Search PubMed⌕ Search

Biomedical subjects

K I Welsh

Publications and source records attributed to K I Welsh.

At least 253 records · Page 14Linked to original sources
PubMed1976

Preparation of antisera specific for human B cells by immunization of rabbits with immune complexes.

Three rabbit antisera are described which are specific without absorption (titer 1:100) for separated human B cells, as measured by complement and non-complement fixing assays. The method of production of these sera involved injections of rabbits with precipitin lines formed between 10mul of three separate detergent solubilized membrane preparations and 4mul aliquots of rabbit antisera to human B cells. In addition to being B cell specific, the three sera block the MLC reaction, inhibit aggregated IgG binding to B cells, and show differential degrees of B cell lysis when tested on a panel of separated B and T cells. These and other properties suggest that the target specificities of the antibodies are the human equivalent of the murine Ia antigens.

Animals↗
PubMed1975

A radioimmunoassay of cellular surface antigens on living cells using iodinated soluble protein A from Staphylococcus aureus.

Soluble protein A from Staphylococcus aureus does carry great promise as a marker for cellular surface determinants, due to its specific reaction with, and high affinity for, most subclasses of mammalian IgG. In this article we present the different parameters involved in a radioimmunoassay using 125-I-labelled protein A. Using such an approach the actual technical procedures involved are reported in detail together with tests for mammalian alloantigens, including HL-A in the human, H-2 in the mouse and AgB antigenic sites in the rat, as this presents an unique opportunity to compare them with already widely used assays for transplantation antigens. The different parameters of the assay are analysed in view of measuring with precision quantities of cell-surface IgG molecules, thereby allowing possible determinations of antigenic site numbers in a new and simplified manner.

Animals↗