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Biomedical subjects

K Ishihara

Publications and source records attributed to K Ishihara.

At least 757 records · Page 42Linked to original sources
PubMed1982

Protection by nicotinamide against streptozotocin-induced reduction in pancreatic TRH.

The concentration of thyrotrophin-releasing hormone (TRH) immunoreactivity was determined in pancreatic islets and acini in the rat. In addition, time-course changes in TRH in response to an iv injection of streptozotocin (65 mg/kg body weight) with or without nicotinamide (500 mg/kg body weight) were examined in the whole pancreas. Furthermore, pancreatic TRH was measured in diabetic rats treated with insulin for 3 weeks. The TRH concentration in rat islets was 42-fold higher than in exocrine glands, indicating that the majority of pancreatic TRH is of islet origin. The mean concentration of pancreatic TRH decreased to 60 and 65% of the respective control values at 4 and 7 h after administration of streptozotocin, respectively. AT 24 h, it fell to 10% of control values without significant changes in TRH levels in the hypothalamus and gastrointestinal tract. In contrast, no significant change in pancreatic TRH was noted in rats given combined treatment with streptozotocin and nicotinamide. The injection of streptozotocin alone resulted in severe hypoglycaemia at 7 h and hyperglycaemia at 24 h, whereas neither resulted from the combined treatment. Insulin therapy had no influence on the decreased TRH concentrations in the diabetic pancreas. These results suggest that TRH may be localized to the B cells of pancreatic islets, and that the marked reduction in TRH in diabetic pancreases is not a metabolic consequence of insulin deficiency.

Animals↗
PubMed1982

[Sleepiness scale and an experimental approach (author's transl)].

The Kwansei Gakuin Sleepiness Scale (KSS) is a self-rating scale which is developed after the Stanford Sleepiness Scale (SSS) and using Thurstone's method of equal-appearing intervals. The present study investigated whether the KSS correlates with vigilance task performance, fatigue test and oral temperature, and whether circadian variation of the KSS ratings is demonstrated during 40 hours sleep deprivation. Six student subjects employed in this study were given performance test, self-ratings and temperature measurement at every 3 hour for 3 days. On Day-2, sleep deprivation was carried out to all subjects. The results showed that mean KSS ratings highly correlated with correct detection rates on the vigilance task, fatigue test and oral temperature, and that the change of KSS ratings demonstrated not only the effect of sleep deprivation but also the circadian variation in sleepiness.

Adult↗
PubMed1982

[Malignant melanoma in Japan: unique distribution and effect of DAV chemoimmunotherapy].

This study, based on a co-operative group project involving 4 major medical institutes in Japan presents the first survey of malignant melanoma (MM) patients (157 cases) where an attempt was made to systemically evaluate the distribution of primary MM, and the response to DAV-chemoimmunotherapy. The distribution of primary MM in Japan is unique in a sense that the MM involving the lower extremities occupied more than 50% of the total male and female cases. The commonest type and site of involvement is the acrolentiginous MM involving the plantar area (30%). The regimen of our group included the combination of DTIC, VCR and ACNU, a new nitrosourea with or without immunoadjuvants OK-432, PSK, or NK-421). By a historical comparison, the DAV-treated group showed a better prognosis in the survival rates of overall (Stages I-IV) and disseminated (Stages III-IV) patients than those of the non-DAV group. However, the effect of combined immunoadjuvants was not statistically significant, though OK-432 showed a significant inhibition of lymphopenia which always occurred during DAV therapy.

Adjuvants, Immunologic↗
PubMed1981

Efficacy of anti-ulcer drugs on the recovery of gastric mucosal glycoproteins with aspirin-induced gastric damage in rat.

The efficacy of two anti-ulcer drugs, Cimetidine and Cetraxate, on the mucus glycoproteins of gastric mucosa in the aspirin-induced gastric damage was studied in rats. Simultaneous or previous oral administration of Cimetidine or Cetraxate with aspirin reduced the diminution of the mucus glycoproteins which was occurred by aspirin administration. The recovery of the content of mucus glycoprotein in drug dosed rats occurred within 3 h after aspirin dosing and was nearly 90% of control at 5 h in all cases. Single administration of Cetraxate or Cimetidine produced an increase in the mucus glycoprotein content greater than that of the untreated control. Although a macroscopical method for the measurement of gastric damage was applied to this work, neither erosions nor linear ulcers were observed in all cases except the single administration of aspirin. The biochemical method used should be able to assess the efficacy of drugs on the mucosal lesion which cannot be expressed as the ulcer index.

Animals↗
PubMed1981

Some properties of a hexadecane hydroxylation system in rabbit intestinal mucosa microsomes.

Among many different tissues of rabbit, hexadecane hydroxylation activity was found in only small intestine and liver microsomes. Both activities for hexadecane and decane hydroxylation in intestinal microsomes were significantly higher than those in liver microsomes. In contrast, the hydroxylation activities of p-xylene, benzo(a)pyrene, and decanol, and the demethylation activity of aminopyrine in the former were much lower than those in the latter. The intestinal microsomes converted [1-14C]hexadecane to cetyl alcohol, but not further to palmitic acid. Hexadecane hydroxylation activity was found to be markedly stimulated by non-ionic detergents such as Triton X-100, Nonident P-40, and Emulgen 913. Phosphatidylcholine and phosphatidylethanolamine stimulated the activity to a lesser extent. The hydroxylation was inhibited by various aliphatic hydrocarbons with carbon numbers larger than 10, but not by aromatic and polycyclic hydrocarbons. Hexadecane hydroxylation activity was solubilized from the intestinal microsomes and reconstituted with a partially purified cytochrome P-450 fraction, and intestinal NADPH-cytochrome c reductase, or spinach ferredoxin and ferredoxin-NADP reductase. The chromatography of the crude cytochrome P-450 preparation on hydroxylapatite separated at least two cytochrome P-450 fractions; one preferentially hydroxylating hexadecane, and the other preferentially hydroxylating myristic acid. The results suggest that rabbit intestinal mucosa microsomes had a cytochrome P-450 species specialized for hexadecane hydroxylation.

Alkanes↗
PubMed1981

Intrasplenic pseudocyst of the pancreas.

We describe a rare case of pancreatic pseudocyst developing in the spleen as a sequel to chronic pancreatitis in the absence of a recognizable pseudocyst in and around the pancreas. We assume that pancreatic secretions escaped through a tight adhesion between the tail of the pancreas and the splenic hilum.

Humans↗
PubMed1981

Electrophysiologic studies of Jervell, Lange-Nielsen syndrome.

Electrophysiologic studies were performed in two sibling with Jervell, Lange-Nielsen syndrome. Electrophysiologic parameters were all within normal limits except for prolongation of effective refractory period of the ventricular myocardium. This finding may suggest one of electrophysiologic mechanisms underlying QT prolongation, that is, delayed ventricular repolarization.

Child↗