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Biomedical subjects

K Haranaka

Publications and source records attributed to K Haranaka.

47 records · Page 3Linked to original sources
PubMed1981

Research on the production site of tumor necrosis factor (TNF).

Tumor Necrosis Factor (TNF) is an active component of serum taken from Corynebacterium parvum (C. parvum) infected mice treated with lipopolysaccharide (LPS). To locate the production site of TNF, we tried to block TNF production by using the following reagents; carrageenan, hydrocortisone, and trypan blue. Following the injection of a large dose of carrageenan, administered before C. parvum treatment, TNF production was completely blocked. However, when administered after C. parvum treatment but prior to LPS injection, no blockage was observed. Injecting hydrocortisone before the LPS injection also blocked TNF release. However, this treatment, when administered before the injection of C. parvum, had no observable influence on TNF production. A large dose of trypan blue, administered before the LPS injection, also blocked the release of TNF. A low dose of trypan blue resulted in only partial blockage of TNF production. A large dose of trypan blue, administered prior to C. parvum treatment, also showed partial blockage of TNF production. Macrophage-enriched peritoneal exudate cells (PEC), taken from mice infected with C. parvum, released TNF into the supernatant after stimulation with LPS. These results strongly suggest that the production site of TNF is located within the activated macrophage and deeply related with lysosome.

Animals↗
PubMed1980

Endotoxin-induced tumor necrosis factor.

The serum of BCG-infected mice treated with endotoxin contains a substance (tumor necrosis factor, TNF) which mimics the tumor-necrotizing action of endotoxin itself. TNF is not residual endotoxin, but a factor released from host cells, probably macrophages. TNF induced in the same way in rats and rabbits also causes necrosis of transplanted murine tumors. Unlike endotoxin, TNF is toxic in vitro for neoplastic murine and human cell lines but not for mouse embryo culture. TNF has striking effects on immunologic reactions in vitro, some like those of endotoxin and others unlike those of endotoxin. TNF is a glycoprotein; its molecular weight is less than 70,000. Highly purified preparations do not contain lysosomal or nonlysosomal serum enzymes, interferon or prostaglandin E1.

Animals↗
PubMed1977

The enhancement of phagocytosis and intra-cellular killing of Pseudomonas aeruginosa and its common antigen (OEP) coated latex particles by mouse spleen macrophages to which anti-OEP-IgG and gentamicin have been added.

This experiment was performed using macrophages derived from mouse spleens immunized or non-immunized with the common antigen (OEP) of Pseudomonas aeruginosa. The phagocytosis of these macrophages of live or formalin killed Pseudomonas aeruginosa and latex particles coated with OEP were enhanced by the addition of anti-OEP-IgG, but not by immunoglobulin taken from normal mice. The macrophages from mice immunized with OEP showed significantly enhanced phagocytosis of latex particles coated with OEP, but not of live or killed bacteria. The effects of anti-OEP-IgG and gentamicin on phagocytosis and intracellular killing were compared in cases where only anti-OEP-IgG or only getamicin were used as well as in cases where the two were combined. It was found that the two combined was far more effective on phagocytosis and intracellular killing. Furthermore, there was little or no difference in the results depending on whether macrophages from mice immunized or non-immunized with OEP were used.

Animals↗
PubMed1975

Combination therapy of anti-endotoxin antibody and gentamicin in the immunosuppressed mice with Pseudomonas aeruginosa infection.

Therapeutic effect of anti-endotoxin antibody in combination with or without an antibiotic, gentamicin, was studied in DD-strain mice experimentally infected with Pseudomonas aeruginosa. Mice were treated by various immunosuppressive agents such as 60Co irradiation, cyclophosphamide, azathioprine or cortisone acetate prior to infection. Anti-endotoxin antibody was made in mice by immunization with original endotoxin protein (OEP). F(ab')2 fragment of IgG prepared from pooled immune sera was administered intravenously without side effects. Large dose of the antibody resulted in remarkable therapeutic effect even in absence of the antibiotic. Small dose of the antibody enhanced the therapeutic effect of gentamicin. Differential effect of the immunosuppressants for T and B lymphocyte populations was examined by anti-theta cytotoxicity test. Resistance of DD-strain mice to P. aeruginosa infection was not related to these lymphocyte population changes.

Animals↗