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Biomedical subjects

K Hansen

Publications and source records attributed to K Hansen.

At least 271 records · Page 15Linked to original sources
PubMed1977

Method for comparing films and processing procedures in photofluorography.

A method for comparing measurements of films and processing procedures is described. A stepped wedge is developed on basis of density measurements in defined areas of chest photofluorographic films. Quality criteria for films and processing procedures are established by systemization of film evaluation. Minor modifications of the method are necessary for application elsewhere in diagnostic radiology.

Fluoroscopy↗
PubMed1975

Diffuse vaginal adenosis. Three cases combined with imperforate hymen and haematocolpos.

Diffuse vaginal adenosis is a rare disease presenting severe symptoms and difficult to treat. It has not previously been described in the Scandinavian literature. Three typical cases are reported, two in children of 14 years and one in a women of 20 years, who were all treated for imperforate hymen and haematocolpos. None of the patients had been subjected to oestrogen treatment in utero. The colposcopic findings are very characteristic and are described in relation to the histological examination. The pathogenesis, treatment and risk of malignant transformation are discussed.

Adolescent↗
PubMedDate not supplied

Application of two-dimensional gel analysis to identification and characterization of tyrosine phosphorylated substrates for growth factor receptors.

The technique of two-dimensional gel electrophoresis was used for analysis of tyrosine phosphorylated polypeptide substrates after epidermal growth factor (EGF)-induced stimulation of receptor tyrosine kinase activity in a brush border fraction of human placental syncytiotrofoblast cells. After incubation with [gamma 32P]ATP, followed by autoradiography of the gels, 35 phosphorylated components were detected, of which 8 were strongly tyrosine phosphorylated by EGF. Using a more sensitive assay with phosphotyrosine-specific antibody, an additional 12 polypeptide components were found to be strongly tyrosine phosphorylated by EGF. A number of the phosphorylated substrates could be aligned with components in a protein catalog of the human brush border membrane fraction that was characterized by glycoprotein staining, Triton X-114 fractionation, immunoreaction with specific antibodies, and comigration with 35S-labeled AMA (transformed human amnion) cells. Identified components, stimulated by EGF, in addition to well-recognized substrates (calpactin II, ezrin, EGF receptor) included beta-tubulin and serum albumin, while other cytoskeletal proteins and alkaline phosphatase were excluded as substrates. A notable feature of the catalog was that a number of glycoproteins were present in both the membrane and cytoskeletal fraction, suggesting involvement in membrane/cytoskeletal interactions. The data demonstrate the feasibility of using two-dimensional gel electrophoresis in a global way to identify target substrates for tyrosine kinase activity. In addition they suggest that many of these are located in the vicinity of tyrosine kinase at the membrane/cytoskeletal border at a location which is probably involved, at the molecular level, in morphological changes of the plasma membrane associated with cell proliferation.

Adenosine Triphosphate↗