Sera and synovial fluids of patients with rheumatoid arthritis were studied for the presence of heterophile antibodies to sheep and bovine erythrocytes by means of hemolysis in agar gel. It was demonstrated that 18 of 146 sera had hemolytic antibody titers of 160 or more; all 18 (12%) against sheep and 8 (6%) against bovine erythrocytes. Of 31 synovial fluids examined, 5 showed hemolysin titers of 40 or more; all 5 (16%) against sheep and 3 (10%) against bovine erythrocytes. These heterophile antibodies were shown to belong to IgM and/or IgG class. Absorption and inhibition studies revealed that antibodies of 10 positive sera and 2 synovial fluids were of Forssman specificity and antibodies of 6 sera and 3 synovial fluids were of Hanganutziu-Deicher specificity. Two remaining sera were shown to contain a mixture of Forssman antibodies and immune anti-B antibodies.

Absorption↗

PubMedDate not supplied

Studies on immune complexes in rheumatoid arthritis.

Sera and synovial fluids of patients with rheumatoid arthritis were studied for immune complexes by means of the anti-antibody inhibition test; approximately 40% of these specimens were shown to contain immune complexes. To study the nature of antigens participating in the formation of complexes under investigation, the anti-antibody inhibition test was repeated after addition of various soluble antigens to the complex-positive rheumatoid arthritis sera. This resulted in conversion of some sera from inhibitory to noninhibitory. It was found that of 15 positive rheumatoid arthritis sera, 7 had complexes formed by heterophile, Hanganutziu-Deicher antigen and of 12 positive sera tested, 2 had complexes formed by human IgG.

Antigen-Antibody Complex↗

PubMedDate not supplied

Rapid injection of indocyanine green dye for evaluation of choroidal venous circulation.

PURPOSE: The purpose of this study was to investigate the effects of rapid intravenous administration of a small bolus of indocyanine green (ICG) dye on early phase findings of ICG angiography. METHODS: Rabbit eyes were used. Ten normal eyes, 5 eyes with occlusion of vortex veins, and 5 eyes with chorioretinal atrophy were examined by ICG angiography, and early-phase findings after a rapid injection of a small bolus of dye were compared with those obtained with an ordinary injection. RESULTS: In normal eyes, after the rapid injection it was possible to visualize some small bright fluorescent boluses in the choroidal veins from 6 to 12 seconds after injection, while with the ordinary injection they were unclear. In eyes with occlusion of vortex veins, the rapid injection resulted in visualization of the choroidal veins with delayed filling against the background, and the region of the choroidal veins with delayed filling could easily be determined. In eyes with chorioretinal atrophy, only the rapid injection clearly revealed the choroidal veins with delayed filling. CONCLUSIONS: The rapid injection made it possible to detect the choroidal veins with delayed filling that were not clearly and easily visible using an ordinary injection. Rapid injection of ICG dye is useful for a detailed examination of choroidal circulation.

Animals↗

PubMedDate not supplied

Factors related to home discharge of cerebrovascular disease patients: 1-year follow-up interview survey of caregivers of hospitalized patients in 53 acute care hospitals in Japan.

In Japan, many disabled elderly people remain hospitalized for long periods. Cerebrovascular diseases (CVD) are the most causes responsible for such disability. To examine the predictors of home discharge in CVD patients, we performed a 1-year follow-up interview of the main caregivers of patients hospitalized with a CVD event. The initial cohort consisted of CVD patients hospitalized in all the second level emergency and general hospitals in Ibaraki Prefecture in February 1992 (N=888 patients in 53 hospitals). In the following year, we performed an interview survey of the main caregivers of these CVD patients. The survey items included the characteristics of the patients (including medical and socioeconomic conditions), caregivers, and family members. The final study population included the main caregivers of 187 home patients and 90 institutionalized patients. We compared these two groups in terms of predictors of discharge to home. The results of multiple logistic regression analysis showed that the following seven factors were related to home discharge; better baseline activities of daily living (ADL), larger improvement in ADL, larger family size, spouse as the caregiver, caregiver without a full-time job, better economic status of the caregiver, and sources of the patient's income. Our study indicated that the caregiver's conditions were closely related to home discharge of the CVD patients. More attention should, therefore, be centered on the caregiver's well-being and economic aspects as well as the patient's conditions in order to encourage home discharge of stroke patients.

Journal Article↗

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The ultrastructure of lymphatic vessels in the human middle ear with cholesteatoma.

Lymphatic vessels were few in the edematous portion of the cholesteatoma perimatrix of the human middle ear. But the lymphatic vessels contained mucus and consisted of degenerated endothelial cells. Edema of the perimatrix was considered to be caused by the degeneration of lymphatic vessels. Normal lymphatic vessels were found in the fibrous perimatrix, while macrophages infiltrated and phagocytosed cell debris. In addition, regenerating blood vessels were observed. Degeneration of connective tissue due to lymphatic edema and its repair were both present in the perimatrix. The degeneration of perimatrix was hypothesized to be caused by confined mucous epithelium behind the cholesteatoma matrix.

Cholesteatoma↗

PubMedDate not supplied

Immunological analysis in xenogeneic fetal porcine liver transplantation into a beagle.

BACKGROUND/AIMS: While allogeneic organ transplantation has been performed safely, a major barrier in xenogeneic transplantation is how to inhibit hyperacute rejection. METHODOLOGY: We challenged xenogeneic fetal liver transplantation from pig to dog. The graft was investigated by immunohistochemical analysis on recipient's IgG, IgM and C3. RESULTS: In 1 of 4 cases, the graft escaped hyperacute rejection for about 4 hours after transplantation, however, the recipient died next day due to hemorrhage from the torn capsule of the liver due to the arterial blood pressure of the recipient. Histologically, the parenchyma showed good countenance and no congestion nor hemorrhage was shown in the vessels. On immunohistochemical analysis, canine IgG, IgM and C3 were deposited on the sinusoidal epithelium of the fetal liver more moderately than that of adult control. Fetal porcine liver showed less expression of major histocompatability complex class I antigen than that of the adult one. CONCLUSIONS: We consider that the hyperacute rejection occurred more slowly in xenogeneic fetal liver transplantation than in the adult one due to not only less expression of major histocompatability complex class I, but also lower expression of the epitope recognized by a natural antibody of the recipient.

Animals↗

PubMedDate not supplied

Tissue typing by means of mixed agglutination.

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Agglutination Tests↗