Biomedical subjects

K Ishihara

Publications and source records attributed to K Ishihara.

At least 523 records · Page 29Linked to original sources

PubMed1990

Heartworm migration toward right atrium following artificial pulmonary arterial embolism or injection of heartworm body fluid.

Heartworms harboring in the pulmonary arteries migrated toward the right atrium following insertion of dead heartworms or heartworm-like silicone tubes, or intravenous injection of body fluid extract of a female heartworm. The migration occurred within 3 hr (early group) or 1 to 7 days (late group) after insertion of dead worms, 1 to 11 days after insertion of silicone tubes, and immediately after infusion of heartworm-body fluid. The cardiac output decreased to an unmeasurably low level, and the pulmonary arterial pressure was also reduced in the early group. Although the output decreased, the pulmonary arterial pressure and the total pulmonary resistance increased gradually in the late group. In dogs with heartworm migration, in which silicone tubes had been inserted, the changes in cardiopulmonary values were the same as those in dogs of the late group. In dogs to which the body fluid had been administered intravenously, the changes in cardiopulmonary values were well accord with those in the early group. The systemic blood pressure also fell immediately after the administration with the shock-like state. These results suggest that the death of a part of the heartworms may be closely associated with the migration of heartworms toward the right atrium through the pulmonary arterial embolism and/or shock-like reaction by heartworm body fluid.

Animals↗

PubMed1990

Improvement in pulmonary arterial lesions after heartworm removal using flexible alligator forceps.

Improvement of pulmonary arterial lesions after heartworm removal using flexible alligator forceps was investigated by measuring pulmonary arterial pressure (PAP), and by angiographic and histopathological examinations in 11 dogs. PAP obtained immediately after worm removal corresponded well with angiographic abnormalities. In 2 dogs, high PAP immediately after worm removal fell gradually by 12 weeks, and obstructions on angiogram were resolved at 4 to 12 weeks. In 4 dogs, slightly high PAP fell to the normal range at 4 weeks, and angiographic abnormalities were considerably reduced at 4 to 12 weeks. In 5 dogs, PAP returned to normal range immediately after worm removal, and angiographic changes almost disappeared at 4 to 8 weeks. On biopsy immediately after worm removal, samples of the main pulmonary arteries showed severe intimal proliferations with villous or papillary protrusion into the lumen. Autopsy at 12 to 20 weeks indicated that the intimal protrusions were remarkably reduced as compared with the biopsy samples in all cases. However, villous intimal protrusions were seen in the caudal lobar arteries in cases with remaining alive worms. New vessels seemed to develop into thromboemboli with time. From these findings, it is clear that the pulmonary arterial lesions improved after heartworm removal, and the clinical signs disappeared following the improvement in hemodynamics. Aspirin therapy (5 mg/kg/day) for 4 weeks after worm removal in 5 dogs did not improve the intimal lesions as compared to 3 control dogs.

Animals↗

PubMed1990

[Effects of heat treated cells of intestinal lactic acid bacteria in rats fed a deoxycholic acid diet].

Effects of the administration of heat treated cells of intestinal lactic acid bacteria were examined in rats fed a deoxycholic acid (DCA) diet. Male Wistar rats were given a 0.25% DCA diet with or without the heat treated cells of Enterococcus faecalis AD 1001 (EFH-1) or Lactobacillus reuteri AD 0002 (LRH-2) for 4 weeks. Abnormal increases in serum GOT, GPT, UN and lipoproteins were observed in the rats fed the DCA diet. Furthermore, severe lesions in the kidney as well as in the liver were found in these rats. On the contrary, the increases in serum GOT, UN, VLDL and LDL were significantly suppressed, and markedly fewer lesions in the liver and the kidney were observed in the rats fed the DCA diet plus EFH-1 or LRH-2.

Animal Feed↗

PubMed1990

[A rapid diagnosis (SK-013) for periodontitis based on the enzymatic activity of periodontopathic bacteria. Study on the characterization of the SK-013 and the enzyme specificity].

We present studies for development of an enzymatic diagnostic method for periodontitis. A rapid and sensitive diagnostic method named SK-013 was provided by Sunstar Inc. to evaluate the peptidase activities specifically derived from periodontopathic bacteria such as Bacteroides gingivalis, Bacteroides forsythus and Treponema denticola and some strains of Capnocytophaga species. The results obtained indicated the specificity of the enzymatic reaction in this system.

Bacteroides↗

PubMed1990

[A rapid diagnosis (SK-013) for periodontitis based on the enzymatic activity of periodontopathic bacteria. The relationship between the enzymatic activity (SK-013) and B. gingivalis, B. forsythus, T. denticola in subgingival microflora].

SK-013 was developed for rapid detection of the peptidase activity in subgingival plaque samples. The purpose of this study was to determine whether SK-013 could be a marker for the presence of periodontopathic bacteria including Treponema denticola, Bacteroides gingivalis and Bacteroides forsythus which produce trypsin-like enzyme. Subgingival plaque samples were taken with 3 paper points from 10 clinically healthy sites and 30 periodontal diseased sites. The SK-013 activity of plaque sample was assayed and the cells of T. denticola, B. gingivalis and B. forsythus in the sample were counted by immunofluorescence technique. In diseased sites, both the SK-013 activity and the cell counts of these organisms were significantly higher than those in healthy sites. The proportions and cell counts of these organisms and the SK-013 activity were closely correlated with clinical parameters including Gingival Index, Plaque Index, and Probing depth. The correlation between the presence of these organisms and the SK-013 activity was significant. Correlation coefficients between the presence of T. denticola and the SK-013 activity were higher than those of others.

Bacteroides↗

PubMed1990

Antibacterial effects of Listerine on oral bacteria.

To evaluate the efficacy of Listerine, a solution for washing the oral cavity consisting of essential oils (thymol, methanol, eukalyptol) and methyl salicylate, minimum concentrations inhibiting the growth of various microorganisms in the oral cavity and the bactericidal effects on bacteria in the saliva and dental plaque were evaluated in vitro. Listerine inhibited the growth of microorganisms over a very broad range. The minimum concentration inhibiting growth of Listerine was a 4 to 32 fold dilution, 2-4 times as potent as the solution after elimination of active ingredient components, in 38 of 54 bacterial strains, indicating the efficacy of the active ingredient in the inhibition of the growth of microorganisms. Bactericidal action of Listerine against from bacteria isolated from saliva and dental plaque from 5 healthy normal subjects was tested. Listerine exhibited a potent bactericidal effect on bacteria in saliva and dental plaque. Most of the bacteria died after a 30 second exposure to Listerine. According to these results, Listerine appears to be effective as a solution used for cleansing the oral cavity and dentures.

Bacteria↗

PubMed1990

[Durability of bonding between 4-META/MMA-TBB resin to dentin pretreated with 10-3. The effect of 10-3 pretreating period and subsequent glutaraldehyde treatment].

Adhesion durability between dentin pretreated with 10-3 and 4-META/MMA-TBB resin was studied. Reduction of etching periods with 10-3 was not so effective as expected. The weakening of bond strength during immersion in water at 37 degrees C to the dentin pretreated for 1 sec occurred faster than those for either 5 sec or 10 sec. The strength decreased from 12 MPa at 1 day to 9 MPa at 3 months, 3 MPa at 6 months and finally 2 MPa at 1 year in the case of 1 sec pretreated dentin. On the other hand, the strength became half after the storage in water for 1 year in the cases of 5 and 10 sec pretreated dentins. Combination of 10-3 pretreatment and subsequent glutaraldehyde treatment could stabilize the decrease but not completely. SEM and TEM examinations suggested that dentinal collagen exposed by the etching but not entangled and impregnated by poly (4-META-co-MMA) easily deteriorated by water during the longer immersion. Collagen modified with 10-3 and then with glutaraldehyde was also changed by the longer immersion.

Acid Etching, Dental↗

PubMed1990

[Second serogroup of Legionella pneumophila isolated from a patient with fulminant pneumonia].

A 67-year-old female was admitted to our hospital, because of high fever and dry cough. She had undergone semiradical hysterectomy and radiation therapy for carcinoma of the uterine cervix one year previously. Her chest roentgenograms on admission showed lobar consolidation of the left upper lobe. Antibiotics were administered but her general condition and pulmonary consolidation did not improve. As Legionellosis was highly suspected, we performed bronchoscopic examinations. Bronchial mucosa was almost normal with no secretion, and Legionella was isolated from the specimen obtained bronchoscopically. Six days later, L. pneumophila serogroup 2 was isolated and identified from an intratracheal aspiration, and serological diagnosis was made by indirect immunofluorescence antibody. We could also detect the bacteria in the BALF by immunofluorescence microscopy and in the tissue of the TBLB specimen with the ABC method.

Aged↗

PubMed1990

[Lung injuries and serial bronchoalveolar lavage findings in patients with summer-type hypersensitivity pneumonitis].

To evaluate acute lung injuries and their persistence in patients with summer-type hypersensitivity pneumonitis, repeated bronchoalveolar lavage (BAL) and pulmonary function tests were performed. BAL was performed on 36 occasions in 17 patients with hypersensitivity pneumonitis. Nineteen BAL procedures in 16 cases were done during the active phase within the three hospital day and BAL was repeated in 8 cases during inactive phase. Anti-Trichosporon cutaneum antibodies were detected in all of 15 cases examined using the Ouchterlony method and indirect immunofluorescent methods. The number of total BAL cells, lymphocyte and neutrophils were increased in the active phase, and OKT4/OKT8 was quite low (0.39). As the disease became inactive, the number of total BAL cells, lymphocytes and neutrophils decreased. On the other hand OKT4/OKT8 increased quickly. Significantly negative correlations were recognized between the number of BAL lymphocytes and %VC, and lymphocytes and %DLCO4. More improvements in BAL findings, %VC and %DLCO one year after acute episodes were seen in cases that moved house than cases that did not move, and these often relapsed. We concluded that complete clearing of the patient's house or moving out, if necessary, were needed to avoid relapse and persistent lung injuries.

Adolescent↗

PubMed1990

[Platelet-activating factor in Japanese cedar pollinosis].

Japanese cedar pollinosis is a typical allergic disease and has recently become a big social problem. Many population are suffering from this disease every year from the end of February to the beginning of April. In this study, we planned to examine the role of platelet-activating factor (PAF) in this disease, because PAF has been known to be one of the potent chemical mediators in allergic and inflammatory reactions and many evidences indicate that PAF is deeply involved in the pathogenesis of bronchial asthma, a typical allergic disease. We measured the concentrations of the PAF derivative (lyso-PAF) in serum from patients with cedar pollinosis during the pollen season. The level of lyso-PAF in serum from untreated patients with cedar pollinosis (87.8 +/- 8.7 unit.) was significantly higher than that in healthy control (54.9 +/- 7.7 unit.). We also tested the effect of an anti-allergic drug on the level of serum lyso-PAF of cedar pollinosis. Eighteen pairs of serum samples from patients with cedar pollinosis were employed in this study. Lyso-PAF levels after two weeks' therapy with ketotifen (2 mg/day), an anti-allergic drug, decreased the levels significantly (from 82.6 unit to 41.3 unit). These results suggest that PAF could play some important role in cedar pollinosis and that the clinical effect of anti-allergic drug could be partially due to the anti-PAF action.

Adult↗

PubMed1990

[Analysis of anti HMW.MAA antibodies in sera of melanoma patients using antiidiotypic monoclonal antibodies].

The immunogenicity in patients with melanoma of three high molecular weight melanoma associated antigens (HMW.MAA) defined by murine monoclonal antibodies (MoAb) was investigated. For this end sera from patients with melanoma (stage I, IV) and normal individuals were tested by inhibition assay and sandwich assay using antiidiotypic MoAb to anti HMW.MAA MoAb. In inhibition assay, sera were tested for their ability to inhibit the binding of murine antiidiotypic MoAbs to biotin conjugated anti HMW.MAA MoAbs. Some of the sera from patients with melanoma significantly inhibited the binding of antiidiotypic MoAb to anti HMW.MAA MoAb in comparison with that from normal individuals. In sandwich assay, sera were tested for their ability to bind to precoated murine antiidiotypic MoAb and to biotin conjugated anti HMW.MAA MoAbs. Positive findings suggest the existence of anti HMW.MAA antibodies in sera were showed in several sera from the patients, which were less in frequency than that in inhibition assay. These results suggest that HMW.MAA could elicit the immune response in some melanoma patients and also suggest that the assay anti HMW.MAA MoAb in sera will help us to understand the prognosis of the patients and to evaluate the usefulness of treatment with antiidiotypic MoAb as vaccine therapy.

Antibodies, Monoclonal↗

PubMed1990

[Clinical evaluation of 123I-orthoiodohippurate (123I-OIH) for diagnosis of renal function: dose finding study].

The safety, clinical usefulness and the optimal dose of 123I-orthoiodohippurate (123I-OIH) were evaluated in patients with mild renal disorders. 123I-OIH was administered intravenously to 45 patients, and 39 of those patients were analyzed for the clinical usefulness. The numbers of cases analyzed were 10 for 9.25 MBq (0.25 mCi), 18.5 MBq (0.5 mCi) and 74 MBq (2.0 mCi) and 9 for 37 MBq (1.0 mCi). Neither adverse reaction nor abnormal laboratory findings due to 123I-OIH administration were observed in the 45 patients. The clinical usefulness of the drug was evaluated in terms of individual renal function, regional renal functions, renal blood flow, parenchymal function and urinary function, and ability to differentiate between the renal and urinary functions, and the overall clinical usefulness. In the evaluation by the investigators, there were no significant differences in each of these parameters among any of the doses administered. In the evaluation by the Committee, however, the usefulness with 74 MBq superior to those with 9.25 MBq and 18.5 MBq, while there was no significant differences between those with 37.5 MBq and 74 MBq. The qualities of the blood flow phase images, functional phase images, functional images, and the statistical noises of the individual and the regional kidney renograms were also evaluated by the Committee. The image qualities and the statistical noises with 37 MBq were superior to those with 9.25 MBq and 18.5 MBq except for the functional image quality, and there were no significant differences between those with 37 MBq and 74 MBq other than the regional renograms and the functional images, which were superior with 74 MBq.(ABSTRACT TRUNCATED AT 250 WORDS)

Evaluation Studies as Topic↗

PubMed1990

[Three adult intussusception cases with colorectal cancer; CT diagnosis and its characteristic findings].

We have experienced three adult intussusception cases with colorectal cancer. In all three cases CT studies were performed prior to administering a barium enema, where we could make the exact diagnosis of the lesions in both the plain and the contrast studies. In the CT studies, the intussusception lesions showed the characteristic eccentric layered pattern. It was difficult to distinguish the tumors from the edematous intestinal walls at the proceeding portion in two cases. While in one case, the proceeding portion (the tumor itself) was pointed out as the enhanced soft tissue density mass of layered pattern with the irregular margins.

Aged↗

PubMed1990

[A resected case of mediastinal tuberculous lymphadenitis with pericostal tuberculosis].

A case of 22-year-old female with mediastinal tuberculous lymphadenitis and pericostal tuberculosis was reported. Her complaint was right chest pain and subcutaneous mass on the right chest wall. Chest contrast CT showed right paratracheal lymph node swelling with central low density area and surrounding rim enhancement, which has been reported as typical characteristics of mediastinal tuberculous lymphadenitis. Pigeon-egg sized subcutaneous mass with fluctuation was palpable on the right sternal border and the smear of its content showed acid-fast bacilli. In spite of two months therapy with antituberculous drugs, both masses were unchanged in size. The lesions resected surgically, were both encapsulated abscesses containing yellowish pus, and microscopic examination of these specimen disclosed the finding of tuberculosis. Mycobacterium tuberculosis was cultured from contents of both masses. After nine months of anti-tuberculous therapy, no sign of recurrence is observed until now. Both masses were discontinuous and the possibility of lymphangitic spread of organism was speculated as its etiology.

Adult↗

PubMed1990

Ten-year follow-up after valve replacement with the St. Jude Medical prosthesis in children.

Since 1979, 50 children, 4 months to 15 years of age, have successfully undergone cardiac valve replacement with the St. Jude Medical prosthesis (St. Jude Medical, Inc., St. Paul, Minn.). There were 24 boys and 26 girls. The valve replaced was mitral in 28 children, aortic in 15, mitral and aortic in 1, and mitral and tricuspid in 1. A left-sided tricuspid valve was replaced in 3 children. Anticoagulant therapy was maintained in all children; 40 children were treated with warfarin, whereas 10 children who underwent aortic or mitral valve replacement were on a regimen of aspirin combined with dipyridamole. The follow-up period, comprising 224 patient-years, ranged from 1 to 10 years. There were four valve-related complications: one from thromboembolism, two from valve thrombosis, and the other one from prosthetic valve endocarditis. Actuarial rate free from all valve-related complications at 10 years was 84.7%. There were four late deaths: one from valve thrombosis and the others from non-valve-related complications. Actuarial survival rate at 10 years was 90.8%. All surviving children are in functional class I, and no child so far has needed replacement of a prosthesis because of somatic growth. These results indicate that the St. Jude Medical prosthesis is a cardiac valve substitute of choice for valve replacement in children.

Actuarial Analysis↗

PubMed1990

[Effects of acid secretagogues on rat gastric mucus glycoprotein content and gastric mucosal resistance].

In our previous reports, low dose acid secretagogues caused increase in corpus mucus glycoprotein content in rats. This increase was not due to the direct action of protons. The present study was conducted to determine whether this increase has a mucosal function or not Rats were given histamine (0.8 mg/kg) intraperitoneally or tetragastrin (12 micrograms/kg) subcutaneously. One hour following the acid secretagogues administration, the animals were given 1 ml of 30, 40 or 50% ethanol orally and then sacrificed after one hour. Gastric mucus glycoproteins were isolated from the corpus region of stomach. The following results were obtained: 1) Histamine prevented the gastric mucosal damage and the decrease of corpus mucus glycoprotein content induced by 40% ethanol ingestion. 2) Tetragastrin prevented the gastric mucosal damage induced by 30 approximately 50% ethanol ingestion and the decrease of corpus mucus glycoprotein content induced by 40 approximately 50% ethanol ingestion. Based on these results, the increase of corpus mucus glycoprotein content induced by acid secretagogues has a mucosal function. Moreover, tetragastrin strengthened the mucosal defence together with acid secretion.

Animals↗

PubMed1990

[Surgical problems in d-TGA with VSD and PS associated with insertion of tricuspid valve chordae to the infundibular septum--the Rastelli operation by translocation of the infundibular septum].

Twelve (37.5%) out of 32 patients who underwent surgical repair of complete transposition of the great arteries (d-TGA) with ventricular septal defect (VSD) and pulmonary stenosis (PS) from 1978 to 1987 had insertion of tricuspid valve (TV) chordae to the infundibular septum, i.e. superior margin of the VSD, which precluded the ordinary Rastelli type operation. Of these 12 patients early 5 patients (ages: 2-11 mean 7 years) underwent atrial switch operations with VSD closure and pulmonary valvotomy with or without fibromyotomy, and recent 6 patients (age: 4-8, mean 6 years) underwent the Rastelli operation (3 cases) or REV (3) by translocating the infundibular septum with TV chordae to the right ventricular side of the internal conduit. None died in hospital in the former group and 1 in the latter. Pulmonary/systemic ventricular peak pressure ration (Ppv/sv) was 0.75 +/- 0.22 (mean +/- standard deviation) in the atrial switch group v. 0.49 +/- 0.08 in the Rastelli/REV group 1 month postoperatively. The higher Ppv/rv in the atrial switch group was attributed to the pressure gradient across the pulmonary outflow tract, which was 37.8 +/- 16.6 mmHg in the atrial switch v. 16.7 +/- 4.2 mmHg in the Rastelli/REV (p less than 0.05). No pressure gradient was demonstrated between left ventricle (LV) and aorta in the both groups. Pulmonary wedge pressure was higher in the atrial switch group (13.8 +/- 1.9 mmHg) than in the Rastelli/REV (10.0 +/- 2.7 mmHg) (p less than 0.05). Systemic atrioventricular (AV) valve regurgitation was noted in 2 of the atrial switch group but none in the Rastelli/REV.(ABSTRACT TRUNCATED AT 250 WORDS)

Child↗

PubMed1990

[A study on the endometrial movement and serum hormonal level in connection with uterine contraction].

By means of transvaginal ultrasonography, endometrial activity was investigated in its relationship with uterine contraction and ovarian hormones. The following results were obtained. (1) Endometrial movements were classified into two types: Peristaltic movements and undistinguished ones. Peristaltic movements were rhythmic and mainly found in the proliferative stage, directed from the internal uterine os to the fundus. (2) The detection rate and the frequency of peristaltic movements increased towards ovulation, with the highest values at 88% and 3.9/min, respectively. (3) Peristaltic movements rapidly ceased soon after ovulation and there were practically none after the mid-secretory stage. (4) All endometrial movements decreased in the secretory stage and became markedly quiescent after the mid-secretory stage. (5) In the proliferative stage, a positive correlation was found between the frequency of peristaltic movements and the serum estradiol level (p less than 0.001). In the secretory stage, the detection rate was lowered significantly when the serum progesterone level was more than 3.8 ng/ml (p less than 0.01). (6) Peristaltic movements coincided with uterine contractions both in rhythm and duration. As stated above, endometrial movements were strongly affected by hormonal condition, so they could be of value in the prediction and confirmation of ovulation and in the diagnosis of luteal insufficiency and dysfunctional bleeding as well.

Adult↗