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Biomedical subjects

K Hashiguchi

Publications and source records attributed to K Hashiguchi.

At least 73 records · Page 4Linked to original sources
PubMed1988

Chlamydia trachomatis: a currently recognized pathogen of tonsillitis.

The authors investigated whether or not Chlamydia trachomatis could be isolated from tonsillar crypts in order to establish directly the relationship of the organism to the tonsillar infection. In 17 of 65 (26.2%) cases with tonsillitis, C. trachomatis was recovered from tonsillar crypts. Ten of the 17 Chlamydia-positive patients were attended for recurrent sore throat and 5 for lingering tonsillitis. Thirteen of the 17 cases had serum antibody to C. trachomatis. We also isolated this microorganism from one of 18 persons complaining of a lumpy throat. Eleven of the 18 Chlamydia-positive patients had pertinent histories of oro-genital sexual activity, and pharyngeal infection apparently resulted from direct inoculation. The available data suggest that tonsillitis, the most common problem in otorhinolaryngology, may be caused by C. trachomatis more often than has been suspected.

Antibodies, Bacterial↗
PubMed1988

A study on the adjuvant activity of palatine tonsil.

The involvement of non-specific factors in the onset of focal infection should never be ignored. We pay attention to the adjuvant activity of tonsil as one of various kinds of nonspecific factors. We confirmed the presence of such substances with adjuvant activity as lipopolysaccharides and muramyl dipeptide in the lacunar debris of tonsil. Then, using experimental animals with adjuvant induced arthritis, the study was made to determine whether the lacunar debris actually exerts adjuvant activity. We demonstrated the presence of adjuvant activity in the lacunar debris.

Adjuvants, Immunologic↗
PubMed1986

Amplification of the amyE-tmrB region on the chromosome in tunicamycin-resistant cells of Bacillus subtilis.

In a class of tunicamycin-resistant mutants (tmrA7) of Bacillus subtilis, the production of extracellular alpha-amylase is increased by about five fold. The tmrA7 characteristics (tunicamycin resistance and hyperproduction of extracellular alpha-amylase) can be transferred to recipient cells by transformation. In the transformants and the original tmrA7 mutant, typical amplification of the region from 4 kb upstream of the amyE gene to the tmrB gene on the chromosome was detected. The repeating unit, 16 kb in size, repeats tandemly about five and ten times in the mutant and transformants, respectively, and the alpha-amylase production is proportional to the copy number of the amyE gene. Simultaneous amplification of the tmrB gene, which is responsible for tunicamycin resistance in the multicopy state, and the alpha-amylase structural gene (amyE) seems to be the cause of the pleiotropy of the tmrA7 mutation.

Bacillus subtilis↗
PubMed1986

Amplification and deletion of the amyE+-tmrB+ gene region in a Bacillus subtilis recombinant-phage genome by the tmrA7 mutation.

A 22.4-kilobase DNA fragment containing the tmrA7-amyR2-amyE+-tmrB+-aroI+ region of the Bacillus subtilis N7 chromosomal DNA was cloned into a recombinant B. subtilis bacteriophage, p11-AA248. The amyE+-tmrB+ gene region, approximately 12.6 kilobases, in the phage genome was amplified in a tunicamycin-resistant (Tmr) Amy+ AroI+ transductant of B. subtilis by p11-AA248. On the other hand, the amyE+-tmrB+ region in the genomes of 80 to 90% of the phage particles was deleted when the phages were induced from the Tmr Amy+ AroI+ transductants by treatment with 1.0 micrograms of mitomycin C per ml. From analyses of the physical maps and DNA nucleotide sequences in the junction region of the deleted phage genome and the parental DNA fragments, it is suggested that the deletion occurred within a direct repeat sequence composed of 18 base pairs. The endpoints of the amplified gene region seemed to be closely related to both terminal regions of the deleted DNA.

Bacillus subtilis↗
PubMed1986

Essential structure in the cloned transforming DNA that induces gene amplification of the Bacillus subtilis amyE-tmrB region.

Bacillus subtilis B7, a mutant which acquired gene amplification of the amyE-tmrB region, showed, as a result, hyperproductivity (about a 5- to 10-fold increase) of alpha-amylase and tunicamycin resistance. The mutational character was transferred to recipient cells by competence transformation. A 14-kilobase (kb) EcoRI chromosomal DNA fragment of strain B7 was found to have the transforming activity. We cloned a 6.4-kb EcoRI fragment on a phage vector lambda Charon 4A through a spontaneous deletion of 7.6 kb from the 14-kb fragment and subcloned a 1.6-kb HindIII fragment on pGR71. The cloned 6.4-kb EcoRI and 1.6-kb HindIII fragments retained the transforming activity of inducing gene amplification of the amyE-tmrB region. At the junction point (J) of the repeating units (16 kb), the tmrB gene was linked to a DNA region (M) located 4 kb upstream of amyE. The essential structure of the cloned, transforming (gene amplification-inducing) DNA was deduced to be that around J. The subcloned 1.6-kb HindIII fragment that retained the transforming activity was shown to be almost solely composed of the tmrB-J-M region. In addition, the DNA sequence around J was determined.

Bacillus subtilis↗
PubMed1983

[Case of simultaneous triple cancers of the duodenal papilla, gallbladder and stomach].

The patient was a 76-year-old man with simultaneous triple cancer arising in the duodenal papilla, gallbladder and stomach, whose complaint was epigastralgia. Endoscopic and radiologic studies revealed early gastric cancer (IIc) at the posterior wall of the antrum; ERCP disclosed obstruction with "apple core" appearance in the distal bile duct. Pancreaticoduodenectomy, cholecystectomy and distal gastrectomy with lymph node dissection were performed. Histopathological examination of the resected specimens revealed papillo-tubular adenocarcinoma in the duodenal papilla, well differetiated tubular adenocarcinoma in the gallbladder, and moderately differentiated tubular adenocarcinoma in the gastric mucosa.

Adenocarcinoma↗
PubMed1981

[A Lance-Adams syndrome of a diver (author's transl)].

A 39 years old man with severe action myoclonus and epileptic seizure was reported. He had been in good health and worked as an occupational diver until August 16, 1966, when he had to dive into the sea bottom about 35 m deep without diving equipment. After finishing his work, he fell into unconsciousness and drowned. Sometime later, he was rescued and treated as decompression sickness. When he became alert, severe action myoclonus and epileptic seizure appeared. 8 years later, he was admitted to our hospital for rehabilitation. The danger of diving without equipment was indicated, because in sea bottom alveolar oxygen pressure (Pao2) is about 450 mmHg and alveolar air pressure 4.5 atmospheric pressure. In this condition patient could hold his breath longer time than in sea surface. When he finished his work and floated with muscle relaxing, however, his blood supply to the brain decreased. It was thought, therefore, that he fell into unconsciousness and drowned. In somatosensory evoked potentials (SEP) by median nerve stimulation, giat patterns were recognized but there were differences between SEP of myoclonus epilepsy and that of this patient. P50 and N70-80 were giant in him, suggesting that he had a brain stem disturbance. Since the inhibition system of fast conducted fiber did not work well in this state, the component of slow conducted fiber was magnified. Action myoclonus may be due to the disturbance of inhibition system in the brain stem.

Adult↗
PubMed1981

An extremely attenuated, live measles virus vaccine (CAM-EX). Persistence of low titer-antibody in institutionalized children with psychomotor retardation after vaccination.

Fifty children with psychomotor retardation, who had no detectable measles hemagglutination-inhibition (HI) antibody, were vaccinated with an extremely attenuated, live measles virus vaccine (CAM-EX). Only 10% of the children had fever after vaccination. No other symptoms were observed. Forty of 44 vaccinated children showed increase in HI antibody titers of 2(3) or more, with a geometric mean titer of 2(4.2). A five-year follow-up study revealed that the initially low-titer antibodies persisted well under conditions without a booster response due to natural measles.

Adolescent↗