Biomedical subjects

K Hatakeyama

Publications and source records attributed to K Hatakeyama.

At least 109 records · Page 6Linked to original sources

PubMed2000

Highly divergent sequences of the pollen self-incompatibility (S) gene in class-I S haplotypes of Brassica campestris (syn. rapa) L.

Self-incompatibility (SI) enables flowering plants to discriminate between self- and non-self-pollen. In Brassica, SI is controlled by the highly polymorphic S locus. The recently identified male determinant, termed SP11 or SCR, is thought to be the ligand of S receptor kinase, the female determinant. To examine functional and evolutionary properties of SP11, we cloned 14 alleles from class-I S haplotypes of Brassica campestris and carried out sequence analyses. The sequences of mature SP11 proteins are highly divergent, except for the presence of conserved cysteines. The phylogenetic trees suggest possible co-evolution of the genes encoding the male and female determinants.

Alleles↗

PubMed2000

The S receptor kinase determines self-incompatibility in Brassica stigma.

The self-incompatibility possessed by Brassica is an intraspecific reproductive barrier by which the stigma rejects self-pollen but accepts non-self-pollen for fertilization. The molecular/biochemical bases of recognition and rejection have been intensively studied. Self-incompatibility in Brassica is sporophytically controlled by the polymorphic S locus. Two tightly linked polymorphic genes at the S locus, S receptor kinase gene (SRK) and S locus glycoprotein gene (SLG), are specifically expressed in the papillar cells of the stigma, and analyses of self-compatible lines of Brassica have suggested that together they control stigma function in self-incompatibility interactions. Here we show, by transforming self-incompatible plants of Brassica rapa with an SRK28 and an SLG28 transgene separately, that expression of SRK28 alone, but not SLG28 alone, conferred the ability to reject self (S28)-pollen on the transgenic plants. We also show that the ability of SRK28 to reject S28 pollen was enhanced by SLG28. We conclude that SRK alone determines S haplotype specificity of the stigma, and that SLG acts to promote a full manifestation of the self-incompatibility response.

Brassica↗

PubMed2000

Overgrowth and translocation of Escherichia coli from intestine during prolonged enteral feeding in rats.

Our objective was to examine changes in bacterial flora in the gastrointestinal tract that might lead to their translocation during the prolonged enteral administration of an elemental liquid diet. Eleven rats (experimental group) received a feeding gastrostomy and were administered an intragastric infusion of a liquid elemental diet for 17 days, while 9 rats (control group) received chow and water ad libitum for 17 days. The animals were then killed and the mesenteric lymph nodes (MLNs), liver, jejunum, ileum, and cecum were aseptically removed, homogenized, and cultured for isolation of bacteria. A statistically significant increase in the population of a single species of Enterobacteriaceae, Escherichia coli, was observed in the ileum and cecum of the experimental group fed the liquid diet as compared with the control group. Five of the MLNs from the 11 experimental rats (45%) were positive for E. coli, as compared with none from the 9 control rats, a statistically significant difference (P = 0.038). The E. coli isolated from the MLNs and the ileum of the experimental group exhibited the O168:H serotype. Our results suggest that E. coli isolated from the MLNs of the experimental group had translocated from the intestine. The animal model described here may be useful in screening for drugs to reduce the problem of translocation of intestinal bacteria.

Animals↗

PubMed2000

Malignant islet cell tumor projecting into the main pancreatic duct.

We report herein a rare case of islet cell tumor showing a unique growth pattern in a patient who developed repeated acute pancreatitis as the tumor's initial symptom. Preoperative imaging examinations showed dilatation of the main pancreatic duct (MPD) and cysts around the pancreatic tail. A distal pancreatectomy with splenectomy was performed because the pancreatitis was localized in the distal pancreas and was not controlled by various drug therapies. Grossly, the tumor consisted of two component parts: a markedly infiltrative part in the pancreatic parenchyma, and a papillary elevated part in the MPD. The MPD was obstructed by the tumor spreading widely along the distal MPD. Microscopically, the tumor was composed entirely of islet cell tumors (nonfunctioning), with several foci of venous and lymphatic involvement. Based on its growth behavior, we assumed that the tumor may have arisen from the MPD or from islet cells closely adjacent to the MPD. The patient's postoperative course was uneventful and he is doing well 2 years after the operation. We discuss the growth pattern of the tumor and the cause of the pancreatitis.

Acute Disease↗

PubMed2000

Thoracic esophagectomy combined with pylorus-preserving pancreatoduodenectomy in a one-stage procedure: report of a case.

We present herein the case of a patient who underwent a thoracic esophagectomy and pylorus-preserving pancreatoduodenectomy in a one-stage procedure for synchronous double primary cancer of the thoracic esophagus and the head of the pancreas (intraductal papillary tumor). To reconstruct the upper alimentary tract with the stomach, the gastroduodenal artery and right gastroepiploic vein were successfully skeletonized and preserved. The postoperative course was uneventful and the patient is currently well, 5 years after surgery.

Aged↗

PubMed2000

Composite tumor with papillary adenocarcinoma and squamous cell carcinoma of the esophagus: report of a case.

Papillary adenocarcinoma is extremely rare in the squamous epithelium-lined esophagus. The histopathologic and immunohistochemical characteristics were examined in a composite tumor showing distinct papillary adenocarcinoma and squamous cell carcinoma of the esophagus resected from a 66-year-old man. The esophageal tumor consisted both grossly and histologically of two distinct components: an ulcerative part showing a squamous cell carcinoma, and a polypoid part corresponding to a papillary adenocarcinoma. In addition, the in situ squamous cell carcinoma was contiguous with the esophageal tumor. Mucin secretion was found only in the papillary adenocarcinoma component. Immunohistochemically, tumor cells of the papillary adenocarcinoma component were positive for carcinoembryonic antigen, secretory component, and lactoferrin. These staining patterns were similar to those of the normal esophageal gland proper. These histologic, mucin-histochemical, and immunohistochemical findings suggest that the papillary adenocarcinoma originated from the submucosal esophageal gland and the squamous cell carcinoma from the squamous epithelium lining the esophagus.

Adenocarcinoma, Papillary↗

PubMed2000

Nonoperative treatment of reperforated duodenal ulcer: report of three cases.

During the period between 1983 and 1998, a total of 58 patients were admitted to the surgical department of Akita Red Cross Hospital with acute duodenal perforation. Of these 58 patients, 16 were treated operatively and 42 were treated nonoperatively. Among the 38 men and 4 women who received nonoperative treatment, 3 developed reperforation. The incidence of reperforation was 7.1% and the mean average interval from the initial treatment until reperforation was 3.5 years. Endoscopic biopsy and/or serum anti-H. pylori IgG measurement revealed Helicobacter pylori infection in all three patients. No serious complications developed during the nonoperative treatment of reperforation in these three patients, and their recovery was uneventful. The hospital stay ranged from 10 to 18 days, with a mean stay of 12 days after the first perforation and from 14 to 18 days, with a mean stay of 15.6 days after the reperforation. Nonoperative treatment proved successful as a life-saving procedure for reperforation of a duodenal ulcer in all three patients.

Adult↗

PubMed2000

Key factors influencing bowel function after ileal W-pouch anal anastomosis: a spectral analysis of W-pouch motor activity.

Restorative proctocolectomy with ileal pouch anal anastomosis (IPAA) has become the standard surgical procedure for ulcerative colitis (UC). The purpose of this study was to determine which factors are important to achieve good anal continence after IPAA in terms of the motor activity and pressure-volume relationship. A total of 17 patients with UC who underwent IPAA were evaluated. The internal ileal pouch pressure was transanally measured with and without volume-loading of the pouch which induces the urge to evacuate. The maximum tolerable volume (MTV), first urge volume (FUV), and ileal pouch compliance were calculated and the internal ileal pouch pressure records were subjected to spectral analysis for intensive evaluation of the intraluminal pressure waves. The FUV, correlation of the compliance of the FUV with MTV, and the remaining volume up to the MTV (RVMTV) were analyzed. Compliance of the FUV was significantly correlated with the RVMTV (r = 0.736, P < 0.01). The frequency of the phasic waves in the pouch decreased with length of follow up, reflecting improved function (r = -0.588, P < 0.05). The findings of this intensive analysis of manometric measurement indicate that the key factors in postoperative pouch function are RVMTV and the frequency of phasic waves in the W-pouch.

Adult↗

PubMed2000

Late-type recurrence at the port site of unexpected gallbladder carcinoma after a laparoscopic cholecystectomy: report of a case.

A 73-year-old woman had a laparoscopic cholecystectomy for unexpected gallbladder cancer and 9 days later underwent both a liver bed resection and lymph node dissection. Four years later, she underwent a further resection of a port site recurrence of gallbladder cancer and no other site of recurrence was observed. The seeding of cancer cells during the removal of the resected gallbladder might have caused this tumor. This case may show that the port site recurrence did not necessarily indicate an incurable stage of the disease. In addition, an excision of the recurrent tumor also appeared to eliminate the disease in the patient.

Abdominal Muscles↗

PubMed2000

Changes of the sacrum in severe spondylolisthesis: a possible key pathology of the disorder.

Deficient development of the posterior lumbosacral portion has been thought to be a possible etiology of severe spondylolisthesis. However, the precise causes of the deformity have yet to be revealed. To our knowledge, progression of the disorder has not been discussed in the light of sacral changes on magnetic resonance imaging (MRI). The objectives of the present study were to document changes of the sacrum on MRI scans in patients with severe spondylolisthesis and to discuss the relation of these changes to the progression of deformities. Roentgenograms and MRI scans of 13 patients (10 women and 3 men) with severe spondylolisthesis were retrospectively reviewed. Average age at first MRI examination was 20 years (range, 12 to 50 years). The MRI scans commonly showed a defect at the antero-superior portion of the sacrum. This lesion seems to appear during the period of progression of slipping associated with lumbosacral kyphosis. The defect of the sacrum was considered a unique feature to discriminate this type of olisthesis from others. Taking into consideration the present results, the deformity can be called kyphospondylolisthesis.

Adolescent↗

PubMed2000