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Biomedical subjects

K Inuzuka

Publications and source records attributed to K Inuzuka.

At least 19 recordsLinked to original sources
PubMed1995

Establishment of functional imprinting of the H19 gene in human developing placentae.

We have found that the imprinted H19 gene can be expressed either biallelically or monoallelically in the developing human placentae. H19 biallelic expression is confined to the placenta until 10 weeks of gestation, after which it becomes exclusively maternal, and does not affect allele-specificity or levels of IGF2 expression. The promoter region of H19 is hypomethylated at all stages of placental development, while the 3' portion shows progressive methylation of the paternal allele with gestation. Our observations demonstrate that the establishment of functional H19 imprinting occurs during the early development of the placenta and provide an opportunity to understand the mechanism by which the H19 primary imprint is manifested in somatic cells.

Alleles↗
PubMed1995

A rapid latex immunoassay for the detection of plasmin-alpha 2-plasmin inhibitor complex. Utilization of two monoclonal antibodies differentially recognizing respective components of the complex.

Among six monoclonal antibodies raised against the human plasmin-alpha 2-plasmin inhibitor complex (PPI), three antibodies were found to recognize the plasmin part (group 1) and another three the alpha 2-plasmin inhibitor (alpha 2-PI) part (group 2) of the complex. One of the group-1 monoclonal antibodies, designated JIPPI-3, specifically reacted with a segment of plasmin containing kringles 2 and 3. Although all three group 2 antibodies reacted with both alpha 2-PI and PPI on immunoblotting and ELISA, one of them, JIPPI-50, was unable to react with alpha 2-PI, when the antibody had been covalently conjugated to Sepharose 4B gels and tested for reactivity against the antigens in solution. The results indicated that the epitope for this antibody had been buried in nascent alpha 2-PI, but had been exposed by complex formation with plasmin or by possible conformational changes induced in the alpha 2-PI molecule on insolubilization to nitrocellulose membranes or immunoplates. By utilizing a set of JIPPI-3 and JIPPI-50, individually coated onto latex beads, PPI could be measured in plasma in the range of 0.8-100 micrograms/ml without interference by coexisting plasminogen (120-200 micrograms/ml) or alpha 2-PI (70 micrograms/ml). This measurable range seems to cover the level of PPI clinically observed under hyperfibrinolytic states.

Animals↗
PubMed1995

[The present status and future roles of tuberculosis advisory committees].

We surveyed the present status and future roles of Tuberculosis (TB) Advisory Committees to study the ideal concept of the Committees using questionnaires. After the introduction of the new TB Control Law (which allowed the integration of the Committees), about half of the local governments (prefectures, special cities and special wards in Tokyo) have decreased their committees numbers by a third to two-thirds of the original number of health centers. In order to improve the quality of the Committees, there were additional educational activities suggested by the respondents in the questionnaire. However there were several problems highlighted in the questionnaire, for example a decrease in TB specialists, the aging of Committee members and some Committees with extremely low numbers in reported cases of TB. For the improvement of the Committees both in quality and the range in activities, it is not enough to integrate the Committees. It is recommended that the Committees should be improved in training and education for doctors and staff of the health centers and that they contribute more in comprehensive counter measures against TB in each region. In future the Committees roles should be enlarged and improved in quality, for example, to carry out extra periodic mass screening for TB, and to examine the chest x-rays in mass screenings for lung cancer.

Communicable Disease Control↗
PubMed1991

[Clinical studies and morphological response on ST-95 (fosfomycin)].

Clinical effects of ST-95 (fosfomycin) on infections of the urinary tract were studied on the basis of morphological changes of the urinary bacteria. The subjects were 22 inpatients with complex infections of the urinary tract. ST-95 was dissolved in physiologic saline (2 g/100 ml) and administered intravenously by drip infusion over a 1-hour period twice daily for 5 days. According to the UTI criteria, the treatment was effective in 13 (81%) of the 16 evaluable cases. The present effectiveness in 8 patients with chronic catheterization was 75%. No side effects were observed in any patient. Among the laboratory data, GOT and GPT were slightly increased in 1 patient, but their relations with the treatment were unknown. In one patient (P. vulgaris; MIC: 12.5 micrograms/ml), changes in the urinary ST-95 concentration, viable bacterial count, and morphology of the bacteria were studied after the initial administration. The urinary viable cell count was 1.5 x 10(7) cells/ml before administration, but it was reduced to 1.6 x 10(4) cells/ml at the end of the 1-hour infusion, and decreased progressively thereafter to complete disappearance after 12 hours. Under differential interference microscopy, filamentous debris of bacteria and spherical bacteria with irregular surface were observed from immediately after the end of the administration. By transmission electron microscopy, the cell wall showed a number of projections with formation of vacuolar structures in the space between the cell wall and the irregularly-shaped cytoplasms. In conclusion, the results of administration of ST-95 in complex infections of the urinary tract were satisfactory. The morphological changes of the bacteria suggested that this agent acts at an early stage of cell wall synthesis.

Bacteria↗
PubMed1990

[Morphological study on growth process of primary dentition having distal step of the terminal plane. 1. Growth on the average].

Growth process was analyzed on the primary dentition having bilateral distal step of the terminal plane. Plaster models and cephalometrics of 32 cases were examined. They were taken in the following five developmental periods: Hellman's Dental Ages IIA, eruption of the first molars, eruption of the maxillary central incisors, eruption of the maxillary lateral incisors, and half a year past the eruption of the maxillary lateral incisors. The measurement of plaster models was taken on the mesiodistal crown diameter, the interdental spaces in the primary dentition, the traverse width of the dental arches, and the arch length. Cephalometric analysis was made on 21 linear items together with 6 proportional items, 18 angular items, and coordinates. For a control group, plaster models and cephalometrics of 12 cases having normal occlusion were also analyzed in the same areas as the examined group. These normal cases were chosen according to Kito's criteria of normal occlusion. Comparisons were made between the two groups and the results were as follows: 1. Growth process data obtained from the plaster models 1) There was no significant difference between the mesiodistal crown diameter of the two groups except in the mandibular deciduous central incisors. 2) Concerning the total interdental spaces from the primary canine to the opposite canine, the examined group showed longer spaces in the mandibular dentition (3.03 millimeters on the average) than the control group (1.83 millimeters on the average) (p less than 0.05). 3) Concerning the transverse width of the arches, in the maxillary dentition, the examined group showed a narrower width than the control group through all developmental periods (p less than 0.05), and in the mandibular dentition, the examined group showed wider intercanine width than the control group. However, there was no significant difference between the two groups in the growth process of the transverse width. 4) The examined group showed a longer arch length than the control group in the mandibular dentition in the period of Dental Age IIA (p less than 0.05). 2. Growth process data obtained from cephalometrics 1) Linear growth data in the skeletal pattern (1) The examined group showed a larger dimension in the total cranial base length (N-Ar), the posterior cranial base length (S-Ar), and the posterior facial height (S-Go) than the control group through all developmental periods (p less than 0.05). However, there was no significant difference between the two groups in the quantitative growth.(ABSTRACT TRUNCATED AT 400 WORDS)

Cephalometry↗
PubMed1990

[Morphological study on growth process of primary dentition having distal step of the terminal plane. 2. Individual growth].

In order to analyze individual growth, 10 cases which contained longitudinal data and materials were chosen out of the 32 cases with bilateral distal step that were used in the study of Part I. The control group comprised the same twelve cases that were used in Part I. Observations of plaster models were made on the eruption age and eruption sequence of the first maxillary and mandibular permanent molars and incisors, the mesiodistal crown diameter, the interdental spaces in the primary dentition, and individual growth of the maxillary intercanine width. Cephalometric analysis was made on linear and angular items in which a significant difference between the two groups was found in the study Part I: Growth on the Average. Growth patterns were classified according to profilograms. The relationship between the terminal planes and analyzed items was also discussed. The results were as follows: 1. Individual growth data obtained from plaster models 1) Concerning the eruption age of the permanent teeth, the first maxillary molars of the examined group erupted in 5.84 years on the average. In cases of the control group, 6.42 years was the average. There was a significant difference between the two groups. The maxillary and mandibular central incisors of the examined group tended to erupt slightly earlier than the control group. 2) Concerning the eruption sequence of the permanent teeth, the first maxillary molars or the mandibular central incisors erupted earliest in the examined group, and the first mandibular molars or the mandibular central incisors in the control group. 2. Individual growth data obtained from cephalometrics 1) Concerning skeletal patterns, the examined group showed a significantly larger increase of the lower facial height (NF-Me) than the control group in the period from eruption of the first molars to eruption of the maxillary central incisors. In this period, 8 out of 10 cases of the examined group and 3 out of 12 cases of the control group showed the maximal growth change. 2) Concerning denture patterns, the maxillary incisor height (Ul-NF) increased markedly in the period from eruption of the first molars to eruption of the maxillary central incisors. This was found in 8 cases of the examined group and 4 cases of the control group.(ABSTRACT TRUNCATED AT 400 WORDS)

Cephalometry↗
PubMed1987

[Morphological studies of Escherichia coli in the urine of patients with acute simple cystitis treated with cefbuperazone].

The effects of cefbuperazone (CBPZ) on the morphology of Escherichia coli in the urine of 10 patients with acute simple cystitis were studied by differential interference contrast microscopy and electron microscopy. The urine specimens were collected via catheter before, 5, 10, 15 and 30 minutes after intravenous administration of 0.25 g CBPZ. The minimum inhibitory concentrations for 8 Escherichia coli strains isolated from 8 patients against CBPZ were from 0.05 to 0.2 mcg/ml except one strain. The mean urinary concentrations at 5, 10, 15 and 30 minutes after administration of CBPZ were 274.8, 1181.5, 1343.4 and 931.5 mcg/ml, respectively. Urinalysis improved within 30 minutes after the administration. Filamentous cells of Escherichia coli were observed until 30 minutes after the administration. They had vacuole-like, and spheroplast and bulge formation were also observed in tested cases. CBPZ is suggested to have a high affinity for penicillin-binding protein (PBP) 3 and moderate affinity for PBP 1b and PBP 1a.

Acute Disease↗
PubMed1986

Morphological studies of Escherichia coli in the urine of patients with acute simple cystitis treated with aztreonam.

The effects of aztreonam (AZT) on the morphology of Escherichia coli in the urine of 5 patients with acute simple cystitis were studied by differential interference contrast microscopy. The urine specimens were collected via catheter before, 5, 15 and 30 minutes after intravenous administration of 1.0 gm AZT. The minimum inhibitory concentration of E. coli against AZT was 0.05 microgram/ml. The mean urinary concentrations at 5, 15 and 30 minutes after administration of AZT were 481.6 micrograms/ml, 1168.2 micrograms/ml and 993.4 micrograms/ml, respectively. In 4 patients, the urinalysis became normal within 30 minutes after the administration. Filamentous cells were observed at low and high urinary concentrations of AZT. They had vacuole-like formations. These findings were characteristic morphological changes caused by AZT. In the other patient, spheroplasts and ovoid cells were observed. AZT in the urine of patients with acute simple cystitis is suggested to have a high affinity for penicillin-binding protein (PBP) 3 and moderate affinity for PBP 1a.

Acute Disease↗
PubMed1983

[On the clinical effects of cefotiam on acute cystitis and changes in form of E. coli in the urine].

Cefotiam (CTM) was administered to female patients suffering from acute cystitis to examine its clinical efficacy and changes in form of the bacteria in urine. CTM was administered to 15 female patients with acute cystitis once a day for three consecutive days (1 g/time in 13 cases, and 0.5 g/time in 2 cases). The overall clinical efficacy was 100%. Bacteriological examination, revealed 13 cases of single infection by E. coli, and one case of E. coli and S. epidermidis (MIC: 0.78 micrograms/ml). MIC of E. coli ranged from 0.05 to 0.78 micrograms/ml. All of the strains were eradicated, the eradication efficacy being 100%. The forms of bacteria in the urine after the administration of CTM were examined in 8 cases (all due to E. coli). Bacteria in urine disappeared or formed a spherical form 5 minutes after the administration of CTM. Disappearance of E. coli was observed in 7 cases at 15 or 30 minutes after drug administration, and with the disappearance, improvements were also observed in the urinary findings. Of these cases, the urinary concentration of CTM was measured in 4 cases. It ranged from 296 to 6,893 micrograms/ml at 5 minutes after the administration. In one case of deficient excretion in urine, filamentation was observed, and with the rise in CTM concentration in urine, granular changes were observed in the shape of bacteria. CTM's excellent clinical efficacy was substantiated by the changes in the form of bacteria in urine.

Acute Disease↗