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Biomedical subjects

K Kikuchi

Publications and source records attributed to K Kikuchi.

At least 1,297 records · Page 72Linked to original sources
PubMed1981

Immunohistological detection of B lymphocytes in the rat lymphoid organs by using anti-rat B lymphocyte serum.

Anti-rat B lymphocyte serum (ARBS) was prepared by immunizing rabbits with purified B cells from rat spleen and by absorbing the rabbit serum with rat red blood cells and syngeneic sarcoma cells. The specificity of ARBS for rat B cells was confirmed by various cytologic and immunofluorescence studies. By indirect immunofluorescence on tissue sections of rat spleen and lymph nodes, anatomical localizations of B cells in lymphoid organs were lymphoid follicles of the lymph node and lymphocyte corona surrounding periarteriolar lymphoid sheaths of the spleen; while cells located in the thymus-dependent area were essentially devoid of immunofluorescence or no cytoplasmic immunofluorescence was observed in plasma cells. Thus, the B cell distribution in tissue sections was clearly demonstrated with ARBS. When the cross-reactivity of ARBS with mouse B cells was studied by complement-dependent cytotoxicity and immunofluorescence, it was found that mouse B cells shared at least one determinant of rat B lymphocyte specific antigens (RBLA) with rat B cells, and that the distribution pattern of the cross-reacting antigens in mouse lymphoid tissues was essentially the same as that of RBLA in rat lymphoid organs.

Animals↗
PubMed1981

The coexistence of gastric cancer and duodenal ulcers.

The records of 356 gastric carcinoma patients who were treated surgically were reviewed. Among them, six patients (1.7%) had duodenal ulcers. Five cancers of the six patients were "early" gastric cancers classified as type "IIc" or "IIc + III" according to "The general rules for the gastric cancer study in surgery and pathology". The cancers of the six patients were located in the lower half of the stomach. Gastric secretion activity was normal in four and above normal in two cases. Three had signet ring cell carcinomas, two had poorly differentiated adenocarcinomas and one had a well differentiated adinocarcinoma. Our results were similar to other results reported in many papers. In Japan gastric carcinomas coexisting with duodenal ulcers were more often confirmed in the "early" gastric cancer stage. This is probably because endoscopic examinations of the stomach were more often performed in Japan than in other countries because of te large number of gastric disease patients. In addition, it is assumed that the growth of gastric cancer in the stomach is slow because of the very active gastric secretion.

Adenocarcinoma↗
PubMed1981

Occurrence of two distinct subpopulations of suppressor cells in rats bearing chemically induced tumors.

Spleen cells from rats bearing syngeneic methylcholanthrene-induced sarcomas showed impaired proliferative responses to phytohemagglutinin (PHA) as well as to the KCl tumor extract. Studies were then performed to define suppressor cell systems possibly operating in tumor-bearing rats. For this purpose, thymus and spleen cells from tumor-bearing rats were admixed with tumor-immune or normal rat spleen cells and were assayed for their suppressing activity on proliferative responses of immune spleen cells to the tumor antigens and on those of normal spleen cells to PHA. Anti-rat T cell serum and glass-adherent technique were utilized to characterize the cellular source of suppressor activity in the thymuses and spleens of tumor-bearing rats. The results indicate that there are two distinct subpopulations of suppressor cells in tumor-bearing rats. The first is glass-adherent non-T suppressor cells that inhibit lymphocyte proliferative responses both to the tumor antigens and to PHA. The second is suppressor T cells and can only impair proliferative responses of tumor-immune lymphocytes to the tumor antigens. The former cells were found in tumor-bearers' spleens, while the latter cells were present in their thymuses and spleens. It is suggested from these data that complex mechanisms of immunosuppression are working in tumor-bearing hosts, one of which can be attributed to the existing suppressor cells in those host lymphoid tissues.

Animals↗
PubMed1981

[Clinical studies on cefoperazone treatment for bile duct infections (author's transl)].

The clinical effects cefoperazone (CPZ) treatment for 10 patients with bile duct infection were investigated and the following results were obtained. 1. Absorption and excretion of CPZ: CPZ of 500 mg was administered with intravenous one shot injection to 2 patients of which one was malignant tumor case and the remaining was non tumor case. A peak of CPZ level in serum was 63.0 mcg/ml and 54.7 mcg/ml respectively. CPZ level in bile was maximum at 81.5 mcg/ml and 292.5 mcg/ml respectively. 2. Clinical effectiveness: Of 10 patients treated with CPZ, the results were excellent in 2 cases, good in 5 cases and poor in 3 cases. 3. Side effects of CPZ: No significant side effects were observed in these patients except for one case in which rash appeared in whole body.

Adult↗
PubMed1981

Immunochemical characterization of human TL-like (T48) and Ly 1-like (T72) glycoproteins using two-dimensional polyacrylamide gel electrophoresis.

Xenoantisera, designated AT48 and AT72, were developed by immunizing rabbits with human thymus cell membrane and guinea-pigs with a T-cell glycoprotein purified from leukaemic T-cell membrane. Whereas AT48, after appropriate absorption, reacted exclusively with the majority of thymocytes (mainly cortical thymocytes) among normal lymphoid populations, AT72 reacted with virtually all of the thymus and T cells but not with B cells. Thymocytes, which were strongly reactive with AT72, existed in the thymic medulla, but cortical cells were also very weakly reactive with AT72. When cultured T-cell lines, all of which were derived from patients with T-cell-type acute lymphatic leukaemias, were tested for their reactivities with AT48 and AT72 by immunofluorescence, we found that AT48 stained certain T-cell lines, whereas AT72 stained all of the T-cell lines tested so far. Immunochemical data showed that AT48 precipitated a 48K molecular weight (mol. wt) glycoprotein from 125I-labelled thymus cell surface glycoproteins, which appeared to be very weakly associated with a 12K mol. wt component. These 48K and 12K mol. wt components precipitated by AT48 showed almost identical isoelectric points (pI) to those of HLA heavy chain and beta 2-microglobulin respectively. AT72, on the other hand, precipitated a 72K mol. wt glycoprotein from thymus and T cells as well as from leukaemic T cells. A less prominent 65K mol. wt glycoprotein was also precipitated by AT72 from thymus and T cells but not from leukaemic T cells. These two components showed almost identical pI ranging approximately from 4 to 7, and this marked charge heterogeneity observed was reduced by neuraminidase treatment, suggesting that it reflects the heterogeneity in sialylation of this molecular species. We concluded from these data that AT48 and AT72 used in this work could detect human homologues of mouse TL and Ly 1 antigens respectively.

Antigens, Differentiation, T-Lymphocyte↗
PubMed1981

Isolation and partial characterization of a 72,000-dalton glycoprotein (Tgp72) on human thymus and T cells: possible relationship to mouse Ly-1 antigens.

A xenoantiserum was raised in guinea pigs to a human T cell antigen (Tgp72) partially purified from cell membrane of T cell leukemia cells from a patient with chronic lymphosarcoma cell leukemia of T cell-type by using sequential chromatographic procedures. The antiserum reacted with all of human thymus and peripheral T cells as well as with a variety of cultured leukemic T cell lines, but not with B cells, granulocytes, and other cultured lymphoid cell lines, including normal B cell, Burkitt, myeloid, and non-T non-B cell lines. Immunoprecipitated material from 125I-labeled thymocyte, T cell, and T cell leukemia cell-surface glycoproteins by this antiserum was analyzed by SDS-polyacrylamide gel electrophoresis, which disclosed a single component with a m.w. of 72K (Tgp72). Further immunochemical data suggest that Tgp72 antigen on human thymus and T cells is an acidic glycoprotein composed of a single polypeptide rather than sulfhydryl-linked subunits, showing a marked electric charge heterogeneity in DEAE anion-exchange resin chromatography. This is further confirmed by the data obtained from 2-dimensional polyacrylamide gel electrophoresis, indicating that Tgp72 is a 72K m.w. glycoprotein with an isoelectric point ranging from 4 to 7. Because of its similarity in tissue distribution and immunochemical characteristics, Tgp72 on human thymus and T cells was assumed to be a human homologue of Ly-1 antigens in murine system.

Animals↗
PubMed1981

The effect of test meals on the PED test.

The effects of test meals on the PFD (pancreatic function diagnostant) test, which was introduced as "the B.T.PABA test" in the previous reports, were studied. The higher the protein in the test meal, the lower the urinary excretion value of p-aminobenzoic acid (PABA) and the first 3-h of PABA excretion. By using a test meal containing protein, the differences in digestion-absorption disorders between gastrectomy procedures (Billroth I and Billroth II) were clarified. Therefore, a test meal which contains a suitable amount of protein should be used in the PFD test to improve diagnostic accuracy.

4-Aminobenzoic Acid↗
PubMed1981

Hematemesis and Melena: gastritis.

The records of a total of 60 gastritis patients who showed hematemesis and/or melena are reviewed. The mean age was 40. The ratio of males to females was two to one. During the examination, 19 upper G-I lesions were confirmed in addition to gastritis. Sixteen patients had severe underlying diseases which were thought to be the cause of the bleeding tendency. Thirteen patients took alcohol or drugs which induced bleeding. Prognoses of the patients were relatively good when they were treated medically.

Adolescent↗
PubMed1981

The PFD test for pancreatic disease patients.

The PFD test was performed on various pancreatic disease patients. The urinary PABA excretion rate was significantly lower in chronic pancreatitis patients and in pancreatic carcinoma patients than in the controls, but no difference was observed between PABA excretion in the two diseases. After surgery, PABA excretion values of pancreatic carcinoma patients were significantly lower than those of non-pancreatitis non-pancreatic carcinoma patients. This was thought to be caused by the difference in residual anastomosed pancreatic tissues. Mild to severe pancreatitis, fibrosis and/or fat necrosis were seen in the pancreatic tissues of carcinoma patients, but these changes were minimal in non-pancreatitis non-pancreatic carcinoma patients. The PFD test is useful in detecting decreases in pancreatic exocrine functions or measuring the grade of pancreatic disorders in various pancreatic diseases, although it can not be used to differentiate pancreatic diseases.

4-Aminobenzoic Acid↗