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Biomedical subjects

K Kai

Publications and source records attributed to K Kai.

At least 91 records · Page 5Linked to original sources
PubMed1988

Effects of leukocidin from Fusobacterium necrophorum on bovine peripheral leukocytes in vitro.

Partially purified leukocidin from Fusobacterium necrophorum damaged bovine peripheral leukocytes as demonstrated by trypan blue staining. Granulocytes were most and T-lymphocytes least sensitive to the leukocidin. Heating for 30 min at 60 degrees C completely inactivated the leukocidin. The cytotoxicity of the leukocidin could be neutralized by homologous anti-leukocidin. Scanning electron microscopy of the exposed cells revealed an apparent destruction of the cell membranes, loss of the microvilli and smoothing of the cell surfaces.

Animals↗
PubMed1988

The effects of malotilate on hepatic drug metabolizing systems in different strains of rats.

1. In Sprague-Dawley (SD) rats treated for 7 days with malotilate (MAL:250 mg/kg, p.o.), cytochrome P-450 and b5 contents, aminopyrine N-demethylase and heme oxygenase activities were significantly increased. In Wistar rats, cytochrome b5 content and heme oxygenase and delta-aminolevulinic acid synthetase activities were found to be significantly increased. 2. Among the antipyrine metabolites excreted in urine during the 24 hr after antipyrine (100 mg/kg, i.p.) administration, norantipyrine increased significantly in Sprague-Dawley rats, while a significant increase of 4-hydroxyantipyrine was observed in Wistar rats. 3. The serum dimethadione/trimethadione ratio was only found to be significantly increased in Sprague-Dawley rats. 4. These results indicate that malotilate may have inducible effects on hepatic drug metabolizing enzymes, and that it affects the various cytochrome P-450 isozymes from different strains of rat in different ways.

Alkaline Phosphatase↗
PubMed1988

Avulsion of the hamstring muscles from the ischial tuberosity. A report of two cases.

In two rare cases with avulsion of the hamstring muscles from the ischial tuberosity without fracture of the ischium, the clinical features were: (1) sudden onset of pain in the buttock; (2) difficulty on standing after trauma; (3) a palpable defect and tenderness on the area distal from the ischial tuberosity; and (4) weakness of flexion of the knee with a loss of normal outline of the hamstring muscles on the dorsal aspect of the knee. There was no roentgenologic evidence of fracture of the ischium. Surgical repair with reattachment of avulsed muscles to the ischium and proximal tendinous sheaths of the muscles restored function or corrected deformity.

Adolescent↗
PubMed1986

Relationship between the cellular resistance to Friend murine leukemia virus infection and the expression of murine leukemia virus-gp70-related glycoprotein on cell surface of BALB/c-Fv-4wr mice.

Fv-4r is a dominant resistance gene which controls resistance of mice to exogenous infection with ecotropic murine leukemia viruses. Cell lines with various degrees of resistance to ecotropic Friend murine leukemia virus infection were established from BALB/c-Fv-4wr mice which are partially congenic with BALB/c. The degree of resistance of these cell lines correlated well to the amount of glycoprotein with mol wt 80 K on cell surface. The resistance of cells was reduced by the treatment of glycosylation inhibitors, tunicamycin and 2-deoxy-D-glucose. The results indicate that the Fv-4 resistance is ascribed to the unique glycoprotein on cell surface.

Animals↗
PubMed1984

Isolation of paralysis-inducing murine leukemia viruses from Friend virus passaged in rats.

Four clones of murine leukemia viruses (PVC-111, PVC-211, PVC-321, and PVC-441) were isolated from a paralyzed Fischer rat which had been infected with rat-passaged Friend leukemia virus. PVC-211 and PVC-321 viruses induced hind leg paralysis in rats and killed them within 1 month, and PVC-441 did so within 2 months after infection, whereas PVC-111 did not within 4 months. PVC-321 and PVC-441 but not PVC-111 virus grew well in brain and spinal cord media. The viral antigens were found often in glia cells and rarely in neurons of the rats infected with each of these PVC viruses. All of the PVC viruses induced neuronal degeneration but neither inflammation nor leukemic infiltration in the spinal cord. The isolated viruses were all ecotropic and NB-tropic. Age dependency of the susceptibility of rats to paralysis induction was observed.

Animals↗
PubMed1983

Strain difference in immune response of the rat to NRK cells infected with spleen focus-forming virus of the Friend leukaemia virus complex.

Five rat strains were studied for immune responsiveness to SFFV-NRK, a normal rat kidney cell line non-productively infected with spleen focus-forming virus (SFFV) of the Friend leukaemia virus (FLV) complex. Antisera from ACI, JAR, Fischer and SD strains precipitated SFFV-specific gene product, gp55, whereas those from LEW did not. In the cross of Fischer X LEW, immune responsiveness to gp55 was controlled by a single or a few dominant genes. The immune responsiveness was neither related to the susceptibility to FLV infection, nor to the amount of SFFV-related RNA in spleen cells. Unresponsiveness of LEW rats to gp55 was not absolute; when LEW rats were immunized with FLV preparations from infected mice or xenotropic virus-infected NRK cells, they produced antibody that could precipitate gp55.

Animals↗
PubMed1976

Mouse strain resistant to N-, B-, and NB-tropic murine leukemia viruses.

Mouse strain G was studied for its susceptibility to various strains of murine leukemia and sarcoma viruses. Both N- and NB-tropic Friend leukemia viruses neither induced splenomegaly nor grew efficiently in strain G mice. Using the XC test, cultured embryo cells were found to be resistant, but not absolutely, to all the tested viruses, N-tropic AKR virus, N- and NB-tropic Friend leukemia viruses, NB-tropic Rauscher leukemia virus, B-tropic WN1802B virus, NB-tropic Moloney leukemia and sarcoma viruses, and N-tropic Kirsten sarcoma virus, although the resistance to Moloney leukemia and sarcoma viruses is sometimes not as strong as that for other viruses. Thus, the strain G mice are unique among mouse strains because they show resistance that is not related to the N-B tropism of murine leukemia viruses.

AKR murine leukemia virus↗