Search PubMed⌕ Search

Biomedical subjects

K Herrmann

Publications and source records attributed to K Herrmann.

At least 109 records · Page 6Linked to original sources
PubMed1982

Acid lysosomal hydrolases in systemic sclerosis and other connective tissue diseases.

The activities of five lysosomal hydrolases were determined fluorometrically in the serum of patients with systemic sclerosis (PSS), systemic lupus erythematosus (SLE), dermatomyositis (DM), rheumatoid arthritis (RA), or Raynaud's disease (RD). In PSS the beta-galactosidase activity was significantly increased compared with controls and the other connective tissue diseases. The beta-N-acetyl-glucosaminidase was significantly increased in PSS, SLE and DM. In PSS both enzymes were more active in the early stage of the disease than later. These changes of enzyme pattern seem to be a relatively reliable marker for the differential diagnosis of PSS compared to other connective tissue diseases, especially for RD, in which the beta-galactosidase activity was significantly decreased. Further work is required to determine whether these polysaccharide-degrading acid hydrolases play a role in the pathogenesis of PSS.

Acetylglucosaminidase↗
PubMed1981

Role of virus strain in conventional and enhanced measles plaque neutralization test.

A low-passage Edmonston strain of measles virus gave on the average four-fold higher antibody titers than two high-passage strains of Edmonston virus when used in a virus plaque neutralization test. Heterologous anti-human immunoglobulin did not affect antibody titers obtained with the low-passage virus but enhanced antibody titers obtained with the high-passage viruses on the average eight-fold. These differences in virus sensitivity to neutralization and neutralization potentiation by anti-immunoglobulin were interpreted as being possibly due to differences in density or accessibility of the functional antigens of measles virus. The conventional plaque neutralization test utilizing the low-passage Edmonston virus was about 10 times more sensitive than the virus CPE-neutralization test, 60 times more sensitive than the measles hemagglutination-inhibition test and 220 times more sensitive than the measles complement-fixation antibody test.

Animals↗
PubMed1981

[Alpha 1-antitrypsin: production and binding of in vitro stimulated peripheral blood lymphocytes].

Three day cultures of human peripheral blood lymphocytes (PBL) contain significant numbers of lymphoblasts binding 125I-alpha 1-AT as well as those with membrane-associated alpha 1-AT detectable by immunofluorescence. Co-cultivation of PBL with T cell mitogens leads to the appearance of lymphoblasts with membrane-associated alpha 1-AT while B cell mitogens induce lymphoblasts with a binding capacity for 125I-alpha 1-AT. It could be shown that unstimulated lymphocytes neither have membrane-associated alpha 1-AT nor possess a binding capacity for it. The highest number of lymphoblasts with membrane-associated alpha 1-AT was found in Con A stimulated cultures as revealed by indirect immunofluorescence. By contrast, PHA or PPD stimulated cultures contained much less such cells of lower fluorescence intensity. Co-cultivation of PBL with the B cell mitogens LPS and rabbit-anti-human gammaglobulin-IgG-F(ab')2 induced lymphoblasts with a clear binding capacity for 125I-alpha 1-AT, while dextran-sulphate had no effect. A possible regulative role of alpha 1-AT in the immune response is discussed.

Humans↗
PubMed1980

Detection of basement membrane zone antigens and C3 fragments in the blister fluid of bullous pemphigoid.

In polyacrylamide gel gradient electrophoresis, serum and blister fluid of patients with bullous pemphigoid showed a nearly identical protein distribution pattern except for one more concentrated fraction in the blister fluid. This fraction could be isolated by several gel chromatographic steps and then be characterized as a protein with a molecular weight of 240,000 (BFP 240,000) consisting of several subunits. Two of these subunits could be identified as C3 fragments and two other ones as basement membrane zone antigens (BMZ-Ag) by means of a modified Laurell technique. The molecular weight of the BFP 240,000 may be small enough to allow it to penetrate through the vessel wall into the blood circulation where, with the BMZ-antibodies (BMZ-Ag-BMZ-Ab-complexes can be formed. The occurrence of such immune complexes in the serum has been shown in a previous paper.

Antigens↗