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Biomedical subjects

K James

Publications and source records attributed to K James.

At least 271 records · Page 15Linked to original sources
PubMed1975

Normal and pathological serum levels of alpha2-macroglobulins in men and mice.

The serum levels of alpha2-macroglobulin have been measured in normal men and mice and in a number of immunopathological conditions. Normal human concentrations are high in youth, reach their minimum in middle age, and gradually increase with old age. In all age groups the mean is higher in the female than in the male. Conversely, in normal mice the alpha2M level is low in youth, maximum in middle age, and shows a slight depression with old age, and the levels are frequently higher in males than in females; there are also strain variations. In human immunopathological conditions, there are some deviations from the normal alpha2M level but these are seen to be changes from the normal distribution of values around the mean, rather than significant elevation or depression of mean values. In some disease states studied there are differences between the sexes in the deviation from normal. "Abnormal" strains of mice had alpha2M levels within the range exhibited by "normal" mice but changes in the levels are seen in mice with various myelomas.

Adolescent↗
PubMed1975

Effect of Corynebacterium parvum on the class and subclass of antibody produced in the response of different strains of mice to sheep erythrocytes.

Several strains of mice were injected with sheep erythrocytes (SRBC) using C. parvum as adjuvant. The adjuvant effects on the amounts of class and subclass of antibody produced were ranked in the order IgG2b greater than IgG2a and IgM greater than IgG1. In addition, these effects were shown to vary depending on the time of administration of C. parvum relative to antigen. C parvum was shown to have no adjuvant effect on the response of congenitally athymic mice when given at the same time as the antigen, SRBC. On the basis of the reported observations it is suggested that certain of the adjuvant effects of C. parvum require T-cell function.

Adjuvants, Immunologic↗
PubMed1975

The effect of human alpha 2-macroglobulin on the restoration of humoral responsiveness in x-irradiated mice.

The effect of human alpha 2-macroglobulin on the recovery of the humoral immune response in X-irradiated mice has been assessed by measurement of antibody-forming cells in the spleen 5 days after challenge with sheep erythrocytes. The capacity of this protein to promote the recovery of immune responsiveness was variable, and appeared to be influenced by the strain and sex of the mice, and the dose of alpha2M given.

Animals↗
PubMed1975

The association of alpha2-macroglobulin with lymphocyte membranes in chronic lymphocytic leukaemia and other disorders.

The association of alpha2-macroglobulin (alpha2M) with the surface membranes of human peripheral blood lymphocyte preparations has been investigated by the direct immunofluorescent technique. The percentage (and total number) of lymphocytes with detectable alpha2M on their surface is significantly increased in chronic lymphocytic leukaemia. The incidence of alpha2M-positive cells in normal and pathological conditions closely parallels that observed with conjugated antiserum to the kamma and gamma light chains of human immunoglobulin. It bears no relationship however to the plasma alpha2M levels or to the age of the donor. Additional blocking studies with aggregated human IgG, soluble antigen-antibody complexes and the F(ab')2 moiety of the anti-alpha2M, and indirect immunofluorescent studies with the latter, indicate that the fluorescence observed is not due to interaction of the conjugated reagent with Fc or C3 receptors.

Adult↗
PubMed1975

The effect of anti-alpha2-macroglobulin on K-cell cytolysis and T- and B-cell rosette formation.

The effect of antisera to human alpha2-macroglobulin (alpha2M) on the ability of human peripheral blood lymphoid cells to lyse antibody coated target cells (exert a K-cell effect) and form T(E) and B(EAC) rosettes has been investigated. Pretreatment of lymphoid cells with these antisera inhibited their K-cell activity but had no effect on their capacity to form T or B rosettes. The inhibitory activity of the anti-alpha2M sera was always reduced following absorption on a CnBr column to which human alpha2M had been coupled. The absorbed antibody, recovered by acid elution reacted strongly with human alpha2M in gel diffusion precipitin analysis and caused a highly significant inhibition of K-cell cytotoxicity. It was further shown that the inhibitory activity of the anti-alpha2M was localized exclusively in the IgG fraction and was greatly reduced following pepsin digestion.

Antibodies↗
PubMed1974

The effect of transplanted methylcholanthrene induced fibrosarcomata and Corynebacterium parvum on the immune response of CBA and A-HeJ mice to thymus dependent and independent antigens.

The effect of transplanted syngeneic methylcholanthrene induced fibrosarcomata on the primary immune response of CBA and A/HeJ mice to standard doses of alum BSA, SRBC and SIII has been investigated. In animals with established fibrosarcomata the responses were (with one exception) either normal or elevated. Cell transfer studies in sublethally irradiated syngeneic recipients confirmed that the spleens from tumour bearing mice were capable of responding effectively to all 3 antigens. In animals simultaneously challenged with viable sarcoma cells and antigen the response to alum BSA was suppressed while those to SRBC and SIII were often enhanced. Furthermore the secondary response of A/HeJ mice to BSA was also suppressed by the simultaneous injection of viable fibrosarcoma cells. The administration of C. parvum 3 days after antigen had a variable effect. Nevertheless in a number of cases it significantly increased the primary response to all 3 antigens. It also inhibited the growth of the CBA fibrosarcoma but was without effect on the A/HeJ fibrosarcoma.

Animals↗