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Biomedical subjects

K Jackson

Publications and source records attributed to K Jackson.

At least 55 records · Page 3Linked to original sources
PubMed1998

Immunoblotting of multiple antigenic peptides.

Multiple antigenic peptides (MAPs) can be efficiently separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to a nitrocellulose membrane for subsequent use in immunoblot (IgG and IgM). MAPs can be stained by Coomassie and silver on SDS-PAGE as well as by Fast Green on an immunoblot. Affinity immunoblotting for analysis of antibody clonotype distribution has also been carried out using MAPs. High performance liquid chromatography purification of the MAPs is mainly responsible for their migration as sharp bands in SDS-PAGE and immunoblotting.

Amino Acid Sequence↗
PubMed1998

Growth hormone, sexual maturity and steroids in male carp (Cyprinus carpio).

Samples of pituitary, blood plasma and gonad were taken from male carp. The growth hormones (GH) in the pituitary and plasma were measured in fish of various body weights (BW) and degrees of gonad development, and compared with the levels of 17 beta-estradiol (E2), testosterone (T), 17 alpha-hydroprogesterone (17-P), 11-ketotesterone (11-KT) and progesterone (P) in the testes and plasma. The gonadosomatic index increased rapidly with BW from 100 to 600 g, and then decreased at 900 g. The pituitary GH did not change with BW, but plasma GH was higher in fish weighing 300 +/- 50 and 600 +/- 50 g, than in fish weighing 900 +/- 50 g. In fish weighing 150 +/- 50 to 300 +/- 50 g, the level of T rose significantly in the testes (2.27 ng g-1) and plasma (1.3 ng g-1); E2 was very low in both testes (0-30 pg g-1) and plasma (11-28 pg ml-1), increasing as BW rose from 150 to 600 g. The level of P rose mainly at BW of 300 +/- 50 and 600 +/- 50 g: from 0 to 25 ng g-1 in the testes and from 0 to 17 ng ml-1 in the plasma. The level 17-P rose from 2.5 to 20 ng g-1 in the testes at 600 +/- 50 g BW, but no significant change was recorded in the plasma. The level of 11-KT rose significantly in the tests of fish at 300, 600 and 900 g (0.5-6 ng g-1). The application of different steroids (E2, T and 17-P) on a primary culture of pituitary cells led to the release of GH. Release was significantly higher (P < 0.05) after 4 h at steroid concentrations of 10(-6) M.

17-alpha-Hydroxyprogesterone↗
PubMed1998

Utilization of Child Health Services during the first 18 months of life: aspects of health surveillance in Swedish preschool children based on information in health records.

The aim of this study was to evaluate some aspects of care given within the preventive Child Health Services (CHS) during the first 18 months of life. A national random sample performed on child health records of 172 Swedish preschool children born between 1982 and 1987 was analysed regarding services recorded as having been provided and used within and beyond the national programme of health surveillance. Most families had made visits within the core programme of health surveillance to an optimal or at least sufficient extent. First-time parents visited the CHS more frequently than did more experienced parents. Procedures within the programme, such as growth monitoring, hip examination and immunizations, were documented to have been optimally performed on a majority of the children. Conversely, screening for hearing impairment and assessment of developmental milestones were performed less frequently, as were health information and postnatal parental education. To improve the quality of care, national recommendations ought to be more specific regarding both the performance and the documentation of the service.

Child Health Services↗
PubMed1998

Multiple cranial nerve palsies complicating retrobulbar eye block.

This report details a case of accidental brainstem anaesthesia complicating retrobulbar block for cataract surgery in an 83-year-old man. Prompt diagnosis and treatment resulted in a successful outcome. The many possible presentations of brainstem anaesthesia are described and causative mechanisms are discussed.

Aged↗
PubMed1997

Understanding and enhancing compliance with the second dose of hepatitis B vaccine: a cohort analysis and a randomized controlled trial.

OBJECTIVE: To determine the predictors and extent of noncompliance with a second dose of hepatitis B vaccine and the effectiveness of a compliance enhancement strategy. DESIGN: Cohort analysis and randomized clinical trial. SUBJECTS: A total of 256 consecutive adults attending a sexually transmitted diseases clinic from October 1992 to July 1993 who were seronegative for hepatitis B virus and agreed to receive hepatitis B vaccination. SETTING: Hamilton, Ont. INTERVENTION: Subjects were followed up for 4 months. Those who did not return for the second dose of vaccine by 6 weeks after the first (2 weeks overdue) were randomly assigned to the enhanced intervention group (telephone and mail reminders) or the regular intervention group (mail reminder only). Subjects were considered noncompliant if they did not return for the second dose by 4 months after the first. RESULTS: The risk of not returning for the second dose of vaccine within 4 months after the first was strongly and linearly associated with level of education (p = 0.004). The noncompliance rate among those with less than a grade 10 education was 50%, grade 10-13 education 34%, some college education 15% and some university education 9%. In the randomized controlled trial the enhanced intervention group had twice the compliance rate of the regular intervention group (48% v. 25%; p = 0.008). Subjects with no postsecondary education were highly responsive to the enhanced intervention (relative risk 2.1; p = 0.02) compared with those with a higher level of education (relative risk 1.0; p = 1.0). CONCLUSION: Hepatitis B vaccine recipients with lower educational levels are at increased risk of noncompliance with the second dose of vaccine but are highly responsive to telephone reminders.

Adult↗
PubMed1997

Cochlear implants in children: what constitutes a complication?

The surgeon must ultimately accept the responsibility for any complications that occur as the result of a cochlear implant. Listening to the cochlear implant team members and responding to their needs may enhance the child's progress. Surgical complications, (i.e., skinflap problems, infection, and facial paralysis) are indeed infrequent, but nonsurgical problems are not. Surgical and nonsurgical experiences were reviewed in 55 children. Ages ranged from 23 months to 18 years at the time of cochlear implantation, which occurred from 1984 to 1995. There were no surgical complications. However, the most common surgical obstacle was ossification, which was present in 40% and undetected by computed tomographic scanning in 16.3% of children. Ossification occurred at the round window and scala tympani in 32.7% and involved the cochlea more extensively in 7.3% of children. In only one child was the cochlea entirely ossified. There were, however, many nonsurgical problems that were viewed as complications in patient management. The single most important complication was device failure. This occurred in 10.9% (5/46) of children with the Cochlear Corporation multichannel implant. Head banging and other temper tantrums, parental interference with rehabilitation, socioeconomic factors, poor compliance by the family unit, equipment problems, educational deficiencies, and impatience with habilitative training were some of the other problems.

Adolescent↗
PubMed1997

Regulation of decidual cell and chorion cell production of interleukin-10 by purified bacterial products.

PROBLEM: To determine whether cultured human decidual cells and chorion cells produce interleukin-10 (IL-10) after incubation with purified bacterial products. METHOD OF STUDY: Decidual cell cultures and chorion cell cultures were established by standard techniques. With confluence, monolayers of each culture were incubated with purified bacterial products, including various concentrations of lipopolysaccharide (LPS), lipid A, and lipoteichoic acid (LTA) for 16 hr in quadruplicate. Culture supernatants were collected and assayed for immunodetectable IL-10 by enzyme-linked immunoadsorbent assay (ELISA). RESULTS: Both decidual cell cultures and chorion cell cultures produced significant quantities of IL-10 after stimulation with LPS, lipid A, and LTA. Cultures of decidual cells produced more IL-10 than did chorion cell cultures. CONCLUSIONS: Our data indicate that both maternal decidual cells and fetally derived chorion cells can produce IL-10 after incubation with bacterial virulence factors. This finding contrasts with our previous findings in which chorion cells did not produce IL-10 after stimulation with IL-1 beta, suggesting that chorion cell production after incubation with bacterial products is independent of IL-1 beta. We speculate that the contribution of anti-inflammatory IL-10 production by human gestational tissues to the inflammatory process in these tissues may be overcome or abrogated by the pro-inflammatory process.

Bacterial Infections↗
PubMed1997

Using nursing standards to evaluate competency.

A competency evaluation program uses nursing standards to link performance management, quality improvement and education to patient care outcomes. This program enables nurse managers to capitalize on the data already available to them and helps meet the Joint Commission on Accreditation of Healthcare Organizations standards. An orientation competency assessment and an annual competency evaluation are provided.

Accreditation↗
PubMed1996

Use of data from a hospital online medical records system by physicians during preanesthetic evaluation.

OBJECTIVE: There is no data on the use of hospital-wide online medical record (OLMR) systems by anesthesiologists. We measured how often anesthesiologists accessed the OLMR database maintained by the hospital, how often data was copied from this database into the clinic's computer system, and how much data was copied. METHODS: In a preanesthetic evaluation clinic that has a computerized evaluation system designed for physician-entered data, a graphical user-interface prototype link provided access to the hospital OLMR database for users and was studied over a 37-day period. The software allowed the user to search the OLMR system by patient name, retrieve a text listing of the patient's record, and then copy and paste desired information into the forms of the preanesthetic system. Using embedded routines, we recorded how many times physicians searched for and retrieved medical records from the hospital OLMR database, as well as how many times they copied data to the preoperative database. As a measure of how much data was copied, the number of characters was also recorded. RESULTS: Of 1,080 patients evaluated in the clinic during the study period, electronic searches of the hospital OLMR database for 221 patients (20.5%) were noted. Of these searches, 208 (94.1%, or 19.3% of 1,080 patients) successfully retrieved data from the patient's record. Data was copied for 170 patients - 81.7% of the successful searches. Of 7,525,153 characters retrieved, 262,269 were copied-an average of 1,543 characters per instance of copying. CONCLUSION: We conclude that anesthesiologists, given even crude graphical access to a hospital OLMR data-base, will retrieve and copy data, potentially increasing the accuracy of the medical records and saving time.

Anesthesiology↗
PubMed1996

Growth hormone, gonad development, and steroid levels in female carp.

The pituitary and plasma growth hormone (GH) levels of female carp (Cyprinus carpio L.) were measured in fish of various sizes and degrees of maturity, and were matched against the levels of 17 beta-estradiol (E2), testosterone (T), 17 alpha-hydroxyprogesterone (17-P), and progesterone (P) in the ovary and plasma. The short-term action of the above hormones and 17 alpha, 20 beta-dihydroxy-4-pregnen-3-one (17,20-P) on the release of GH was examined in vitro in primary culture pituitary cells. The gonadosomatic index (%GSI) increased rapidly in specimens when they had attained 900 +/- 50 g body weight (BW). The pituitary and plasma GH levels increased between 150 and 600 g BW (when oocytes reached the stage at which lipoprotein appeared in the cytoplasm), but at 900 g BW (with oocytes in vitellogenesis) the plasma GH dropped, while pituitary GH remained high. E2 increased with BW, reaching its maximum at 600 and 900 g BW in the ovary and plasma, respectively. Similar patterns were found in the levels of T and P, both hormones reaching their maximum levels at 900 g BW. The level of 17-P was very low and did not increase in proportion to BW. The application of various concentrations of different steroids on a primary culture of pituitary cells led to release of GH. The highest degrees of release were obtained from 10(-6) and 10(-7) M E2, 10(-6) M T, 10(-7) M 17-P and 10(-8) M 17,20-P. In all these cases, hormone treatment effected higher release of GH than was found in the control. A model of the relationship between GH and the steroids associated with maturation is proposed.

Animals↗
PubMed1996

Token reinforcement, choice, and self-control in pigeons.

Pigeons were exposed to self-control procedures that involved illumination of light-emitting diodes (LEDs) as a form of token reinforcement. In a discrete-trials arrangement, subjects chose between one and three LEDs; each LED was exchangeable for 2-s access to food during distinct posttrial exchange periods. In Experiment 1, subjects generally preferred the immediate presentation of a single LED over the delayed presentation of three LEDs, but differences in the delay to the exchange period between the two options prevented a clear assessment of the relative influence of LED delay and exchange-period delay as determinants of choice. In Experiment 2, in which delays to the exchange period from either alternative were equal in most conditions, all subjects preferred the delayed three LEDs more often than in Experiment-1. In Experiment 3, subjects preferred the option that resulted in a greater amount of food more often if the choices also produced LEDs than if they did not. In Experiment 4, preference for the delayed three LEDs was obtained when delays to the exchange period were equal, but reversed in favor of an immediate single LED when the latter choice also resulted in quicker access to exchange periods. The overall pattern of results suggests that (a) delay to the exchange period is a more critical determinant of choice than is delay to token presentation; (b) tokens may function as conditioned reinforcers, although their discriminative properties may be responsible for the self-control that occurs under token reinforcer arrangements; and (c) previously reported differences in the self-control choices of humans and pigeons may have resulted at least in part from the procedural conventions of using token reinforcers with human subjects and food reinforcers with pigeon subjects.

Animals↗