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Biomedical subjects

K Harada

Publications and source records attributed to K Harada.

At least 1,171 records · Page 65Linked to original sources
PubMed1983

Nephropathy induced in rats by repeated intravenous injections of rabbit anti-FX1A antibody.

Repeated intravenous injections of small amounts of rabbit anti-rat FXIA antibodies (RAFA) induced a renal damage with the morphological features of epimembranous glomerulonephritis with significant and persistent proteinuria. These findings indicated that repeatedly injected RAFA accumulated in glomeruli and that there seems to be a threshold in quantity of RAFA localized in glomeruli to induce renal damage with proteinuria.

Animals↗
PubMed1982

Conjugation of drug-resistance plasmids from vibrio anguillarum to Vibrio parahaemolyticus.

Drug-resistant strains of Vibrio anguillarum, a fish pathogen, were isolated from diseased fish in culture ponds. In investigations of these strains, the transfer of resistance to chloramphenicol, tetracycline, and sulfanilamide from multiple resistant organisms to laboratory recipients was observed. The plasmid from V. anguillarum was stably maintained in both recipient strains of Vibrio parahaemolyticus and Escherichia coli. The plasmids isolated from Vibrio anguillarum belong to incompatibility group C. The molecular weight of these plasmids determined by electron microscopic observation was about 103 to 113 megadaltons.

Chloramphenicol↗
PubMed1982

Antigenic association between kidney and thymocyte.

Commonly shared antigens between the renal tissue and lymphocytes, especially the thymocyte were studied in rats by immunofluorescent technique. To clarify the specificity of antisera, absorption analysis was carried out. The brain-associated thymocyte antigen, presumably Thy 1 antigen, was detected by anti-rat thymocyte serum (ATS) or anti-rat brain serum (ABS) not only on the surface of thymocytes but also in renal glomeruli. The distribution or localization of the antigen in glomeruli was considerably different between acetone-fixed and unfixed kidney sections. On the fixed section the antigen was shown exclusively in the glomerular mesangium. However, it appeared to be diffusely dispersed on glomerular tissue on the unfixed section. On the other hand, antisera against kidney-related antigens, anti-Fx1A, anti-GBM, and anti-CIG, did not react with the surface of thymocytes, or they were not absorbed with brain homogenate.

Acetone↗
PubMed1982

Mechanism of inducing autologous antibody formation in nephrotoxic serum nephritis.

The role of the heterologous antibody fixed in the kidney was evaluated by means of transplantation of a nephritic kidney to a normal recipient in the rat. An intravenous injection of 0.5 ml of nephrotoxic serum could not induce distinct changes in the heterologous phase, but it could cause mild or moderate changes in the autologous phase. Titration of rat antibody to rabbit IgG according to passive hemagglutination test showed that rabbit IgG fixed in the transplant could induce autologous antibody formation with a similar intensity to nephrotoxic serum given intravenously, indicating that fixed antigen played the most important role in autologous antibody formation and other factors, such as the kidney-fixing rabbit IgG remained in circulation or fixed in other tissues and the gamma-globulin other than anti-kidney antibody, might be less important. It was unclear which antibody, fixed or dissociated from the transplant, played the more important role in stimulating antibody formation.

Animals↗
PubMed1982

Liberation of serotonin from rabbit blood platelets by bacterial cell walls and related compounds.

A study was made on the activity of various bacterial cell walls and peptidoglycans to liberate serotonin from rabbit blood platelets. All of the test cell walls or peptidoglycans prepared from 27 strains of 21 bacterial species were shown to cause a marked release of serotonin, regardless of differences in types of peptidoglycan and non-peptidoglycan moieties and in some biological properties. The assay made with the water-soluble "digests" of Staphylococcus epidermidis cell wall peptidoglycans, which were prepared by use of appropriate enzymes, revealed that a polymer of peptidoglycan subunits (a disaccharide-stempeptide) was definitely active in the release of serotonin, but a structural unit monomer was inactive. Among a variety of synthetic muramylpeptides and their 6-O-acyl derivatives, only 6-O-(3-hydroxy-2-docosylhexacosanoyl)-N-acetylmuramyl-L-alanyl-D-isoglutaminyl- L-lysyl-D-alanine was found to hold a strong serotonin-liberating activity.

Acetylmuramyl-Alanyl-Isoglutamine↗
PubMed1982

Serum HDL-cholesterol and lipoprotein fraction in Kawasaki disease (acute mucocutaneous lymph node syndrome).

Serum HDL-cholesterol level and lipoprotein fraction were studied in 50 patients with Kawasaki disease (acute mucocutaneous lymph node syndrome), ranging in age from 4 months to 7 years, with an average of 2 years and 6 months. There were 31 males and 19 females. In 7 of these patients coronary arterial aneurysms were revealed by aortogram. In all 50 patients analysis were carried out periodically from the onset of the disease to the convalescent stage, and the following results were obtained: 1) Serum total cholesterol level showed mild change throughout the course of the disease. 2) Average HDL-cholesterol level was 29.6 +/- 11.0 mg/dl in the first week of the disease, 26.8 +/- 8.9 from 1 to 2 weeks and 47.9 +/- 12.6 after 6 weeks. There was significant difference between the HDL-cholesterol levels of first 2 weeks and after 4 weeks (p less than 0.01). 3) The recovery of HDL-cholesterol level was slow in both clinically severe patients and the patients with coronary aneurysms as compared with that in clinically mild patients (p less than 0.05). These findings suggest that there may be some relationships between the alteration of serum lipoprotein and the severity of systemic vasculitis in Kawasaki disease, especially which may play some role in clinical severity and the occurrence of coronary arterial lesions. However, further study is needed to clarify the cause of changes in serum HDL-cholesterol and lipoprotein.

Child↗
PubMed1982

Primary chylopericardium recovered without surgical treatment.--Report of a case and review of the literature.

A case of 7-year-old girl who had recurrent chylopericardium is presented. She was asymptomatic and physical examination disclosed only enlarged cardiac dullness on percussion and distant cardiac sound on auscultation. In despite of numerous pericardiocentesis, institution of medium-chain triglyceride diet and corticosteroid therapy, chylous pericardial effusion persisted. Four years later the amount of pericardial effusion began to decrease and 5 years later it disappeared completely. Her cardiac size became normal on the chest X-ray. She remained totally asymptomatic throughout the course of this disease. If the patient is asymptomatic and can well tolerate daily life, surgery is not necessarily indicated, and the patients should be treated medically as long as possible. A review of previously reported cases are given.

Adolescent↗
PubMed1982

The weighted geometric center in the remote afterloading intracavitary therapy for carcinoma of the uterine cervix.

In this paper, we investigate the weighted geometric center as one of the parameters to reflect the weight of source arrangement of the intracavitary technique for carcinoma of the uterine cervix. Since the nonrigid applicator is used at our department, there are some differences between point-A doses on both sides, and also between the actual and planned doses of point A, in spite of using our pretreatment computerized dose calculation system. Based on this study, it is suggested that the weighted geometric center is reflected in the inclination of the dose distribution and the ratio of actual to planned mgh which means the weight factor in our pretreatment computerized calculation system. It seems likely that the weighted geometric center is one of the parameters to compare the intracavitary technique for carcinoma of the uterine cervix.

Brachytherapy↗