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Biomedical subjects

K H Milby

Publications and source records attributed to K H Milby.

4 recordsLinked to original sources
PubMed1985

Direct measurement of antigens in serum by time-resolved fluoroimmunoassay.

A long-lived fluorescence label (Tb3+) has been attached to the antigen of interest by using a bifunctional chelating agent 1-(p-benzenediazonium)-EDTA. A nonequilibrium competitive-binding immunoassay protocol, in conjunction with time-resolved detection of the long-lived fluorescence label, allows the antigen to be analyzed directly in samples containing diluted human serum. Results obtained for immunoglobulin G with this simple and rapid procedure correlated well (r = 0.93) with those by a commercially available fluorescence immunoassay method.

Antigens↗
PubMed1981

In vitro and in vivo depolarization coupled efflux of ascorbic acid in rat brain preparations.

The depolarization-coupled efflux of endogenous ascorbate is demonstrated using rat synaptosome preparations and a rat cortical cup method. Ascorbate and catecholamines (monitored as a methodological control) were quantitated by high pressure liquid chromatography with electrochemical detection. In vitro potassium ion induces ascorbate efflux in a concentration-dependent, although calcium ion-dependent, manner. Veratridine also induces ascorbate efflux and its effect can be blocked by tetrodotoxin. In vivo, ascorbate efflux was likewise stimulated by increased potassium ion and by veratridine. In addition, electrical stimulation of medial lemniscus was accompanied by an increased efflux of ascorbate from somatosensory cortex. These results are intriguing in light of the recent evidence for the interaction of ascorbate with several neurotransmitter systems.

Animals↗