Search PubMed⌕ Search

Biomedical subjects

K Irjala

Publications and source records attributed to K Irjala.

At least 181 records · Page 10Linked to original sources
PubMed1980

Marked decrease in serum HDL cholesterol level during acute myocardial infarction.

The concentrations of serum total and HDL cholesterol and triglycerides were determined in 57 patients during the course of AMI. In seven days the concentration of serum cholesterol decreased by 24% and that of HDL cholesterol by 31%. The mean HDL/total cholesterol ratio decreased significantly (p less than 0.01) from 0.163 to 0.145. The magnitude of the change in both HDL and total cholesterol showed a positive correlation with infarction size. The concentration of triglycerides decreased in seven days on the average by 31% but there was great individual variation, which was not dependent on infarction size. Four months after infarction both HDL and total cholesterol as well as triglyceride concentrations had returned to the initial levels. There was a significant negative correlation between the concentrations of HDL cholesterol and triglycerides on admission (r = -0.66) and after four months (r = -0.53) but no correlation after seven days. The results indicate that the determination of serum lipids, including HDL cholesterol, in patients with AMI can, and should be performed on admission to hospital and not at the time of discharge, in order to get reliable estimates of these cardiovascular risk factors.

Cholesterol↗
PubMed1979

Identification of CSF leakage by immunofixation.

The use of immunofixation for identification of the presence of CSF by demonstrating its two electrophoretically separate bands of transferrin has proved to be a valid and rather simple method, particularly when the samples obtained from the nose or ear are very small and contaminated.

Blood Protein Electrophoresis↗
PubMed1979

Immunochemical determination of serum transferrin. Reference values, correlation with serum total iron-binding capacity and value in the diagnosis of iron deficiency anaemia and anaemia of chronic disorders.

Immunologically determined reference values of serum transferrin are presented for adults and children. A good correlation between serum transferrin and total iron-binding capacity values was found. In 2 groups of anaemic patients - 51 patients with iron deficiency anaemia and 45 patients with anaemia of chronic disorders - serum transferrin determination distinguishes the two groups of anaemic patients from normals somewhat better than TIBC determination.

Adolescent↗
PubMedDate not supplied

Time-resolved fluorometry (TRF)-based immunoassay concept for rapid and quantitative determination of biochemical myocardial infarction markers from whole blood, serum and plasma.

We report the development of a time-resolved fluorometry-based immunoassay concept for the rapid measurement of three cardiac markers from whole blood, serum or plasma. Using a universal all-in-one (AIO) dry reagent concept, all the analyte specific reagents are built into a single microtire well, to which an identical assay protocol is applied. Addition of 5-20 microL sample (whole blood, serum or plasma) together with a universal buffer initiates the reaction, which is brought close to equilibrium in 15 min. After the wash step the Eu chelate-derived signal is measured directly from the dried surface. Application of this concept to the three cardiac markers illustrates its ability to provide rapid, highly sensitive and fully quantitative results over a large dynamic range with good reproducibility. Such a performance, especially when using whole blood specimens, is largely a consequence of the inherently fluorescent and stable Eu-chelate employed in the system. Correlation to commercial assays was excellent for all three analytes, as was between-sample matrix correlation using the AIO assays. The presented assay concept enabling a simple automation is particularly suited for point-of-care applications, where the performance characteristics are fully comparable to state-of-the-art central laboratory immunoassays.

Biomarkers↗
PubMedDate not supplied

Design and development of the THYROID system.

This paper discusses the design and development of our decision support system for thyroid function diagnostics, named Thyroid. The objective of our work has been to develop a decision support system for practical use. The Thyroid system has been designed to utilize the existing laboratory test results of the patient. The task of the system is to produce interpretative reports for the clinicians. The system is integrated with the laboratory information system of University Central Hospital of Turku. It has been under clinical testing for more than 2 years with over 1600 actual cases. The system has produced promising results and it has also shown the limitations of the knowledge-based systems in this infrastructure environment.

Clinical Laboratory Information Systems↗
PubMedDate not supplied

Acute prolonged exercise reduces moderately oxidized LDL in healthy men.

We studied the effects of a 2-day walk exercise (6 h+6 h) on the serum concentration of circulating moderately oxidized LDL (LDL baseline conjugated dienes), lipids (total cholesterol, LDL cholesterol, HDL cholesterol, and triglyceride), antioxidants (alpha-tocopherol, gamma-tocopherol, beta-carotene, and ubiquinol-10), and antioxidant potential in serum (S-TRAP) and LDL (LDL-TRAP) in healthy well-trained men. The exercise was performed twice with an interval of 14 days. While 6 h walking the subjects drank 6 cl . kg (-1) water which contained either carbohydrate (CHO trial) or placebo (PLA trial). During the 2-day exercise the level of oxidized LDL decreased by 25 % (p=0.001) in the PLA trial. At the same time serum gamma-tocopherol decreased by 20 % (p=0.049), while the other measured antioxidants remained unchanged and the serum antioxidant potential increased by 22 % (p=0.018). Serum total cholesterol decreased by 3 % (p=0.017), serum triglycerides by 22 % (p=0.001), and LDL-cholesterol by 14 % (p=0.045). HDL cholesterol increased by 9 % (p=0.001). The results in the carbohydrate trial were similar to the ones in the PLA trial. The findings suggest that exercise of long duration but of low, non-exhaustive intensity decreases the concentration of circulating oxidized LDL simultaneously with an increase in serum antioxidant potential in healthy trained men. Carbohydrate ingestion during the exercise does not have any further effect on these changes.

Adult↗
PubMedDate not supplied

Serum iron, zinc, copper, selenium, and bromide concentrations after coronary bypass operation.

The effect of surgical trauma on serum trace elements was studied in 10 patients undergoing coronary bypass surgery. After the initial decrease due to the hemodilution during operation serum iron, zinc, copper and selenium remained depressed for the immediate postoperative period. Zinc was still at significantly lower level 2 months after the operation. Low serum iron values were also observed. Changes in trace element concentrations were parallel with changes in the concentrations of their transport and binding proteins in the serum. The multielemental technique also measured nonessential bromide, which returned to initial levels in 7 days. Effects of trauma on metabolism, acute phase reaction with redistribution of zinc and copper and losses via increased urinary excretion explain the above changes. Development of a subclinical deficiency of zinc and possibly iron is suggested by the persistence of low serum levels during recovery after operation.

Aged↗