liver in the cytosol fraction and total homogenate. Among the glutathione related enzymes in the liver, the glutathione reductase activity of both male and female exposed groups decreased compared with each control group, and there was no difference in the degree of the decrease. The glutathione peroxidase activity increased only in the male exposed group. The glutathione-S-transferase activity of both male and female exposed groups increased significantly. In the male exposed group, the activity increased greater than that in the female exposed group. In the male exposed group, the lipid peroxide level in the liver increased slightly but not significantly.

Animals↗
PubMed1990

[Delayed Sumithion intoxication].

A case of delayed Sumithion (fenitrothion) intoxication is reported. A 52-year-old man ingested 10 ml of Sumithion in order to commit suicide with alcohol and triazoram. Several hours later, he was admitted to our hospital because of clouding consciousness. On admission, he was somnolent, but had no other symptoms, especially suggested organophosphorus intoxication. After 40 hours, fasciculation and salivation, which are early symptoms of organophosphorus intoxication, gradually appeared. The concentration of Sumithion in the blood was measured during the course and its metabolism was represented phalmacokineticaly by a 2-compartment model. The retarded metabolism of the Sumithion was suggested by this model. It is considered that the retarded metabolism of Sumithion caused the delayed intoxication.

Biotransformation↗
PubMed1990

Brain metastasis from transitional cell carcinoma of the bladder--case report.

A rare case of brain metastasis from a transitional cell carcinoma (TCC) of the bladder is presented. A 66-year-old female underwent total removal of a bladder carcinoma and postoperative radiation therapy and chemotherapy. Right hemiparesis and seizures developed about 16 months later, and a solitary brain metastasis was discovered in the left Rolandic area. The tumor was totally removed and microscopic examination showed it to be TCC.

Aged↗
PubMed1990

[Breast cancer and B cell malignant lymphoma associated with Sjögren'ssyndrome--a case report and review of literature in Japan].

It is well known that some patients with Sjögren's syndrome develop lymphoproliferative disorders. Approximately 60 cases of Sjögren's syndrome associated with malignant lymphoma, predominantly of B cell origin, have been reported in Japan. On the other hand, fourteen cases of non lymphoid malignant neoplasm, including six of thyroid cancer and four of gastric cancer, have been recently presented in Japanese literature. However, cases of double malignant neoplasms in Sjögren's syndrome are very rare. We reported a case of Sjögren's syndrome associated with breast cancer and B cell malignant lymphoma in the paper. A 53-year-old female was seen with eye pain, xerostomia, finger pain and right parotid swelling in January, 1985. Keratoconjunctivitis sicca was found. She had had eye pain and Raynaud's symptom during the past seven years. Sialectasia of grade III by Rubin and Holtz was seen in sialography. Schirmer's test was positive. Pathological finding of sialadenitis in minor salivary gland confirmed diagnosis of Sjögren's syndrome. Laboratory findings showed leukocytopenia and polyclonal hypergammaglobulinemia. Rheumatoid factor and antinuclear antibodies including SS-A antibodies were positive. Tuberculin test was negative. Ratio of CD 4 per CD 8 T lymphocytes was increased in peripheral blood. She had history of breast cancer. Radical mastectomy, followed by irradiation was performed in 1980. Arthralgia and parotid enlargement were transiently diminished by administration of small amount of prednisolone. She was readmitted because of progressive bilateral parotid swelling in April 1988. Pathological finding of the parotid was consistent with malignant lymphoma of diffuse, small cell type (lymphoplasmacytic). Immunoperoxidase staining revealed positive cytoplasmic IgM, k in lymphoma cells.(ABSTRACT TRUNCATED AT 250 WORDS)

Breast Neoplasms↗
PubMed1990

[A case of temporal arteritis associated with polymyalgia rheumatica and subclinical Sjögren's syndrome].

A case of temporal arteritis (TA) associated with polymyalgia rheumatica (PMR) and subclinical Sjögren's syndrome (sub SjS) was presented in this paper. A 76 year-old-male was admitted with headache, fever and weight loss in April 1987. Myalgia of upper extremities and of thighs developed during the past two months before admission. He also had noticed bilateral wrist pain. Physical examination revealed slight cord-like thickening of left temporal artery with tenderness. Cerebral angiography disclosed narrowing of frontal and parietal branches of left temporal artery. Temporal artery biopsy was consistent with TA. Diagnosis of PMR was made by Bird's diagnostic criteria. Although sicca symptoms were not seen, sialography revealed moderate sialectasis. Pathological finding of salivary gland was compatible with sub SjS. Possibility of occult lymphoma was eliminated by CT scanning or myelogram. Laboratory evaluation disclosed slight anemia, leukocytosis and thrombocytosis. Blood chemistry showed no abnormal finding except for hyperfibrinogenemia. Serological studies indicated positive C-reactive protein and slight elevation of alpha 2 and beta globulin fractions of serum protein. Either rheumatoid factors, antinuclear antibodies, anti-smooth muscle antibodies, cryoglobulin or circulating immune complexes were not detected. The HLA-B8 and DR3, frequently detected in TA and SjS, were not identified. Tuberculin test was negative. These results suggested that immunological aberration not caused by genetic factors but by senescence would induce presence of TA associated with both PMR and sub SjS.

Aged↗
PubMed1989

[Optic neuropathy induced by thinner sniffing].

A case of optic neuropathy induced by thinner sniffing is reported. A 17-year-old girl, who had been sniffing a lacquer thinner for three months, suffered acute blindness followed by optic atrophy. Brain computed tomography revealed symmetrical low attenuation areas in the bilateral putamen. An analysis of the thinner by gas chromatography showed that its major components in a vaporized state were methyl alcohol and metyhl acetate. The optic neuropathy was induced by these solvents. In the diagnosis of the intoxication of mixed organic solvents, the measurement of each solvent in its vapor phase is considered to be quite important.

Acetates↗
PubMed1989

[Three cases of pernicious anemia complicated with chronic thyroiditis--the evaluations of immunological abnormalities].

We reported 3 patients with pernicious anemia associated with chronic thyroiditis and evaluated their immunological abnormalities in this paper. Two patients were females and another was male. They were all advanced in age. Levels of gammaglobulin including IgG was elevated in patients' sera. Values of complement components were within normal ranges. Although organ specific autoantibodies against intrinsic factors, parietal cells and thyroidal tissue antigens were detected, organ non-specific autoantibodies such as anti-DNA and/or anti-ENA antibodies were negative in the patients' sera. Percentage of T cells in peripheral blood remained within normal ranges. However, ratio of helper T/suppressor T cells was reduced considering patients' age. Moreover, Mantoux tests were negative in all of three patients. These results suggested that impaired cellular immunity, particularly imbalance of T cell subsets, caused by senescence, simultaneously induced pernicious anemia and chronic thyroiditis in these patients.

Aged↗
PubMed1986

Agammaglobulinemia in a pregnant woman.

A case of common variable immunodeficiency in a pregnant woman is presented. Lobar pneumonia developed in the sixth month of the pregnancy and her serum immunoglobulin levels were found to be extremely low. She was treated successfully with immune human serum globulin and antibiotics. She delivered a full-term baby without any troubles. Various immunologic studies were done in the peripheral blood of the patient and the neonate. Consequently, functional abnormalities of helper T cells were considered to be responsible for the hypogammaglobulinemia.

Adult↗