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Biomedical subjects

K Horn

Publications and source records attributed to K Horn.

At least 91 records · Page 5Linked to original sources
PubMed1987

Immunoturbidimetric assay for the determination of microalbuminuria using the Hitachi analyser.

A sensitive and specific immunoturbidimetric method is described for the determination of low concentrations of urinary albumin using a Hitachi 704 or 705 analyser. The sensitivity was 1 mg/l and the precision attained was good (CV 15%, 7% and 6% for low, medium and higher albumin concentrations). The assay was used to determine urinary albumin excretion rates in healthy controls (less than 15 micrograms/min) and in type I diabetics. Since microalbuminuria (30-200 micrograms/min) seems to be a good predictor for the development of diabetic nephropathy and other late diabetic complications, this assay is suitable for the necessary screening and follow-up of diabetic nephropathy. In contrast to RIA methods, no radioactive tracers are needed.

Adolescent↗
PubMed1987

Hypertrophy and hyperplasia during goitre growth and involution in rats--separate bioeffects of TSH and iodine.

Goitre growth was investigated in rats receiving a low iodine diet (less than 0.1 microgram iodine/g) and either 1 g/l KClO4 or 1 g/l propylthiouracil (PTU), or a combination of KClO4 or PTU with 50.82 nmol/1 T3 in tap water for 3 weeks. To investigate goitre involution, rats with iodine-deficient goitres were treated for 3 weeks either with T3 (0.5 microgram T3/day = 0.768 nmol/day), iodide (0.5 or 2.7 micrograms KI/day) or a combination of T3 with both iodide doses. Histology together with total DNA distinguished between hypertrophy and hyperplasia of the gland. During goitre growth there was highly significant correlation between goitre weight and TSH serum level (r = 0.93, P less than 0.001). Thyroid total DNA, however, was only weakly correlated to TSH but was inversely related to the degree of iodine deficiency. During goitre regression, TSH levels were normalized, histological signs of hypertrophy had disappeared, and thyroid weight was nearly normalized in all therapy groups. Total DNA, however, was normalized only with 2.7 micrograms KI/day (95 +/- 18 micrograms DNA/gland), and still elevated in all other groups. The highest DNA levels were found under T3 therapy (143 +/- 21 micrograms DNA/gland) and under 0.5 microgram KI/day (161 +/- 19 micrograms DNA/gland). Reduction of total DNA was independent of TSH, but followed replenishment of the iodine content of the glands. We conclude that TSH mainly induces hypertrophy, whereas thyroid hyperplasia is mainly regulated by the intracellular iodine content.

Animals↗
PubMed1987

Relation of attention deficit and conduct disorder to vigilance and reading lag.

The relationship between DSM-III Axis I diagnoses 'attention deficit disorder with hyperactivity' (ADDH), 'conduct disorder' (CD) and 'anxiety disorder' (AD) and measures of attention and reading were studied in 158 children. Children diagnosed as having severe or moderate ADDH were found to be younger at referral and to have a lower IQ than were children with CD and AD. When age, IQ, social class and sex were controlled, children with severe ADDH were found to perform significantly worse than other diagnostic groups on some tests of vigilance and reading age. The data suggest that children with severe ADDH form a distinct group, and those with mild ADDH overlap symptomatically and on tests of vigilance with children with CD.

Achievement↗
PubMed1986

[Results of a multicenter evaluation of enzyme immunoassays for thyrotropin (TSH)].

This paper summarises the results of the evaluation of an enzyme immunoassay for thyrotropin by a group of 17 laboratories. This sandwich enzyme immunoassay is based on the specific binding of the beta-subunit of thyrotropin by monoclonal antibodies coated on polystyrene tubes. Thyrotropin, bound to the tube wall, is determined with horse radish peroxidase coupled to the Fab fragment of a polyclonal sheep antibody against the alpha-subunit of thyrotropin. The lower limit of detection of 0.5 mU/1 was adequate, and the precision of the enzyme immunoassay was comparable with that found in radioimmunoassays for thyrotropin. The intraassay CV was in the range of 2 to 10% for a serum containing 1.5 to 5 mU/1 thyrotropin. The interassay CV was in the range of 5 to 10% for a serum containing about 6 mU/1. The recovery of thyrotropin standards in human thyrotropin-free serum was between 93% and 101%. Cross-reactions with human choriongonadotropin and gonadotropins were excluded. There were no interferences in haemolytic and lipaemic samples or by high bilirubin concentrations. Comparison of thyrotropin levels measured with different radioimmunoassays and with the enzyme immunoassay gave correlation coefficients in the range of 0.925 and 0.995. Advantages of the enzyme immunoassay are the absence of radioactive substances and the short incubation period of 3 hours. Incubation overnight results in a higher sensitivity of 0.18 mU/1. This assay can therefore be regarded as one of the new highly sensitive thyrotropin assays (Bernutz, C. et al. (1985) Clin. Chem. 31, 289-292).

Antibodies, Monoclonal↗