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Biomedical subjects

K Ineichen

Publications and source records attributed to K Ineichen.

6 recordsLinked to original sources
PubMed1981

The DNA sequence of the phage lambda genome between PL and the gene bet.

We have determined 3,400 base pairs of DNA sequence from the phage gamma genome which starts to the right of PL and runs to the left into the gene bet. The sequence thus includes the genes, N, ral, Ea10, cIII, kil and gam, as well as the transcription terminators TL1 and TL2. One surprising feature of the sequence is the presence in the region expected to be occupied by ral of a long open reading frame that, if it is expressed, would have to be transcribed from left to right, or counter to transcription from PL.

Bacteriophage lambda↗
PubMed1980

An unusual RNA polymerase binding site in the immunity region of phage lambda.

RNA polymerase binds very tightly at a site called Brex in the lambda immunity region, to the left of the rex gene and about 600 nucleotides to the right of PL. The complex formed is resistant to 1 M NaCl in the absence of nucleotide triphosphate. While in vitro little or no transcription is observed from Brex, in vivo, when inserted in a plasmid vector which allows detection of its activity, it acts as an efficient promoter. We have mapped the site protected by RNA polymerase against DNase and determined its sequence which is abnormal compared that of an average promoter.

Bacteriophage lambda↗
PubMed1980

The DNA sequence recognised by BglI.

The restriction enzyme BglI recognizes the DNA sequence: (Formula: see text) and cleaves it in the position shown by the arrows to leave 3' single-stranded protrusions three bases long.

Bacillus↗
PubMed1976

Lambda repressor regulates the switch between PR and Prm promoters.

The DNA region containing the Or operator, Pr and Prm promoters and their transcription initiations is sequenced. By binding to Or, repressor turns off Pr, turns on Prm and at higher concentrations turns off Prm, regulating its own synthesis. Prm mRNA is unique in beginning immediately with the initiation of translation, without a preceding leader sequence.

Base Sequence↗