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Biomedical subjects

K Haraguchi

Publications and source records attributed to K Haraguchi.

At least 127 records · Page 7Linked to original sources
PubMed1991

Two immunologically different isozymes of ascorbate peroxidase from spinach leaves.

Two isozymes of ascorbate peroxidase (AP) from spinach leaves were separated by hydrophobic chromatography and designated AP-I and AP-II. They had similar molecular weights of about 31,000, as determined by gel-filtration, and showed high specificity for ascorbate. One of the two isozymes (AP-II) was purified to homogeneity by SDS-PAGE. Immunoblotting confirmed that antiserum against AP-II reacted with AP-II but not with AP-I. This antiserum inhibited the activity of AP-II, but not that of AP-I. The amino acid composition and partial amino acid sequence of AP-II were determined.

Amino Acid Sequence↗
PubMed1991

Isolation and characterization of recombinant human prorenin in Chinese hamster ovary cells.

Recombinant human prorenin (rh-prorenin) was purified from supernatants of Chinese hamster ovary (CHO) cell line transfected with the cDNA for rh-prorenin by employing a simple two-step procedure which consisted of ammonium sulfate precipitation and immunoaffinity chromatography using a monoclonal antibody specific for the profragment of human prorenin. About 100-fold purification with 35% recovery was achieved after the two steps. Purified rh-prorenin migrated as a single protein band with apparent molecular weights of 46,000-47,000 and about 50,000 on SDS-PAGE and gel filtration (HPLC), respectively, although it consisted of multiple components (pI values, 5.6-6.4) that could be resolved by isoelectric focusing (IEF). The treatment of rh-prorenin with endo-beta-N-acetylglucosaminidase converted the rather broad protein band to a sharp band on SDS-PAGE and reduced the number of multiple pI peaks on IEF. Amino-terminal sequence analysis of both the purified rh-prorenin and rh-renin revealed Leu-Pro-Thr-Asp- and Leu-Thr-Leu-Gly-, respectively, which agreed with those predicted from the base sequences of their cDNA. These data suggested that microheterogeneity of rh-prorenin is due to the carbohydrate moiety, but not to the protein moiety. Purified rh-prorenin was almost inactive, but was cleaved at the carboxyl end of a dibasic pair Lys-2-Arg-1 by trypsin and converted to active renin. However, at the early stage during trypsin activation, new intermediate forms between rh-prorenin and rh-renin were formed, suggesting multiple activation steps of rh-prorenin in addition to the one step activation.

Amino Acid Sequence↗
PubMed1991

[Retropharyngeal hematoma following a bruise of head and neck--a case report and review of literature].

Diseases affecting the retropharyngeal space are relatively uncommon, much less a case of retropharyngeal hematoma. However, a space-occupying lesion in this area can be life threatening and require emergency surgical intervention. There have been 46 cases of retropharyngeal hematoma reported in the English literatures from 1934 to 1989, but there has been no reported case in Japan. Recently we treated a 72-year-old male sustained retropharyngeal hematoma following a bruise of head and neck. The patients was received tracheostomy, antibiotic therapy and intravenous hyperalimentation, and this hematoma was resolved in about 2 weeks. The etiologies, diagnosis, therapies in our case and prognoses of the literature reviewed 47 cases of retropharyngeal hematoma were discussed comprehensively.

Aged↗
PubMed1991

Effects of 3-methylsulphonyl-4,5,3',4'-tetrachlorobiphenyl and 7,8-benzoflavone on aryl hydrocarbon hydroxylase activity in Ah responsive and Ah nonresponsive strains of mice.

In general, C57BL/6NQdj (C57) and DBA/2JCrj (DBA) strains of mice are considered to be the aryl hydrocarbon (Ah) responsive and Ah nonresponsive strains of mice, respectively, which are determined by whether the hepatic aryl hydrocarbon hydroxylase (AHH) activity is enhanced (Ah responsive) or not (Ah nonresponsive) after the treatment of 3-methylcholanthrene (MC). In this study, first, we examined that the Ah responsiveness was systemically regulated in the lungs and kidneys as well as in the liver and observed its systemic control in these three organs in the two strains of mice. Then, we prepared the hepatic microsomes of the two strains of mice after the treatment of MC (42 mg/kg, once), 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD, 20 micrograms/kg, 6 times) and 2,3,4,7,8-pentachlorodibenzofuran (PenCDF 60 micrograms/kg, 6 times) in order to investigate the effects of 3-methylsulphonyl-4,5,3',4'-tetrachlorobiphenyl (3-MSF-TCB, 1.5-45 micrograms/ml) and 7,8-benzoflavone (ANF, 1.4-42 micrograms/ml) on the respective hepatic microsomal AHH activities and the following results were obtained. 1. As compared with the control enzyme activity, TCDD-induced AHH activity was the highest, PenCDF-induced one the second and MC-induced the lowest in both strains of mice. The inductions of the enzyme activity by these chemicals were much more remarkable in the Ah responsive C57 strain than those in the Ah nonresponsive DBA strain. 2. 3-MSF-TCB and ANF enhanced or reduced the enzyme activity depending on both their concentrations and kinds of microsomes, namely, those prepared from untreated control mice and mice treated with MC, TCDD or PenCDF.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗
PubMed1991

[Elimination of PCDF and PCB congeners in the blood of patients with PCB poisoning in Taiwan].

The blood of 3 Yu-cheng patients sampled from 1980 to 1989 were analyzed for congeners of polychlorinated dibenzofurans (PCDF) and polychlorinated biphenyls (PCB) by high resolution gas chromatography-mass spectrometry and gas chromatography-electron capture detection. The high concentrations of 2,3,4,7,8-penta-, 1,2,3,4,7,8-hexa- and 1,2,3,4,6,7,8-hepta-CDFs (15.4, 37.7 and 4.8 ppb in lipid basis, respectively) were eliminated from the blood at half-lives of 2.14, 2.56 and 2.32 years, respectively. The high concentrations of 8 PCB congeners in the blood were also decreased with time at half-lives of 1.16-4.56 years. The half-lives of retainable PCB congeners were about 2 times longer than those of the PCDF congeners.

Adult↗
PubMed1991

[PCB methyl sulphone: comparison of tissue levels in Baltic grey seals and a Yusho patient].

Methyl sulphone metabolites of PCB and DDE were isolated from different tissues of a Baltic grey seal (Halichoerus grypus). Main components in the seal blubber were identified as 3-MeSO2-2,2',4',5,5'-pentaCB, 4-MeSO2-2,2',4',5,5'-pentaCB, 3-MeSO2-p,p'-DDE, 4-MeSO2-2,2',3',4',5-pentaCB and 4-MeSO2-2,2',3,4',5',6-hexaCB. Liver and lung in the seal contained different MeSO2-PCB pattern compared to all other tissues. These levels in the both tissues were estimated to be 28 and 15 ppm (lipid basis) which corresponded to the same level as the PCB. Concentrations of MeSO2-PCB in any tissues of a Yusho patient were low compared to those in the seal.

Animals↗
PubMed1990

Aryl hydrocarbon hydroxylase activity levels in the hepatic microsomal fraction from MC- or TCDD-treated mice: a comparison between aromatic hydrocarbon responsive and non-responsive strains.

The effects of in vivo administration of polycyclic aromatic hydrocarbons on the levels of aryl hydrocarbon hydroxylase (AHH) activity in aromatic hydrocarbon (Ah) responsive and non-responsive strains of mice were studied using the hepatic microsomal fraction. Injection of 3-methylcholanthrene (MC; 42 mg/kg body wt.) or 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD; 120 micrograms/kg body wt.) into both strains produced marked enhancement of AHH activity except for MC treatment of Ah non-responsive strains. Addition of 7,8-benzoflavone (BNF) to the microsomal AHH assay mixture prepared from mice previously injected with vehicle (olive oil) alone caused an increase in activity when the mice were responsive, while BNF lowered the activity in non-responsive strains. With regard to MC-injected mice, BNF and 3-methyl-sulphonyl-4,5,3',4'-tetrachlorobiphenyl (3-MSF-TCB) decreased microsomal AHH activity in Ah-responsive mice, whereas these drugs enhanced the activity in Ah-non-responsive strains. 3-MSF-TCB also had inhibitory potency on AHH activity, but the mechanism of inhibition seems to be somewhat different from that of BNF. It may also suggest that cytochrome P-450 isozymes inhibited by BNF are different from those inhibited by 3-MSF-TCB.

Animals↗
PubMed1990

Isoproterenol stimulates shift of G proteins from plasma membrane to pinocytotic vesicles in rat adipocytes: a possible means of signal dissemination.

Guanine nucleotide-binding regulatory proteins (G proteins) are linked to a large number of surface membrane receptors and appear to regulate a variety of effector systems located both in the plasma membrane and in other parts of the cell. The mechanism of the disseminative actions of G proteins remains obscure. During an investigation of the fate of two types of G proteins, Gs and Gi, in rat adipocytes, we unexpectedly found that isoproterenol, which stimulates cAMP levels and lipolysis in these cells, induces parallel increases in both Gs and Gi in a low-density microsomal fraction rich in endosomes and Golgi bodies. Two plasma membrane constitutive enzymes, adenylyl cyclase and 5'-nucleotidase, are also elevated in this fraction. NaF and NaN3, metabolic inhibitors, block the redistribution process. The isoproterenol-stimulated shifts are completely reversible after removal of the hormone, indicating a recycling, endocytic process. The endocytic process seems to be fluid phase endocytosis, or pinocytosis, since isoproterenol stimulates the uptake of both fluorescent-labeled dextran and horseradish peroxidase into the same vesicles containing Gs. However, the vesicles that accumulate in response to isoproterenol seem heterogenous in properties that may reflect the lipolytic process induced by isoproterenol. It is speculated that the "pinosomes" formed in response to lipolytic hormones may continually produce signals within the cellular interior during their processing and cycling. Hence, signal production in response to hormones need not be confined to the cell membrane; circulating pinosomes may be responsible for some of the disseminative effects of hormones.

Adenosine Diphosphate Ribose↗
PubMed1990

In vitro effects of methylsulfonyl polychlorinated biphenyls and 7,8-benzoflavone on aryl hydrocarbon hydroxylase activity in human lymphoblastoid cells.

The effects of 11 isomers of methylsulfonyl polychlorinated biphenyls (MSF-PCBs) on the aryl hydrocarbon hydroxylase (AHH) activity were examined at a fixed dose of 1.5 micrograms/ml by using cultured lymphoblastoid cells. One of the isomers 3-MSF-3',4,4',5-tetraCB, as well as 4-MSF-3,3',4',5-tetraCB and 4-MSF-3,3',4',5,5'-pentaCB, effectively reduced the AHH activity when added to the culture medium, especially that previously treated with 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) to induce the AHH activity. However, the inhibitory potencies exerted by the above three MSF-PCB compounds were smaller than that by 7,8-benzoflavone (BNF). When added to the culture medium simultaneously with TCDD or 3-methylcholanthrene (MC), 3-MSF-3',4,4',5-tetraCB blocked the enhancement of the AHH activity by TCDD but did not that by MC. This is in contrast to BNF which blocked the increases due to both TCDD and MC. On the other hand, 3-MSF-3',4,4',5-tetraCB added directly to the reaction mixture for the TCDD-induced AHH activity showed little influence, while BNF decreased the same activity. These results imply that the effect of 3-MSF-3',4,4',5-tetraCB on the AHH activity is different from that of BNF.

Aryl Hydrocarbon Hydroxylases↗
PubMed1990

[Sensorineural hearing loss following the decompression of the facial nerve].

This study aims to ascertain whether sensorineural hearing loss occurs after the decompression of the facial nerve. Nine patients have undergone decompression of the facial nerve (limited to the vertical segment) by the transmastoid approach were selected as the subjects of this study because the influence of disinfectants and surgical trauma on the inner ear seems to be less severe in this operation than in tympanoplasty. Additionally, this approach seems to be more suitable for judgement of the effect of the drilling noise induced acoustic trauma on the inner ear than tympanoplasty. Though high tone abrupt sensorineural hearing loss was occurred in the operated ear in seven patients, no changes in hearing level were found in the contralateral side ear. Bekesy audiometry performed in only one case revealed Jerger II which suggested some disorder in the cochlea. Not only acoustic trauma induced by the drilling noise but also labyrinthitis occurred via the opened facial canal and the internal ear canal seemed to be the cause of sensorineural hearing loss following the decompression of the facial nerve.

Adult↗
PubMed1990

[Statistical studies of student clinical practice. 1. Clinical practice of operative dentistry from 1983 to 1989].

Clinical practice has been assigned as a final and very important aspect of study and practice of dental education. In our university, the students are exposed the clinical practice from the latter term of fifth year. This report investigated the protocols of clinical cases from the first to the seventh class of graduates. The results obtained were as follows: 1) The graduates of first class averaged 9 clinical cases. This decreased with the years and the fourth class averaged 5.5 cases. From the sixth class simulator training and mutual practice among the students was introduced and the total average of seventh class was 5.1 cases. 2) The dental practice centers on restoration and 20 percent of all cases were amalgam fillings from the first to four the class. However this decreased from the fifth class and does not take place at present. Composite resin restorations increased. Cast restorations occupied 20 percent of all cases and the proportion hasn't changed. 3) Clinical cases of graduates of fifth class who were first to include treatment between students averaged 7 cases. Mutual treatment practice is useful because of the increase exposure to clinical practice.

Clinical Clerkship↗
PubMed1990

[A study for non-asbestos casting liners. An experimental study of the adaptability of MOD casting to die].

Asbestos liners for investment metal casting has frequently been used in dental laboratory work. In recent years, the dangerous properties of asbestos to the human body were reported in Europe and the United States, and casting liners without asbestos were developed and marketed by many manufacturers. These are so-called non-asbestos liners. This study evaluated the adaptability of MOD inlay castings to dies using 8 kinds of commercial non-asbestos liners of ADA standard No. 2 specifications. The results were as follows; Single and double layers of Casting ribbon, New Asbestos Ribon, Shofu experimental, and KAOLIN showed good adaptation. Shofu experimental with a single layer showed the best marginal adaptation (10 microns). OVAL LINERS and CASTING LINERS with single layer showed good adaptation, but with double layers the adaptation was poor. In FLASK LINER, the double layer showed better adaptation than that with a single layer. Most non-asbestos liners used in this study are adequate in useful to MOD inlay castings.

Asbestos↗
PubMed1989

[Experimental study on the air streams in the paranasal sinus].

Air streams in the paranasal sinus were investigated using Nose-Sinus Model to contribute to the fundamental knowledge of aerosol therapy. The results obtained were as follows; 1. The velocity of air streams in the paranasal sinus was measured by Laser Doppler Anemometer and the visualization of streams was done by Laser Light Sheet method. 2. The amount of aerosol passed into the maxillary sinus is approximately in proportion to the sectional area of the ostium. 3. A considerable amount of aerosol passing into the sinus was noted under LLS technique applying positive pressure from the choana. From the above results, it is necessary for the effective entrance of aerosol into the sinus first to open the middle meatus wide and to add the intermittent pressure.

Aerosols↗
PubMed1989

[Identification of polychlorinated phenyldibenzofurans (PCPDFs) in the casual rice oil associated with yusho].

The polychlorinated dibenzofuran (PCDF) fractions obtained from the causal rice oils associated with yusho were analyzed by capillary column gas chromatography with electron capture detection (ECD/GC) and capillary column gas chromatography-mass spectrometry (GC/MS) in negative chemical ionization (NCI) mode. The GC/MS analysis revealed that the PCDF fraction contained, in addition to PCDF congeners, penta-to octa-and probably nona-chlorinated phenyldibenzofuran (PCPDF) congeners in comparison with retention times and NCI mass spectra of PCPDF standards. The ECD/GC analysis showed the presence of at least 30 different PCPDF congeners in the rice oils.

Food Contamination↗
PubMed1989

[Effects of 3-methylsulphonyl-4,5,3',4-tetrachlorobiphenyl and 7,8-benzoflavone on aryl hydrocarbon hydroxylase activity].

First, we examined the effects of 3-methylsulphonyl-4,5,3',4',-tetrachlorobiphenyl (3-MSF-TCB, 1.5 ppm) and 7,8-benzoflavone (ANF, 1.4 ppm) on aryl hydrocarbon hydroxylase (AHH) activity in cultured human lymphoblastoid cells and we have obtained the following results: (1) 3-MSF-TCB preferentially inhibited the enzyme activity induced by 2,3,4,7,8-pentachlorodibenzofuran (PenCDF) or 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and slightly stimulated the enzyme activity induced by 3-methylcholanthrene (MC). (2) ANF reduced well the AHH activities induced by all the three enzyme inducers and more strongly inhibited the PenCDF- or TCDD-induced enzyme activity (about 89%). (3) About 64 and 76% of basal (uninduced) AHH activity was lowered by 3-MSF-TCB and ANF, respectively. Second, we investigated the effect of 3-MSF-TCB (1.5-45 ppm) and ANF (1.4-42 ppm) on AHH activity of hepatic microsomes from the Ah nonresponsive (DDD) and responsive (C3H) strains of mice and we have got the following results: (1) 3-MSF-TCB and ANF enhanced or reduced the enzyme activity depending on both their concentrations and kinds of microsomes, namely, those prepared from untreated mice (control-microsomes) and mice treated with MC (MC-microsomes) or TCDD (TCDD-microsomes). (2) ANF showed higher potency for both the enhancement and the inhibition of the enzyme activity than 3-MSF-TCB. (3) The AHH activity of TCDD-microsomes seemed to be more greatly reduced with either 3-MSF-TCB or ANF than that of MC-microsomes. (4) The effects of 3-MSF-TCB and ANF on the enzyme activity of MC-microsomes of DDD mice and of control-microsomes of C3H mice.

Animals↗
PubMed1989

[Concentration changes in PCB isomers in the blood in patients with PCB poisoning].

The blood of 3 yu-cheng patients sampled from 1980 to 1985 and of 14 yusho patients sampled from 1982 to 1988 were analyzed for 7 polychlorinated biphenyl (PCB) isomers by gas chromatography with a capillary column. The concentrations of PCBs in yu-cheng patients were up to 400 ppb and much higher than those of yusho patients. The high concentration of PCBs were decreased at biological half lives of about 50 months and 2,4,3',4'-tetra-CB and 2,4,5,3',4'-penta-CB were first decreased with time at half lives of about 12 months. Therefore, peculiar gas chromatographic pattern for yusho patients would be formed 3 years after the onset. The concentration and patterns of blood PCBs of yusho patient kept in similar levels during 1982 to 1988.

Adolescent↗