A continuous cell line has been established from larval fat body tissues of the cerambycid beetle Xylotrechus pyrrhoderus Bates. These cells were cultured in MGM-450 medium. The cell line, designated as XP-1, showed a heterogeneous population consisting of spherical and spindle-shaped cells with some capacity to adhere and a doubling time of 5 d. The chromosome number of the cell line ranged from 18 to 42 with a mode of 20. Isozyme analysis showed that the cells had patterns distinctive from those of other insect cell lines. The cells were sensitive to insect hormones, and when continuously treated with 20-hydroxyecdysone and juvenile hormone, they assumed a floating elongated-spindle shape and became strongly adherent, respectively.

Animals↗

PubMedDate not supplied

Computed tomography in the assessment of pancreatic tumor response after intraoperative radiation therapy.

Computed tomography was used in 7 patients given intraoperative electron beam therapy for advanced carcinoma of the pancreas. The local tumor response was studied quantitatively by defining the tumor contour in consecutive CT scans and then estimating the tumor volume. The maximum diameters of the tumor in 3 planes (X, Y and Z) were also estimated. There was evidence of initial tumor regression in all patients during the first few months after the treatment. No specific behaviour of the diameters in the X, Y and Z planes could be detected. Later on, regrowth of the tumor could sometimes be observed, preferably in one of the 3 planes. On the whole, CT was found to be a useful tool for assessing tumor response to this form of therapy.

Aged↗

PubMedDate not supplied

Analysis of interaction between molecules of Bombyx mori nucleopolyhedrovirus IE-2 using a yeast two-hybrid system.

Baculovirus IE-2 protein is one of well-known transactivators. In this report, we demonstrate that Bombyx mori nucleopolyhedrovirus (BmNPV) IE-2 interacts with itself. Several clones were obtained from a yeast two-hybrid screening system using IE-2 as bait and were found to encode IE-2 protein. Nucleotide sequencing of these clones showed that they contained C-terminal regions in common. Further analyses suggest that BmNPV IE-2 protein interacts with itself through 80 amino acid residues of coiled-coil domain in C-terminus.

Animals↗

PubMedDate not supplied

A femoral vein catheter with immobilized urokinase (UKFC) as an antithrombotic blood access.

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Acute Kidney Injury↗

PubMedDate not supplied

[Dementia and amyotrophy in Kufs disease. The adult type of neuronal ceroid lipofuscinosis].

A 26-year-old housewife, born of consanguineous parentage, began to have gait and speech disturbance. Her brother had died from suffocation because of dysphagia. At thirty-two, she developed difficulty in swallowing, clumsiness and incontinence. When she was thirty-six she had pseudobulbar palsy, vertical gaze paresis, hyperreflexia and muscular atrophy of the upper half of the body. CT scan showed cerebral atrophy. Her mental function progressively deteriorated and amyotrophic lateral sclerosis associated with dementia was suspected. She died at the age of thirty-seven. Diagnosis was made only by autopsy. There was no particular general pathologic finding excepting aspiration pneumonia. Microscopical examination revealed numerous distended neurons with accumulation of light brown pigments by Luxol fast blue/H & E stains, especially in hypothalamus, substantia nigra and nuclei of oculomotor nerves. To a lesser extent such neurons were noted ubiquitously. The stored material was mainly composed of lipofuscin and ceroid. Ultrastructurally they presented the various structures which have previously been reported, except for finger print profiles. The pigmentary deposits were shown to be immunoreactive with polyclonal antibody directed against amyloid beta-protein.

Adult↗

PubMedDate not supplied

Malignant meningioma with a new intracytoplasmic inclusion body.

A 55-year-old man presented with a frontal mass lesion, which histologically consisted of pleomorphic tumor cells with necrotic foci and abundant mitotic figures, showing a meningothelial pattern focally. In addition, many eosinophilic round- or irregular-shaped bodies were seen not only in tumor cell cytoplasm but also in between the tumor cells. By electron microscopy, the tumor cells showed a minimal adherence to each other and the intercellular spaces were wide. Tumor cells had numerous branching cytoplasmic processes and some of them were united by desmosomal junctions. These features suggested that the tumor was a malignant variety of arachnoid trabecular cell meningioma. The inclusion bodies consisted of homogeneous fine granular material and were not surrounded by microvilli. This type of inclusion body has not been reported in meningiomas in the past. The significance and possible pathogenesis of the inclusions are discussed.

Cell Division↗

PubMedDate not supplied

A chondromyxoid fibroma-like tumor of the cranial convexity: immunohistochemical and ultrastructural study.

A rare chondromyxoid fibroma-like tumor arising from the temporal bone in a 49-year-old man is described. This case appears to be only the second reported of a cranial lesion of the tumor for which diagnosis could be confirmed by immunohistochemistry and electron microscopy.

Chondroblastoma↗