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Biomedical subjects

K Heilmann

Publications and source records attributed to K Heilmann.

At least 19 recordsLinked to original sources
PubMed2004

The influence of the chemical composition of cell culture material on the growth and antibody production of hybridoma cells.

The multiplication and antibody production of murine hybridoma cells cultured on five different polymer membranes were tested and compared with conventional tissue culture polystyrene (TCPS). Membranes were prepared from polyacrylonitrile (PAN) and acrylonitrile copolymerized with N-vinylpyrrolidone (NVP20, NVP30), Na-methallylsulfonate (NaMAS) and N-(3-amino-propyl-methacrylamide-hydrochloride) (APMA). Cell number and antibody concentration were quantified as criteria for viability and productivity. Adhesion of hybridoma cells was characterized by vital and scanning electron microscopy. The results suggest that a strong adhesion of cells, observed on APMA and TCPS, increased cell growth but reduced monoclonal antibody production. In contrast membranes with lowered adhesivity such as NVP20 provided favourable conditions for monoclonal antibody production. In addition it was shown that this membrane also possessed a minor fouling as indicated by the low decrease of water flux across the membrane after protein adsorption. It was concluded that NVP20 could be a suitable material for the development of hollow fibre membranes for bioreactors.

Animals↗
PubMed1997

[Hyperplastic polyps of the colon. An inhomogeneous group of pathologic-anatomic changes].

A total of 334 hyperplastic polyps of the colon of 266 patients were examined under epidemiologically, morphometrically and histochemically. Of these, 62 cases (23%) were associated with adenomas, 18 cases (7%) with carcinomas. Hyperplastic polyps are not entirely homogeneous, pathologically and -anatomically: Carcinoma-associated hyperplastic polyps are larger in diameter, the size and width of their crypts is greater, and they have a higher percentage of goblet cells with a higher amount of sialomucins as shown by HID/AB stain.

Adenomatous Polyps↗
PubMed1988

[Malignant hyperthermia. An unusual course of a rare disease].

An account is given of unusual course of a hyperthermic crisis in a 23-year-old male who underwent repeated anesthetics. As yet little has been reported about Isoflurane, which we presume to have been the triggering agent. In this case only the surgically untreated lower extremity developed rigor, with which malignant hyperthermia is associated, whereas the surgically treated extremity, where circulation had been stopped with a tourniquet, remained unaffected. Rigor and contracture of the affected extremity were so severe that they led to a compartment syndrome, necessitating fasciotomy. No observation of this kind has been published before. In addition to a discussion of this dissociated effect in malignant hyperthermia, a detailed account of the course of the crisis is given.

Adult↗
PubMed1986

[Therapy and prevention of reflux esophagitis. Results of a multicenter study with cimetidine. I: Epidemiology and results of acute therapy].

In a sequential observational study efficacy and safety of two dosage regimens of cimetidine for the treatment of reflux-oesophagitis (RE) were examined. 22 office-based specialists took part in the trial. 187 patients received 1600 mg cimetidine (400 mg q.i.d.) daily and 136 patients received 800 mg cimetidine (400 mg b.i.d.) daily, over twelve weeks. The two dosages proved to be equally effective. In the group of patients with RE of stage I at the beginning of the trial, the healing rates were 82% under 1600 mg cimetidine/die and 86% under 800 mg cimetidine/die. In the group of those patients, who entered the study with RE of stage II, in both dosage groups the lesions of 87% of the patients were healed completely or had decreased to stage I. The severity of the mucosal lesions was judged endoscopically, based on a classification scheme modified according to Savary and Miller. The incidence of unwanted symptoms was not significantly different in both dosage groups (1600 mg: 5.3%, 800 mg: 3.4%); none of the symptoms which occurred was judged as definitely caused by cimetidine. The patient collective of the trial was characterized epidemiologically; out of the risk factors examined, only duration of RE history and number of previous RE episodes correlated with the severity of the disease, smoking and alcohol consumption showed no influence.

Adolescent↗
PubMed1985

[A new cricothyreotomy set for emergencies].

Cardio respiratory emergencies in which intubation is by any reason not possible are rare. The traditional procedure of emergency tracheotomy can be complicated by tracheal esophageal perforation. There is now available a new emergency cricothyreotomy set "Nu-Trake" which is easy to handle even by the less experienced anaesthesiologist. As shown in our study the risk of tracheo esophageal perforation is very small with the new emergency coniotomy set. We believe the device should be part of the standard equipment of every emergency ambulance, after some improvements by the manufacturer.

Emergencies↗
PubMed1984

[Drugs as a risk factor].

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Drug-Related Side Effects and Adverse Reactions↗
PubMed1983

[Behavior of intraocular pressure during long-term treatment with a guanethidine-adrenaline combination].

Eight patients with chronic open-angle glaucoma treated regularly with different drugs (mainly timolol) and having a mean IOP of 20,4+/-1,2 mm Hg were subsequently treated for 9 months with Suprexon eye drops (guanethidine 1% + epinephrine 0,2%). At the end of the study the mean IOP was 17,6+/-1,3 mm Hg. Suprexon eye drops were found to have a long-lasting hypotensive effect. The straight-line regression test utilizing all the IOP measurements - 7 within the 9 months of the trial - showed no statistically significant tachyphylaxis (p less than 0.03). As a consequence no other drugs had to be added to control the IOP. Moreover, this treatment proved to be significantly more potent than timolol (p less than 0.05). While under treatment the patients suffered from conjunctival hyperemia and ocular pain, but these diminished in the course of time. No systemic side effects were observed.

Adult↗
PubMed1983

Innovations in drug delivery systems.

The history of pharmaceuticals has reflected a concern with the development of new chemical entities. Only recently has the importance of dosage form design been recognized as a vital component of effective therapy. Not only has the time and expense involved in the research of the new molecules escalated dramatically, but it has been discovered that many of these products might be improved through incorporation in rate-controlled dosage forms. Conventional formulations have provided vehicles with which to introduce agents into the body. New therapeutic systems offer great versatility since they permit localized treatment of specific tissues, sophisticated methods of animal experimentation, and controlled-release methods for systemic delivery. These new dosage forms are diverse, precise, and adaptable in many therapeutic situations. A membrane-controlled gastro-intestinal therapeutic (GIT) system resembling a conventional tablet in external appearance has recently been developed. This system is designed for the constant, controlled release over a period of 10 to 12 hours of drug in solution and uses osmotic activity as the source of energy. The GIT system, as used for 'Osmosin' (sodium indomethacin trihydrate), differs from conventional sustained-release and other controlled-release dosage forms in that the rate of release is not dependent upon pH to release the medication and is designed, therefore, to overcome the problems associated with unpredictable release patterns.

Animals↗
PubMed1978

[The intestinal and diffuse type of the gastric carcinoma (author's transl)].

According to the Laurén classification 357 carcinomas of the stomach were reclassified into intestinal and diffuse types. 58.2% were assigned to the intestinal type, 37.5% to the diffuse and 4% to a "mixed" group. There is obvious correlation between the intestinal type, the patients' advancing age and the incidence of chronic, atrophising gastritis with intestinal metaplasia. The diffuse type on the other hand occurs irrespective of gastric changes - mostly in younger persons. The intestinal type of gastric carcinoma frequently is found in risk areas where incidence is "epidemic" in nature; morphology and genetic origin seem to be gastric mucosa changes and intestinal metaplasia or dysplasia. Classifying gastric carcinoma according to Laurén (two groups) is also an appropriate method in our regions as was confirmed by the results we obtained; it is superior to the much more differentiated macro- and microscopic classifications used so far. Because of its simplicity, it is better suited for epidemiological and pathogenetic studies of the gastric carcinoma.

Aged↗