Search PubMed⌕ Search

Biomedical subjects

K Kano

Publications and source records attributed to K Kano.

At least 199 records · Page 11Linked to original sources
PubMed1988

Phenotypic and functional analyses on T-cell subsets in lymph nodes of MRL/Mp-lpr/lpr mice.

By means of killing and/or FACS sorting the double-negative (DN) Lyt2-, L3T4- cells, Lyt2+ or L3T4+ cells and B220- cell populations were separated from T-cell-enriched lymph node (LN) cells of 4- to 5-month-old MRL/Mp-lpr/lpr mice. These highly purified cell populations were examined for their proliferative responses, interleukin 2 (IL2) production and expression of IL2 receptor (IL2R) in response to phorbol myristate acetate (PMA) and the calcium ionophore A23187 (A2) or PMA plus concanavalin A. The DNT-cell population was unable to respond to the stimuli and did not express IL2R. Thus the DN T cells, the major population responsible for the lymphadenopathy, possess fundamental defects in signal transduction as well as in the IL2-IL2R-mediated function. On the other hand, Lyt2+ or L3T4+ T cells obtained by sorting or B220- cells purified by the sorting after killing B220+ cells, exhibited proliferative responses indistinguishable from that of LN cells of the congenic MRL/Mp-+/+(+/+) mice. These cells also expressed IL2R after stimulation, however, the amount of IL2 produced was significantly lower than that produced by congenic +/+ cells. This suggested that phenotypically normal Lyt2+ or L3T4+ T cells of lpr LNs also possess a partial defect in the signal transduction system for IL2 production under the influence of the lpr gene.

Animals↗
PubMed1988

[Survival rate].

Explore the source record for details and available documents.

Data Interpretation, Statistical↗
PubMed1987

Distribution of serotonin-immunoreactive paraneurons in the lower urinary tract of dogs.

Morphological and quantitative studies were made on serotonin-containing paraneurons throughout the lower urinary tract in male and female dogs. Using an anti-serotonin antiserum, the cells were consistently demonstrated to be dispersed in the epithelium from the vesico-urethral junction to the external urethral ostium. They occurred most frequently in the urethra proximal to the urogenital diaphragm in both sexes. The total number of the serotonin-immunoreactive cells in the urethra was estimated to be 36.2 X 10(4) (SD 9.9 X 10(4] in the male (n = 3) and 15.6 X 10(4) (SD 2.1 X 10(4] in the female (n = 3). Besides the urethra, the prostate and vaginal vestibule contained several serotonin-immunoreactive cells. The urethral serotonin cells were basically bipolar basal-granulated cells that extended the basal cytoplasm to the basement membrane and reached the lumen with an apical process. Modified cell shapes were, however, also frequent, and included bifurcated apical and/or basal processes or a laterally directed basal process. Occasional serotonin cells possessed a threadlike basal process with varicosities and a terminal bouton, reminiscent of a neuronal process. Immunoreactivity for chromogranin A, a carrier protein common to endocrine paraneurons, was demonstrated in all of the urethral serotonin cells. The chromogranin A-immunoreactive granules accumulated more densely in the basal and perinuclear regions of the cell. It is hypothesized that the serotonin-immunopositive paraneurons may receive chemical and/or physical information from urine and, in response to it, secrete serotonin which presumably causes the contraction of the musculature of the urethra.

Animals↗
PubMed1987

Studies on endometrial cells in human pregnancy by monoclonal antibodies.

By immunizing mice with endometrial cells from pregnant women, we obtained two hybridoma clones, 9D3 (IgG1) and 9E3 (IgG3), secreting monoclonal antibodies (MoAbs) that selectively stained endometrial stromal cells having prolactin in their cytoplasm, but did not stain glandular epithelium nor small lymphoid cells. From examination of tissues, cells and cell lines, it was found that 9E3 antigen was broadly distributed among various mesenchymal cells, while 9D3 antigen was distributed on a more restricted spectrum of mesenchymal cells, skin basal cells and a promonocytic cell line (U937). Neither of these antibodies stained peripheral blood cells, bone marrow cells or endometrium of the proliferating phase. Furthermore, 9D3 MoAb reacted with all melanoma cell lines and squamous cell lines examined. The antigenic molecule that was recognized by 9D3 MoAb had a molecular weight of 94,000.

Animals↗
PubMed1987

Expression of heterophile, Paul-Bunnell and Hanganutziu-Deicher antigens on human melanoma cell lines.

Expression of heterophile antigens was studied on 6 human melanoma cell lines. Paul-Bunnell and Hanganutziu-Deicher (H-D) antigens, but not Forssman antigen, were demonstrated on these cell lines. H-D antigen was also demonstrated on melanoma cells isolated from primary and metastatic lesions. Evidence was also presented that HLA class I but not class II (DR and DQ) molecules are expressed on these cell lines. H-D antibodies of IgG class were detected in 10 of 23 (42%) melanoma patients suggesting the possibility that H-D antigen might have been expressed in an immunogenic form in the patients.

Antibodies, Heterophile↗
PubMed1987

Interleukin-2 responses of MRL/lpr mouse splenocytes and lymph node cells induced by TPA and A23187.

Treatment of splenocytes and lymph node cells of 5 month-old MRL/lpr mice with TPA induced IL-2-dependent proliferation of the cells in the presence of CA++. The induced response was inhibited completely by a monoclonal antibody to IL-2 receptor. The combination of TPA and A23187 in the lpr cells induced both proliferation and production of IL-2 in a Ca++-dependent fashion. The proliferative response of the lpr cells was equivalent to that of congenic (MRL/+/+) or normal cells, but the quantity of IL-2 secreted from the lpr cells was significantly less than that of the controls. Actinomycin D, but not mitomycin C, blocked IL-2 secretion from the treated lpr cells indicating de novo synthesis of IL-2 mRNA in the cells. Thus the lpr lymphocytes can be activated to proliferate in response to IL-2, yet they do not secrete IL-2 at a normal level even if activation signals are transmitted into the cells.

Animals↗
PubMed1987

Mechanism of penile erection in the dog. Pressure-flow study combined with morphological observation of vascular casts.

A pressure-flow study of the corpus cavernosum penis in the dog was performed during the flaccid, tumescent, erect and rigid stages. Perfusion was selectively made into the penile deep artery. The erect stage was induced with the local administration of papaverine hydrochloride, and the rigid stage by electrostimulation of the ischiocavernous muscle during the erect stage. Concurrently, vascular casts of the penis at each stage of erection were observed under a scanning electron microscope for the anatomical analysis of the mechanisms controlling cavernous pressure. In the pressure-flow study, it was demonstrated that a combination of decreased arterial resistance and restricted venous outflow was essential to both cause and maintain the erect state. The rigid stage was generated by contraction of the ischiocavernous muscle tightly compressing the crus penis. In the rigid stage, the cavernous pressure was elevated as high as five times the perfusion pressure; nevertheless, no backflow from the cavernous sinus to the deep artery was recognized. By use of the casts, it was confirmed that venous outflow restriction in the erect and rigid stages was due to compression of the postcavernous venules and penetrating veins in the tunica albuginea. The deep artery was occluded in the tunica albuginea in the casts made during the rigid stage, suggesting that the backflow from the corpus cavernosum penis to the deep artery was prevented by this occlusion. Therefore, in the rigid stage, the arterial and venous occlusion isolated the cavernous sinus from the systemic circulation; this mechanism seems to account for the maintenance of the extremely high cavernous pressure.

Algorithms↗
PubMed1987

Expression and function of class II antigens on gastric carcinoma cells and gastric epithelia: differential expression of DR, DQ, and DP antigens.

The expression of human histocompatibility leukocyte antigen class II antigens on gastric epithelia and gastric carcinoma (GaCa) was studied with the use of murine monoclonal antibodies to DR, DQ, and DP antigens. DR and DP antigens but not DQ antigens were demonstrated in fundic glands of normal gastric epithelia, and DP+ cells were located in part of the DR+ epithelia. Of 15 GaCas examined, 11 expressed DR antigen, and the degree of the expression varied considerably among the specimens. DP antigen was found in 3 of the 11 DR+ carcinomas, and the DQ antigen was found in one of the 3 DR+ DP+ specimens. Thus the expression of class II antigens in normal gastric epithelia and GaCas appears to be in the order of DR, DP, and DQ. Studies on 3 GaCa cell lines (Kato III, MKN28, and MKN45) demonstrated that 1 line (Kato III) expressed DR antigen only, and the remaining lines were negative. Interferon (IFN)-gamma treatment enhanced the expression of DR antigen on Kato III cells and induced expression of DQ and DP antigens. The IFN-gamma treatment also induced expression of DR antigen but not DQ or DP antigens in 1 of the 2 negative cell lines. The induction of the class II antigens by IFN-gamma was shown to be dose dependent. However, maximal induction of DQ and DP antigens on the Kato III cells and DR antigens on MKN45 cells required 10 times more IFN-gamma than that needed for the maximal expression of DR antigen on Kato III. Northern blot analyses of cytoplasmic RNA from these cells were in agreement with and affirmed the above-described expression of the class II antigens on the cell lines. The DR antigen on the Kato III cells was capable of stimulating allogeneic lymphocytes in MLR, and its stimulatory activity was significantly enhanced by IFN-gamma. These results demonstrated a differential expression of class II antigens in the "DR, DP, and DQ order" in normal gastric epithelia, GaCa cells, and GaCa cell lines, suggesting different mechanisms acting discordantly on the expression of each of these antigens and that the DR antigen on the GaCa cell lines possesses MLR-stimulatory ability.

Animals↗
PubMed1987

Immunological studies on Kawasaki disease: II. Isolation and characterization of an immunosuppressive factor in acute phase sera.

Acute phase serum from a patient with Kawasaki disease possessed strong inhibitory activity for the proliferative response of Con A-stimulated peripheral blood mononuclear cells. The inhibitor was fractionated step-wise by means of DEAE-Sephadex A-50 ion exchange chromatography followed by Sephadex G-100 gel filtration and high performance liquid chromatography. A major protein of 140 kD with 2000 times greater inhibitory activity than the original serum was identified in the final fraction. Immunization of mice with this fraction resulted in the production of three hybridoma clones secreting monoclonal antibodies (MoAb) of IgG1 class which blocked the inhibitory activity of the fraction. Two of these MoAb recognized the same epitope of the inhibitory factor, while the remaining MoAb was directed to a different epitope. Western blot analysis of acute phase sera by the MoAb demonstrated the presence of 140 kD molecules in 43 of 55 patients.

Acute-Phase Reaction↗
PubMed1987

[Erectile mechanism studied using penile vascular casts in the dog].

The possible mechanism of penile erection was discussed based on the findings obtained by the scanning electron microscope observations of the penile vascular casts in the dog. Polsters protruding into the lumen of the distal helicine arteries regulate blood flow into the cavernous spaces. The drainage veins from the corpus cavernosum penis arose on the dorsal surface and crept on the corpus until changing direction perpendicularly. This suggested that these veins were efficiently compressed between the tunica albuginea and the corpus cavernosum penis during erection. For a high pressure to be maintained in the cavernous spaces during erection, a closed system separated from the systemic circulation must be required.

Animals↗