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Biomedical subjects

K Inaba

Publications and source records attributed to K Inaba.

At least 253 records · Page 14Linked to original sources
PubMed1982

The effects of echinatin and its related compounds on the mitochondrial energy transfer reaction.

To investigate the mechanism by which various biological action of licorice root are brought about, the effects of echinatin as a small constituent of Glycyrrhiza echinata and several related compounds on mitochondrial energy transfer reactions were examined. The results obtained were as follows: 1) Echinatin, 4'-hydroxychalcone, chalcone and 3,4'-dihydroxychalcone at a low concentration cause deterioration of respiratory control and oxidative phosphorylation of isolated rat liver mitochondria. 2) Chalcone and 4'-hydroxychalcone stimulate both latent and DNP-ATPase activity of mitochondria. Echinatin inhibits DNP-ATPase activity while stimulating range latent ATPase activity in the low concentration. 3) Chalcone and 4'-hydroxychalcone induce a rapid potassium release from mitochondrial vesicles, while echinatin and 3,4'-dihydroxychalcone have lesser effect than the former two substances. From these results, it can be concluded that echinatin and several related compounds disturb the mitochondrial energy transfer reactions and membrane permeability.

Adenosine Triphosphatases↗
PubMed1982

Ontogeny of 'macrophage' function. III. Manifestation of high accessory cell activity for primary antibody response by Ia+ functional cells in newborn mouse spleen in collaboration with Ia- macrophages.

The ontogenesis of the responsiveness of murine whole spleen cells in the in vitro primary antibody response paralleled not only the development of competent lymphoid cells but also that of the accessory cell (A-cell) activity of spleen adherent cells (SAC). The Ia+ cell content of SAC (and also peritoneal exudate cells) was very low until 2 weeks of age. The phagocytic activity of macrophages in SAC was higher in newborns than in adults, though no significant difference was observed between Ia- and Ia+ macrophages in phagocytic activity. We attempted to reveal a high A-cell activity using cells in newborn spleen by means of our experimental strategy documented previously in adult mice (Inaba, Nakano & Muramatsu, 1981): though neither Ia- macrophage population (Ia- SAC) nor a temporarily adherent spleen cell population containing few phagocytic macrophages (crude non-macrophage cell fraction, CF) serves as an autonomous A-cell source, the collaboration of Ia+ non-macrophage cells in CF with Ia- SAC causes the manifestation of A-cell activity. Adult CF collaborated as well with newborn SAC as with adult Ia- SAC, indicating that newborn Ia- macrophages are functionally comparable with adult Ia- macrophages in the ability to collaborate with Ia+ non-macrophage cells. On the other hand, a high A-cell activity was generated by the combination of adult Ia- SAC with a large number of newborn CF cells, indicating that there exist competent Ia+ cells in newborn spleen, though much fewer than in adult spleen.

Animals↗
PubMed1981

Effects of L-glutamate on cyclic AMP levels in slices from different areas of rat cerebral cortex with a chronic iron-induced focus.

The effect of L-glutamate of cyclic AMP levels in different areas of rat cerebral cortex was examined during the development of an iron-induced focus in the left sensorimotor cortex. The addition of L-glutamate resulted in increased cyclic AMP levels in slices from the quarter of cortex with the iron-induced focus. The levels lowered in slices from the quarter of cortex most remote from the focus. Thus L-glutamate showed stimulatory and inhibitory effects on the accumulation of cyclic AMP.

Animals↗
PubMed1981

Cellular synergy in the manifestation of accessory cell activity for in vitro antibody response.

The accessory cell (A-cell) activity of murine splenic adherent cells (SAC) and peritoneal exudate cells (PEC) for in vitro anti-SRBC and anti-DNP-KLH antibody responses of spleen lymphoid cells was abolished by the depletion of Ia+ cells from SAC and PEC by treatment with anti-Ia alloantiserum plus complement. The surviving Ia- macrophages in SAC and PEC were incompetent to achieve the A-cell activity. We prepared a crude and a further purified nonmacrophage cell fraction (termed CF and NMF). Nearly 70% of CF cells and more than 90% of NMF cells were Ia+ cells. The mature macrophage content of CF was less than 2%, and that of NMF was virtually negligible; suspected dendritic cells were contained at 5 to 10% in CF and 20 to 30% in NMF. The majority of cells in CF and NMF were lymphoid cells. This offered no problem in these experiments, since CF and NMF were added to lymphoid cells, in order to investigate the A-cell activity, usually in a ratio of 1 to 100. Neither CF nor NMF was adequate by itself to manifest the A-cell activity. However, the combination of either CF or NMF with Ia- macrophages resulted in the development of high A-cell activity. This did not occur with use of CF depleted of Ia+ cells or of NMF treated with anti-Ia without complement. These results indicated that the synergy between Ia- macrophages and Ia+ cells, most probably Ia+ nonmacrophage cells, was effective in developing the A-cell activity. It was also found that the interaction between the lymphoid cell and the Ia+ participant in the A-cell activity was genetically restricted, but Ia- macrophages functioned across the H-2 barrier.

Animals↗
PubMed1979

Improvements of dissolution characteristics and chemical stability of 16,16-dimethyl-trans-delta 2-prostaglandin E1 methyl ester by cyclodextrin complexation.

Inclusion complexation of 16,16-dimethyl-trans-delta 2-prostaglandin E1 methyl ester (I), which is effective in early pregnancy termination, with cyclodextrins in water was ascertained by a solubility study. A solid complex of I-beta-cyclodextrin in a 1:2 molar ratio was obtained, and its dissolution behavior and chemical stability were examined. The results indicated that the complex may have great utility as a rapidly dissolving form of I with prolonged storage time.

Biopharmaceutics↗
PubMed1979

Heat stable cell growth inhibiting factor isolated from rat liver microsomes.

A heat stable cell growth inhibiting factor was isolated from rat liver microsomes by hot salt extraction, ethanol fractionation and the hot phenol method. The factor was contained in the RNA fraction (designated as mhRNA). mhRNA inhibited the growth of mouse fibroblast (L-929) cells at a relatively low concentration (55 microgram/ml of culture medium). The molecular weight of mhRNA was about 27,000 and the base composition was guanine and cytosine rich.

Animals↗
PubMed1978

Fractionation and characterization of euchromatin isolated from mouse ascites sarcoma cells.

Euchromatin specimen prepared by the usual method formed large clumps and had various shapes under electron microscopy. A method of separation of the euchromatin specimen into chromatin fractions having relatively homogeneous form was examined and partial characterization of these fractions was carried out. The heavy euchromatin fraction was a large network of thin fibrils (about 100 A in diameter) and various thick fibers. The intermediate euchromatin fraction consisted of relatively homogeneous networks of thick knobby fibers (about 250 A in diameter). The light euchromatin fraction had metworks of thick fibers. These chromatin fractions were quantitatively prepared from sonicated nuclei of mouse ascites sarcoma cells. Twenty-one or twenty-two bands of non-histone proteins besides histones were detected in these chromatin fractions by SDS-polyacrylamide gel electrophoresis. There were significant differences in the electrophoretic patterns of non-histone proteins among these chromatin fractions.

Animals↗
PubMed1977

Utilization of cyclodextrin complexation for separation of E, A, and B prostaglandins by ion-exchange liquid chromatography.

Application of cyclodextrin complexation to the separation of E-, A-, and B-type prostaglandins by ion-exchange liquid chromatography is demonstrated. The addition of alpha-or beta-cyclodextrin into the mobile phase on an anion-exchange support decreased the retention times of the prostaglandins significantly because of soluble complex formation. Chromatographic separation behavior is discussed on the basis of the stability of the inclusion complex. A rapid and sensitive method for the separation and quantification of the prostaglandins, using beta-cyclodextrin in the mobile phase, is described.

Chemical Phenomena↗
PubMed1977

Cell-mediated and humoral immune responses in mice. II. Sensitizing conditions for delayed-type hypersensitivity.

Conditions for the induction of delayed-type hypersensitivity (DTH) in mice to serum proteins ware studied. DTH to bovine serum albumin (BSA) was induced by a subcutaneous immunizaiton with the antigen emulsified with Freund's complete adjuvant. The level of DTH was found to be dempendent not only on the dose of antigen but also on the amount of mycobacteria included in the adjuvant. DTH to other serum proteins was also induced under the same sensitizing conditions as for BSA. Success in the adoptive transfer with lymphoie cells and suppressive effect of anti-thymocyte serum indicated that delayed-type reaction studied here was the manifestation of cell-mediated immunity.

Adjuvants, Immunologic↗
PubMed1977

RNA synthesis in mitochondria isolated from rat liver.

Mitochondrial RNA (mtRNA) was synthesized from purine and pyrimidine nucleosides in coupling with oxidative phosphorylation using isolated mitochondria. The in vivo synthesized mtRNA was adenine-uracil rich and sedimented at about 20 S by sucrose density gradient centrifugation. A major part of the newly synthesized mtRNA was shown to be poly (A)-containing RNA by the resistance to the digestion with pancreatic RNase and RNase T1 and the affinity to poly (U)-Sepharose columns or Millipore filters.

Animals↗
PubMed1977

Purification and some characteristics of liver cytosol cornin, an antimitotic substance from rat liver cytosol.

Further purification and characterization are reported on rat cytosol cornin (RLCC), an antimitotic substance. Fraction I (purified RLCC) was purified more than 10-fold from crude RLCC with Sephadex G-50 column chromatography and showed a remarkable inhibitory effect on division of inseminated sea urchin eggs and mouse fibroblast cells. Fraction I was observed as one spot, and the molecular weight was estimated to be about 25,000 by thin layer gel filtration. Fraction I contained protein (92%) and RNA (8%), but the antimitotic activity was scarcely affected by treatment by pancreatic RNase. The protein of Fraction I was separated into two bands by SDS-polyacrylamide gel electrophoresis, and the molecular weight was estimated as 10,000 and 15,000, respectively. The 50% inhibition dose of Fraction I on the first division of inseminated sea urchin eggs and on proliferation of mouse L cells was about 2.5 X 10(-5) g/ml and 5 X 10(-4) g/ml, respectively. The yield of fraction I was about 35 mg from 100 g rat liver.

Amino Acids↗