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Biomedical subjects

K Horii

Publications and source records attributed to K Horii.

At least 37 records · Page 2Linked to original sources
PubMed1993

Application of in vitro bromodeoxyuridine labeling of oral cancers.

We used immunohistochemical methods to investigate whether in vitro labeling with bromodeoxyuridine (BrdU) of oral cancer tissues is useful in pathological diagnosis. BrdU-positive cells were found around the basal region of the epithelium in normal oral mucosa, in a papilloma specimen and in tissue with moderate dysplasia. The labeling rates (percentage of BrdU-positive cells, LR) for these cases were 1.2%, 6.9% and 7.5%, respectively. In well-differentiated carcinoma, more layers of BrdU positive cells were observed from the basal layer toward the surface. The LR in this region was 13.9-17.0%. In vitro BrdU labeling of oral tumors may be useful in pathological diagnosis, since the LR is lowest in normal tissues, higher in benign tumors, and highest in malignant tumor tissues.

Bromodeoxyuridine↗
PubMed1993

[Benefits of a community oriented fluoride mouth rinsing program].

The benefits of a school-based fluoride mouth rinsing in a community dental health program were evaluated. In village "M", children from 4 to 14 years old have been participating in a school-based fluoride mouth rinsing program since 1974. In addition, an informal association for dental health of children was inaugurated in 1975 to support the community dental health program. In village "K", there were no fluoride mouth rinsing programs. However, the usual dental health education conducted in all schools was similar in both communities. In order to compare these two communities, dental examinations were carried out in 1990. The total number of subjects from 6 to 14 years old was 331 in village "M" and 408 in village "K". In village "M" for longitudinal comparison, 12-year-olds, 1,054 in total, were examined annually from 1974 to 1990. The DMFT-index in all subjects in village "M" was 76.1% lower than in village "K". In village "M", the DMFT-index in 1990 decreased 79.0% compared to 1974. The economic benefits obtained through the caries reduction were evaluated. In 1990 the mean cost of dental treatment for subjects 14 years old was 27,840 yen per person in village "K" and 4,248 yen in village "M". The cost-benefit ratio was 18.8 and the cost-effectiveness ratio was 137 yen. These remarkable benefits from the prevention of dental caries appears to have been mainly obtained due to the participation of children aged 4 to 14 years old in a school-based fluoride mouth rinsing program.

Adolescent↗
PubMed1991

[Two brother cases of late-onset familial amyloidotic polyneuropathy in Kyoto].

Measurement of variant Met30 transthyretin is diagnostic for a patients with familial amyloidotic polyneuropathy (FAP) type I. The elder brother first noticed numbness of the feet at 64 years of age, and developed weakness of the legs. A few years later, he noticed numbness of the hands, and he was admitted to the hospital at 67 years of age. He was emaciated and had hoarseness and macroglossia. He had moderate muscle atrophy and weakness of all extremities with distal predominance. Deep tendon reflexes were hypoactive in the upper limbs and absent in the lower limbs. There was marked sensory loss of pain and temperature in all 4 limbs distally, and position sense was also impaired. He had mild orthostatic hypotension, severe cardiomegaly and arrhythmia. The younger brother noticed cold sensation of the feet and sexual impotence at 59 years of age. Two years later, he had numbness of the feet and developed weakness of the legs. At 65 years of age, he was admitted to the hospital because of the micturition syncope. He was emaciated and had macroglossia. He had moderate muscle atrophy and weakness of all extremities with distal predominance. Deep tendon reflexes were absent. There was marked sensory loss in the extremities which was predominant in pain and temperature. He had severe orthostatic hypotension (112/70 mmHg in supine position, 50/30 mmHg on standing). Plasma NE value was low and showed poor response to standing. He had neither cardiomegaly nor arrhythmia. Their parents were supposed to have no neurological symptom and were not related with any other Japanese foci of FAP.(ABSTRACT TRUNCATED AT 250 WORDS)

Aged↗
PubMed1991

[A case of phenytoin intoxication induced by hypothyroidism].

A 42-year-old woman who presented phenytoin intoxication induced by acute hypothyroidism was reported. She had a 29-year history of hypothyroidism and a 18-year history of epilepsy. She was treated with phenytoin (PHT) 100 mg, mephobarbital (MPB) 200 mg, valproic acid (VPA) 400 mg and thyroid powder 100 mg daily for 2 years. She had no medical problem until she noticed gait disturbance and diplopia which appeared 1 month after sudden withdrawal of thyroid powder. On admission, she was somnolent and somewhat disoriented. She had nystagmus in horizontal direction of gaze. Her speech was slurred and she could not sit nor stand due to trunkal ataxia. There was prominent intentional tremor in finger-nose test and heel-shin test showed severe ataxia. Blood cell count and blood chemistry examinations were normal. Serum PHT, phenobarbital (PB) levels were elevated as to 26.4, 36.4 micrograms/ml, respectively. VPA level was low. The endocrinological examinations revealed primary hypothyroidism. EEG showed generalized slow background, but cranial MRI, EMG, SEP and ECG were normal. Thyroxine (T4) administration was started soon, and in the course of thyroid hormone replacement, her cerebellar symptoms gradually improved and serum PHT level decreased even to the subtherapeutical level with the same amount of antiepileptic drugs treatment. By the 40th day of admission, thyroid function became normal and cerebellar signs disappeared, however, she needed 200 mg PHT daily to obtain good control of epilepsy. Cerebellar symptoms of this patient were thought to be PHT intoxication rather than ataxia caused by hypothyroidism itself from the viewpoint of clinical manifestations.(ABSTRACT TRUNCATED AT 250 WORDS)

Acute Disease↗
PubMed1990

Effect of sodium fluoride on growth of human diploid cells in culture.

Cytotoxicity of sodium fluoride (NaF) on human diploid cells in exponential growth was investigated using a) Flow 1000 cells, passage No. 13, obtained from skin and muscle tissues of male black foetus, b) IMR-90 cells, passage No. 22, derived from lung tissue of female Caucasian foetus and c) primary fibroblast-like cell cultures from 5 Japanese whole foetuses. Diploid cells did not survive at 20 p.p.m. of ionic fluoride (F-) concentration. However, the cells were capable of proliferation with no significant impairment of growth up to 0.2 p.p.m. F-, a level which is much higher than the plasma concentration in human subjects from areas with highly fluoridated water. The growth of the cells was markedly affected by F- concentrations greater than 2 p.p.m.

Aneuploidy↗
PubMed1989

[Examination and treatment of dental caries in the Niigata Prefectural Institute of Total Training for the Mentally Retarded, colony Niigata Shiroiwa-no-Sato].

The purpose of this study was to learn about the prevalence of dental caries in mentally retarded individuals and to follow up the treatment of the caries. The study group comprised 144 severely retarded individuals (79 males and 65 females) aged 6-19 at the time of their admission to the Institute. Following results were obtained by analyses of their dental examination over a period of records in 15 years from 1973 to 1987 and of their treatment charts over a period of 8 years from 1980 to 1987. 1. The DMFT index at the time of their admission showed a tendency to increase gradually with age. In the DMFT index by age, there was no significant difference between that of the Institute and that of the Survey of Dental Disease by Ministry of Health and Welfare Japan in 1981. 2. The FT index was lower and the MT index was higher than the indices of the Survey, and each of differences showed a statistical significance for almost of all ages. Also in the proportion of both indices to the DMFT index, there were significant differences respectively. 3. During the 8 years between 1980 and 1987, 740 teeth were treated with 388 amalgam fillings (52.4%), 174 resin fillings (23.5%), 22 metal crowns (3.0%), 55 endodontic therapies (7.4%) and 101 extractions (13.7%). 4. After Our department started the treatment program in 1980, there were under 0.5 untreated teeth in each individual for each year. Moreover, missing teeth increased slightly in individuals who were on the register more than 2 years. 5. One of the 237 filled teeth (0.4%) and 2 of 21 endodontically treated teeth (9.5%) were extracted because of unfavorable prognosis during 8 years of the follow-up survey period.

Adolescent↗
PubMed1989

[A case of miliary tuberculosis associated with ARDS, DIC and bilateral pneumothorax].

We reported a case of 64 a year-old male patient of miliary tuberculosis associated with ARDS, DIC and pneumothorax, who had a history of gastric ulcer and pulmonary tuberculosis. On admission his chief complaints were fever, fatigue, palpitation, appetite loss and weight loss, and most noticeable abnormalities were bleeding from the gastric ulcer and miliary shadow on the chest x-ray film with hypoxemia. On the day after admission to the hospital he was diagnosed as ARDS as he showed severe hypoxemia due to extensive tuberculous infiltration in bilateral lung fields, and treatment with antituberculous drugs and steroids were started. On the third hospital day DIC appeared on laboratory data, Gabexate mesilate (FOY) for DIC and respirator for ARDS were introduced. Two weeks later pulmonary infiltration, PaO2 and general condition were somewhat improved. On the 15th day after admission pneumothorax occurred on the right side, and on the 20th day on the left. Tube drainage of both pleural cavities, and instillation of OK-432 and Fibrinogen HT into the right pleural cavity were done, but it showed no effect. Two months after admission pouring Fibrinogen HT and thrombin into the left B1+2 and right B1 with cannula washing pipe through the instrument channel of bronchoscope was carried out. A few days later air leakage stopped and collapsed lungs were completely expanded. This method is effective in the case of incurable pneumothorax with pulmonary hypofunction.

Disseminated Intravascular Coagulation↗
PubMed1984

Isolated ACTH deficiency associated with Hashimoto's thyroiditis: report of a case.

A 65-year-old female with general malaise, anorexia and marked emaciation was studied by secretion stimulation tests on 6 anterior pituitary hormones. Only ACTH showed no response and the other 5 hormones responded normally. The basal value of antidiuretic hormone was normal. She was found to be suffered from primary hypothyroidism. Though neither antithyroid antibodies nor other autoantibodies were found, Hashimoto's thyroiditis was confirmed by a thyroid open biopsy. Neoplastic lesions of the hypophysis were ruled out by various X-ray and CT examinations of the sella turcica as well as the brain. The case was concluded to be isolated ACTH deficiency associated with Hashimoto's thyroiditis. The substitution therapy using small doses of adrenocortical hormone and thyroid hormone has kept her well and she has been living normally for these five years.

Adrenocorticotropic Hormone↗
PubMed1982

Comparison of transcription of beta-lactamase genes specified by various ampicillin transposons.

The beta-lactamase gene from four kinds of ampicillin transposons, Tn2601, Tn3, Tn2602 and Tn1, specifying the type I (or TEM type, alternatively) beta-lactamase was cloned onto plasmid pACYC184, and the level of in vivo transcription from each beta-lactamase gene was determined by DNA-RNA hybridization. Type I beta-lactamase is very uniform enzymologically, but heterogeneous in absolute levels of enzyme activity. The results demonstrated that the heterogeneity can be explained by the efficiency of transcription of each beta-lactamase gene, suggesting a difference in its promoter efficiency. A comparison of the levels of transcription of the beta-lactamase gene and the whole ampicillin transposon suggested that the beta-lactamase gene has the strongest promoter all of the genes in the ampicillin transposon.

Ampicillin↗