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Biomedical subjects

K Kaden

Publications and source records attributed to K Kaden.

18 recordsLinked to original sources
PubMed1998

Epstein-Barr virus infection after kidney transplantation.

Besides the well-known association of Epstein-Barr virus (EBV) and lymphomas developing posttransplantation, there are only a few data concerning the prevalence of EBV antibodies (abs) in organ donors and recipients, the posttransplant development of antibody titer in the recipient population, the extent of EBV-IgM seroconversion, and the clinical and laboratory signs associated with infection. Therefore, in a retrospective analysis, we evaluated the data of 119 organ donors and 215 kidney graft recipients who received their kidney allografts between 1993 and 1995. A total of 665 sera were quantitatively tested by means of immunofluorescence assay for EBV abs against capsid (CA; IgM, IgG), early (IgG), and nuclear (IgG) antigens. Recipient sera were obtained for testing before kidney transplantation (KTx) and at post-KTx days 32, 67, and 649 (mean values). Pre-KTx all organ donors and 98.65% of the recipients wre EBV-CA-IgG positive, only in two cases was there a D+/R- (donor/recipient) combination leading to primary EBV infection. In comparison with the organ donors, in kidney graft recipients the pre-KTx IgG antibody titers (geometric mean) against EBV-CA were significantly elevated (1:428 vs 1:574; U-test). Post-KTx, EBV-CA-IgM seroconversion was observed in 29.5% (52 out of 176) of first graft recipients and in 47.8% (11 out of 23) of regrafted patients up to the last follow-up day 649. In 219 out of these 52 recipients, IgM abs again cytomegalovirus (CMV) could also be detected; the other 23 did not develop CMV-IgM abs. The most frequently associated clinical signs were fever (74%), elevated aminotransferases (57%), and deterioration of graft function (26%). In the EBV-IgM+/CMV-IgM+ group there were significantly more rejection therapies than in the EBV-IgM+/CMV-IgM- group. With respect to the severity of EBV infections, 68% were asymptomatic or mild, 27% moderate, and 4% (only 1) severe. Thus, besides the post-KTx CMV monitoring, EBV monitoring should also belong to the follow-up checks.

Adult↗
PubMed1987

Thyroid lid surgery.

A retrospective study of 58 thyroid patients undergoing eyelid surgery for thyroid related lid malposition is reported. A treatment strategy is suggested, based on the results of this experience. The following points are stressed: (1) The importance of recognising and relieving the inferior rectus tethering component of upper lid retraction when present. (2) The usefulness of a scleral graft in lower lid retractor recession. (3) The inadequacy of lateral tarsorrhaphy in relieving lid retraction but its value in camouflage.

Adult↗
PubMed1986

Normal values and membrane markers of theophylline-sensitive and theophylline-resistant human T-lymphocytes.

In 32 healthy adults between 20 and 50 years, the number of theophylline-resistant T-lymphocytes (T-res) was determined to be 51 +/- 4% or 1,161 +/- 326 per microliter, and that of theophylline-sensitive T-lymphocytes (T-sens), 11 +/- 2% or 252 +/- 95.--The decrease in number of rosette-forming cells after incubation with 1 mM theophylline was not caused by reduced vitality (51Cr-release test). The testing of various theophylline concentrations (1, 3, 5, and 10 mM) did not result in a changed count of T-sens. By a variation of the lymphocyte: sheep erythrocyte ratio and of incubation temperature it was found that T-sens possess high-affine and T-res low-affine receptors for sheep erythrocytes. While about 30% of the cells of both subpopulations proved to have Fc-IgM-receptors, among T-sens a significantly higher number of cells carried Fc-IgG-receptors than among T-res (29% vs. 10%).

Adult↗
PubMed1986

[Longitudinal studies in children with pyelonephritis and glomerulonephritis with special reference to selected immune parameters].

In children with a glomerulonephritis the immune laboratory parameters used by us showed deviations from the norm which are necessary to be pursued. This particularly concerns the differentiation of B- and T-cells, the determination of the subpopulations of the T-cells, furthermore also the control of the serum-IgM as well as the investigation of complement and C-factors 3 and 4, respectively. The laboratory methods mentioned deserve to be compared with and related to clinical, paraclinical and renal bioptic data of the GN-patients. Such correlations promise deeper insights into the pathogenic connections of certain forms of glomerulonephritis and possibly also as statement concerning the control of therapy.

Child↗
PubMed1986

[In vitro sensitivity of T lymphocytes to theophylline in healthy adults and patients following cadaver kidney allotransplantation].

In the peripheral blood of healthy adults the number of theophylline-resistant T-lymphocytes (T-res) is 51 +/- 4% and 1,161 +/- 326/microliter, respectively, and the number of theophyllins-sensitive 11 +/- 2% and 252 +/- 95/microliters. The membrane markers show a heterogeneous distribution. The quotient from T-res/T-sens is 4.9 +/- 1.3. Within the T-sens 14% are CD4+ and 30% CD8+, 29% carry Fc-IgC and 34% Fc-IgM-receptors. Thus the T-res in their netto-function were helper cells and the T-sens suppressor/cytotox cells. Before the transplantation the preoperative ratio of the two subpopulation is significantly diminished (Q = 3.66 +/- 1.53), however, prognostic statements cannot be deduced from this. Activations of the immune system (rejection crises, cytomegalovirus infections) are accompanied by significant diminutions of the T-sens, whereby the T-res/T-sens-Quotient increases. Thus they are unequivocally included into immunoregulatory processes.

Adolescent↗
PubMed1984

[Effect of plasmapheresis treatment on immunoglobulin and immune complex levels in patients following kidney transplantation].

Five patients who received cadaver kidneys between May 1982 and January 1983 in the Kidney Transplant Centre in Berlin were subjected to two plasmapheresis (= pph.) treatments in addition to basic immunosuppression with Prednisolone and Azathioprine. The decision to use pph. was due to the presence of donor-specific, complement-dependent lymphocytotoxic antibodies (51Cr release test) in the recipient's serum taken immediately before transplantation. The 1st pph. was carried out on the 1st or 2nd day after operation and the 2nd pph. between the 2nd and 4th day. The quantity of plasma exchanged was between 1.6 and 3.1 1 per pph. Four of the five transplants commenced functioning after 12 to 47 days, and one transplant had to be removed. Frequent measurement of the immunoglobulin and immune-complex levels in the serum revealed drastic reduction due to pph. The concentration of immunoglobulin (G, A, M) was reduced by 42-55% after the 1st pph. and by 20-35% after the 2nd. Whereas the IgM level was normalized after a few days, the levels of IgG and IgA only rose again 2-4 weeks later. The immunodeficiency induced by means of pph. and immunosuppression is accompanied by an increased risk of infection. It is therefore considered important that an adequate anti-infectious treatment including i. v. human gammaglobulin be administered parallel to pph. The final evaluation of the efficacy of pph. in protecting transplants will depend on further studies.

Adolescent↗