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K Kikuchi

Publications and source records attributed to K Kikuchi.

At least 1,117 records · Page 62Linked to original sources
PubMed1986

Surface marker expression of human B-cell lymphomas.

We have generated a battery of monoclonal antibodies (MAbs) that distinguish different antigen molecules present in human B cells. Two antigen systems, termed L26 and L27, respectively, were expressed in most surface immunoglobulin-positive B cells and thus showed pan-B cell specificity. Two other antigens (L22 and L30) appeared to be expressed mainly on small resting B cells but not on large activated B cells. In contrast with L22 and L30, L29 and L4, the latter of which corresponds to OKT10, were not or little expressed on resting B cells, but were found on these B cells after activation either in-vivo or in-vitro with staphylococcal protein-A (SAC) plus interleukin-2 (IL-2) or with pokeweed mitogen (PWM). We also produced MAb (L10) that detected IL-2 receptors (IL-2R) consistently expressed on in-vitro activated B cells as described above. In contrast with L4, L10 and L29, L30 was lost from in-vitro-activated B cells, following the appearance of either L10 or L29 on these in-vitro-activated B cells. Then, we analyzed antigen profiles of B cell tumors using these MAbs as described above. Common acute lymphatic leukemia (CALL) expressed L4 and L30. B cell type chronic leukemia (B-CLL) and hairy cell leukemia (HCL) possessed most of the B cell antigens as described herein, and furthermore, HCL expressed IL-2R as detected by L10 MAb. In the case of B cell lymphomas, they were phenotypically divided into two groups, corresponding to either early or late activated B cells.(ABSTRACT TRUNCATED AT 250 WORDS)

Antibodies, Monoclonal↗
PubMed1986

Characterization of two distinct antigens expressed on either resting or activated human B cells as defined by monoclonal antibodies.

Two antigen systems (L29 & L30) expressed on two distinct human B cell subpopulations were identified by using BL1-4D6 and TB3-7D5 monoclonal antibodies, respectively. L29 was expressed on approximately one-third of B cells in human lymphoid tissues. These B cells associated with L29 were large activated B cells located in the germinal centres of lymphoid follicles. L30, on the other hand, existed on approximately two-thirds of B cells mainly located in the mantle zone of lymphoid follicles, most of which also expressed IgM and IgD on their cell membrane. In addition, L30 was shared on mature granulocytes. With the use of polyclonal activators such as pokeweek mitogen (PWM) and protein A-bearing staphylococci (SAC), L29 antigen was inducible on PWM- or SAC-stimulated B cells in correspondence with the emergence of Tac and T10 antigens of these B cells. In contrast, L30 antigen on the B cells stimulated by the polyclonal activators was decreased in its expression and was finally lost from these B cells. Although none of L29 and L30 was expressed on normal, non-activated human thymus and peripheral T cells, L29 but not L30 was expressed on concanavalin A-activated T cells. Immunochemical studies showed that L30 consist of a single polypeptide with mol. wt of 40,000. L29 antigen is presently under study.

Antibodies, Monoclonal↗
PubMed1986

[A case of rectal cancer with malacoplakia].

A 59-year-old man was hospitalized for rectal bleeding. Barium enema and proctoscopy revealed an elevated tumor, completely stenosing the rectum. The microscopic features of the specimens obtained by proctoscopic biopsy of the tumor comprised aggregations of histiocytes with intracytoplasmic inclusions (von Hansemann cells and Michaelis-Gutmann bodies). Rectal malacoplakia was diagnosed. In the course of laparotomy, the tumor was found to be strongly infiltrated to the pelvis. Thus, radical surgery was not performed, and an artificial anus was made. Excisional biopsy from the tumor tissue revealed the features of well-differentiated adenocarcinoma and malacoplakia. Forty-three cases of malacoplakia of the gastrointestinal tract from a review of the literature including ours were collected and discussed.

Adenocarcinoma↗
PubMed1986

[Respiratory care for pulmonary resection in geriatric patients with bronchogenic carcinoma].

In patients aged over 70 years with bronchogenic carcinoma, respiratory failure following pulmonary resection is the most common cause of death. Before August 1982, 23 patients over 70 were treated surgically at the National Defense Medical College Hospital. Of these 23 patients, 4 died from postoperative respiratory failure, 2 of them dying within 30 postoperative days. Since August 1982, 35 pulmonary resections have been performed in patients over 70 who have been managed with pulmonary rehabilitation, resulting in no deaths from respiratory failure. Pulmonary rehabilitation has also provided a shorter duration of atelectasis and a reduced need for bronchoscopic aspiration. We therefore consider that pulmonary rehabilitation is the respiratory care of choice in elderly patients undergoing pulmonary resection.

Aged↗
PubMed1986

[Clinical evaluation of astromicin by intravenous drip infusion. Report I. Bacterial infections in the field of internal medicine].

Intravenous drip infusion of a new aminoglycoside agent, astromicin (ASTM), was used against various infections in the field of internal medicine, and its clinical efficacy and safety were studied. Clinical effects were evaluated in 105 among 111 patients administered with ASTM. Almost all the patients were given 200 mg of ASTM twice a day by intravenous drip infusion for 60 minutes. Among 105 cases, clinical effects of ASTM were excellent in 15, good in 56, fair in 10 and poor in 24. The number of cases who were judged as excellent or good was 71, and the efficacy rate was 67.6%. Efficacy rates classified by diseases were as follows; 80% (4/5 cases) in sepsis, 55.6% (5/9 cases) in urinary tract infections, and 68.1 (62/91 cases) in respiratory tract infections (RTI). In 91 cases with RTI, clinical effects of ASTM were excellent in 14, good in 48, fair in 9 and poor in 20. The efficacy rates classified by diseases of RTI were 77.3% (34/44 cases) in pulmonary parenchyma infection and 59.6% (28/47 cases) in chronic RTI and others. As subjective and objective side effects, tinnitus and malaise were observed in 5 (4.5%) of 110 patients evaluated for side effects. But, either symptom was mild and disappeared after the end or withdrawal of administration. Slight elevations of S-GPT, BUN and others were observed in 7 cases (6.4%) as abnormal laboratory test values. Safety and efficacy of intravenous drip infusion of ASTM were confirmed.

Adult↗
PubMed1986

Establishment of murine endothelial cell lines that develop angiosarcomas in vivo: brief demonstration of a proposed animal model for Kaposi's sarcoma.

We have reported previously that 100% of the incidence of malignant endotheliomas and angiosarcomas was developed in specific-pathogen-free BALB/c mice given s.c. injections of 1,2-dimethylhydrazine dihydrochloride. These tumors appeared selectively in the liver. In the present study, we have successfully established two cell lines in vitro (D10 and D14) from some of these tumors and have characterized the biological features of these lines. D10 and D14 have a 24- to 26-h doubling time and show a spindle formation in confluent monolayer cultures. The D10 line has a low capability of anchorage-independent growth in soft agar as well as less tumorigenicity in syngeneic mice. When as many as 10(7) D10 cells were injected s.c. into syngeneic mice, only one-half of the mice developed tumors composed of neoplastic endothelial cells with ample vascular lumina of various sizes. The histological appearance of this tumor resembled that of Kaposi's sarcoma as a human counterpart. The D14 line, in contrast, has a relatively high capability to grow in the soft agar culture. However, the tumor growth in vivo was less tumorigenic. None of the mice given injections of less than 10(6) D14 cells developed tumors. Although all of mice given injections of 10(7) D14 cells developed tumors, these tumors regressed completely until the 6th week after inoculation, suggesting that these cells were immunogenic in the transplantation assays using syngeneic mice. These lines may provide useful information for the study of cytological features of vascular tumors.

Animals↗
PubMed1986

[Phase II study of (2''R)-4'-O-tetrahydropyranyldoxorubicin (THP) in patients with advanced gastrointestinal cancer--a report of the Tohoku THP Study Group].

A phase II study of THP was performed in patients with advanced gastrointestinal cancer. The dose schedule was 25 to 40 mg/m2 i.v./cycle repeated every 3 to 4 weeks. One partial (PR) and one minor response (MR) were achieved in 16 evaluable patients with stomach cancer. A case of PR had previously been shown to be resistant to doxorubicin and a case of MR resistant to aclarubicin, respectively. No objective responses were observed in 19 evaluable patients with other tumor sites in the gastrointestinal tract. Forty-eight patients were evaluable for toxic effects. Leukopenia (less than 4 X 10(3)/mm3) occurred in 54% of the patients and was dose-limiting. Thrombocytopenia (less than 10 X 10(4)/mm3) was less frequently observed (13%) than leukopenia. However, no cumulative marrow suppression was observed in repeated courses of the therapy. Non-hematologic toxic effects consisted of gastrointestinal disturbances (23%), hair loss (10%), general malaise (8%), fever (6%), ECG changes (4%) and hepatic dysfunction (2%). Further trials with a high dose schedule (40 mg/m2, q 3-4 weeks) in good-risk patients are necessary to validate the antitumor activity of THP against advanced gastrointestinal cancer.

Adult↗
PubMed1985

Non-Hodgkin's lymphoma of Waldeyer's ring and nasal cavity. Clinical and immunologic aspects.

Twenty-nine cases of non-Hodgkin's lymphoma of Waldeyer's ring (W-NHL) and nasal cavity or paranasal sinus (N-NHL) were studied for tumor-surface marker phenotype and histopathologic correlation with clinical features. Immunostaining procedures on tissue sections by using xenoantisera and monoclonal antibodies to human B- and T-cells enabled the authors to demonstrate precise surface marker phenotypes of tumor cells and, moreover, the histologic localization of normal or neoplastic B- and T-cells in preserving the original structure of lymphoid organs or tumor tissues. In 22 cases of W-NHL, 19 (86%) had B-cell markers and 3 (14%) had T-cell markers, whereas 6 of 7 cases (86%) of N-NHL had T-cell markers. Tumor cells in T-cell lymphomas in W-NHL and N-NHL reacted with antibodies to peripheral T-cells except one case of W-NHL. Rappaport "histiocytic" subtype was heterogeneous with respect to both surface marker characteristics and morphologic features, i.e., seven had B-cell markers and four had T-cell markers, and they were all subdivided into "large cell" or "large cell, immunoblastic" in Working Formulation and "large cell" or "pleomorphic" in Lymphoma Study Group classification. The actuarial survival curve for all T-cell lymphoma patients was characterized by a rapid initial decline and a subsequent plateau, which contained two of the long survivors. In contrast, the B-cell lymphoma group had a more graded decline. The median and actuarial survivals of the T-cell lymphoma group were far inferior to those for the lymphoma group that expressed B-cell markers.

Adolescent↗
PubMed1985

Multiple fungal aneurysms: report of a rare case implicating steroid as predisposing factor.

A 61-year-old man with a ruptured aneurysm of the anterior communicating artery developed hemorrhagic cerebral infarction 22 days after an operation associated with occlusion of the right angular artery. Angiograms further revealed sequential changes of intracranial vessels including progressive obstructions of the right anterior and middle cerebral arteries and four aneurysms on the left pericallosal artery. Subsequently, the patient became comatose and died. At autopsy the newly formed aneurysms originating from the left pericallosal artery were of fungal (Phycomycetes) origin and numerous nonseptate, branching hyphae were also seen in the obstructed cerebral arteries. Intracranial fungal aneurysms are reviewed in the literature and the pathogenesis in the present case is discussed.

Adrenal Cortex Hormones↗
PubMed1985

Primary oligodendroglioma of the lateral ventricle with computed tomographic and positron emission tomographic evaluations.

Two cases of primary intraventricular oligodendrogliomas are presented. Total excisions of well-demarcated large tumors in the lateral ventricle were successfully performed in young women by means of a frontal transventricular approach. An evaluation by computed tomography and positron emission tomography was attempted to obtain definite diagnosis of not only the location of the tumor but also the histologic grade of malignancy.

Adolescent↗
PubMed1985

Construction of rat-mouse T-cell hybridomas that constitutively produce rat IL-2.

We have established rat-mouse T-cell hybridomas that constitutively produce rat Interleukin-2 (IL-2). T-cell hybridomas cannot be boosted to a higher level of IL-2 production by Con A stimulation. IL-2 prepared from T-cell hybridomas and from Con A activated rat spleen cells was partially purified using Ultrogel AcA 54 chromatography and ion exchange chromatography on Mono Q or chromatofocusing on Mono P. When analyzed on Mono P, IL-2 activity derived from IA2-B10 T-cell hybridoma eluted as a single peak with pH range 6.9-7.1, whereas IL-2 derived from Con A activated spleen cells resolved into four peaks within the following pH range: 7.1-7.2, 6.5-6.6, 6.1-6.2, and 5.6-5.7. Neuraminidase-treated IL-2 derived from Con A activated spleen cells resolved into single peaks appearing in the pH range 7.1-7.2. In contrast, neuraminidase treatment did not change the elution profile of IL-2 derived from the IA2-B10 hybridoma. IL-2 activity derived from the 3D6-B1 T-cell hybridoma also eluted as a single peak with the pH range 7.1-7.2. Neuraminidase treatment did not change the elution profile of IL-2. These data demonstrate that heterogeneity of IL-2 might be due to differences in the degree of glycosylation of IL-2 and differences in the sources of T-cells from which the IL-2 has derived.

Animals↗
PubMed1985

A novel cell-surface antigen expressed on most leukocytes and a minor cortisone-resistant population of thymocytes in rats: characterization by monoclonal antibodies.

A cell-surface antigen on rat lympho-hemopoietic cells was determined by using a monoclonal antibody, R2-1B3 (1B3). The 1B3 antibody, when tested for its reactivity with different hemopoietic cells by cytofluorography with a FACS analyzer, labeled more than 80% of lymph node, spleen, and bone marrow cells and 10-20% of thymus cells. Cytofluorographic analysis performed on purified rat T cells, B cells, macrophages, and granulocytes demonstrated that the antigen defined by 1B3 was readily detectable on all of these cell types, with the greatest expression on B cells. A minor population of thymocytes that were labeled by 1B3 appeared to be cortisone-resistant and were located mainly in the thymic medulla. These 1B3 positive thymic cells seemed to be functionally more mature than 1B3-negative thymus cells as suggested by the fact that the cytotoxic treatment of thymus cells with 1B3 antibody and complement (C) resulted in significant reduction of their responsiveness to phytomitogens and lymphokines derived from concanavalin A (con A) activated rat spleen cell cultures. Immunochemical data showed that 1B3 antibody recognized the broad ill-defined band with a molecular weight of 32K to 47K daltons as estimated by SDS-polyacrylamide gel electrophoresis. These data indicate that the 1B3 defined antigen is distinct from other, previously reported, antigens on rat lymphoid cells including leukocyte-common (L-C) and MRC OX-22 antigens, and that this 1B3 antibody is a useful reagent for analyzing the intrathymic differentiation of T cells in rats.

Animals↗