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Biomedical subjects

K H Marquart

Publications and source records attributed to K H Marquart.

At least 37 records · Page 2Linked to original sources
PubMed1984

An unusual form of endoplasmic reticulum in mononuclear cells of a giant cell tumor of bone.

A peculiar configuration of endoplasmic reticulum (ER) was observed by electron microscopy in some mononuclear cells from a malignant giant cell tumor of bone that had been removed from a cervical vertebral body in a 54-year-old female. The unusual ER consisted of looped, smooth tubules, which originated from poorly developed rough ER present in the tumor cells. The tubular structures were approximately 23 nm in diameter and contained paracrystalline material. The curvilinear tubular ER closely resembles the vermiform tubular structures that have been observed mainly in histiocytes from different clinical forms of histiocytosis X.

Bone Neoplasms↗
PubMed1983

Intranuclear vermicellar bodies in human osteosarcoma and ossifying fibroma cells.

Electron microscopic investigation of 16 cases of human bone tumor revealed the presence of intranuclear vermicellar bodies (IVB) in cells of two osteosarcomas and one ossifying fibroma. The spherical nuclear inclusions consisted of electron-dense, interwoven, threadlike structures measuring approximately 20-30 nm in diameter. The nature and significance of the IVB remain obscure at present.

Bone Neoplasms↗
PubMed1983

[Iatrogenic pseudotumor of the skin induced by polyvinyl pyrrolidone].

A 65-year-old woman developed a prominent tumor-like swelling of the skin in the face and shoulder area after having received parenteral applications of a drug containing polyvinylpyrrolidone (PVP) (Depot-Impletol) for many years. Upon first examination the alterations appeared to represent a tumor of the adipose tissue. Subsequently, however, they were shown to result from excessive storage of high-molecular fractions of PVP in the reticulohistiocytic system. Storage of these fractions of PVP, which cannot be eliminated with urine, was also found in the interstitial tissue of adjacent muscles and in local lymph nodes. The histological picture resembles a thesaurismosis. The present report also describes enzyme histochemical and electron-microscopic findings. The electron-microscopic results are similar to those described by other authors in the few ultrastructural reports of PVP storage. The ultrastructural alterations in the capillary endothelium suggest a transendothelial transport mechanism for the incorporated substance.

Aged↗
PubMed1979

Pleomorphic C-type virus particles in bone tissue of strain 101 mice and (C3H X 101)F1 hybrids.

The proximal tibial metaphysis of apparently healthy strain 101 mice, 3-4 weeks old, and (C3H X 101)F1 hybrids, 3-48 weeks old, was studied by electron microscopy. Budding, immature, and mature (C-type virus particles were found within trabecular bone tissue of 3 of 8 strain 101 and 4 of 12 (C3H X 101)F1 mice. The particles were most common in lacunae of aging osteocytes and were only occasionally associated with osteoblasts. Although the morphology of budding and immature particles appeared to be identical with that of typical C-type viruses, most of the mature forms of particles showed atypical structure and size. The electron-dense core was very large and not clearly defined, measuring approximately 70-130 nm in diameter. This diffuse core sometimes completely filled the space within the envelope of the particles. The diameters of the pleomorphic mature C-type particles ranged from approximately 90 to 150 nm. The possible association between the production of pleomorphic C-type virus particles by bone cells and spontaneous osteomagenesis in 101 and (C3H X 101)F1 mice is discussed.

Animals↗
PubMed1979

Establishment and characterization of C-type RNA virus-producing cell lines from radiation-induced murine osteosarcomas.

Eight cell lines were established from murine osteosarcomas induced in vivo with the radionuclides 224Ra and 227Th. They have been compared by light and electron microscopy, by karyology, and by their growth properties. The morphology, the growth pattern, and the ability to induce tumors in mice indicate that five of them are tumor cell lines. Chromosome studies demonstrated that the five cell lines have marker chromosomes. The other cell lines only showed some criteria generally used to score for transformation of fibroblasts and they may be derived from stromal cells. All cell lines release virus particles in the culture fluid which have the typical properties of RNA tumor viruses. They possess C-type morphology, a density of 1.16--1.18 g/cm3, a 60--70 S RNA, a RNA dependent DNA polymerase and they induce syncytia in rat XC cells. The possible significance of these virus particles in radiation osteosarcomagenesis is discussed.

Animals↗
PubMed1978

[Intracristal linear inclusions in mitochondria of human rhabdomyoma cells (author's transl)].

Electron microscopic study of a rhabdomyoma of the soft palate of a 50-year-old woman revealed linear structures within the intracristal spaces of mitochondria. In the center of most cristae, an electron-dense line was found located in parallel arrangement to the limiting cristal membrane. The linear inclusion was separated from the cristal membrane by a narrow space of lower electron-density. Thus, when sectioned longitudinally, the abnormal cristae exhibited a pentalaminar appearance. Additionally, they were characterized by a greater diameter than that of normal cristae and a "rigid" configuration. Higher magnification showed that the intracristal line consisted of a row of periodically arranged, electron-dense dots. Sometimes, these seemed to be connected with the wall of the intracristal space by fine, electron-dense cross-striations. In some mitochondria of rhabdomyoma cells, crystals with a linear substructure were observed in both intra- and extracristal spaces. The crystalline bodies located within cristae had probably been formed by accumulation of linear inclusions. The extracristal bodies seemed to have arisen from fusion of pentalaminar cristae. It is supposed that the intracristal linear inclusions in mitochondria of rhabdomyoma cells represent crystallized proteins of mitochondrial enzymes.

Female↗
PubMed1978

[Rhabdomyoma of the soft palate (author's transl)].

A case of a rhabdomyoma of the soft palate of a 50-year-old woman is reported. The histological characteristics of this rare tumor are demonstrated by light and electron microscopic studies. The findings are compared with the criteria of extracardiac rhabdomyoma cases described in the literature.

Adolescent↗
PubMed1977

So-called annular gap junctions in bone cells of normal mice.

Spherical bodies consisting of a granular matrix and a pentalaminar limiting membrane were found in cells from the proximal tibial metaphysis of normal mice. The structures measured about 280-570 min in diameter and were located mainly in the cytoplasm of osteoblasts and occasionally in preosteoblasts. The granules within the bodies resembled ribosomes. The multi-layered composition of the limiting body membrane was identical with that of intercellular gap junctions.

Animals↗
PubMed1977

[The ultrastructure of the neointima of arterial prostheses made of lyophilized human dura. Long-term results in dogs (author's transl)].

Five arterial prostheses made of lyophilized human dura (LHD) were inserted into dogs and removed after 4, 5 1/2, 13, 43, and 46 months to be studied with the electron microscope. The neointima of all grafts consisted of pseudo-endothelial cells, fibrocytes, and collagen fibrils. Additionally, 43 and 46 months after implantation smooth muscle cells and elastic microfibrils were present. Thus, the composition of the neointima was substantially identical with that of the neointima of synthetic arterial grafts and collagen-fabric ones. The clinical application of arterial prostheses of LHD, however, seems at present too early because of degenerative changes in the walls of those grafts which remained nearly 4 years in the dogs.

Animals↗
PubMed1976

Presence of intracisternal type A particles in normal bone tissue from various mouse strains.

Intracisternal type A particles were detected by electron microscopy in cells of normal trabecular bone tissue from the mouse strains C57BL, NMRI, C3H, 101, and (C3H X 101)F1. The relatively small numbers of particles observed in all five strains were roughly equivalent. About 10% of the bone cell profiles examined in specimens from each animal contained a single particle. The intracisternal type A particles occurred predominantly in osteoblasts and rarely in osteoprogenitor cells and osteocytes. They were not found in osteoclasts.

Animals↗
PubMed1976

[So-called fibrous long-spacing collagen in a murine osteosarcoma (author's transl) ].

Peculiar periodically banded structures were found in the extracellular spaces of a vascular type of a murine osteosarcoma. The structures consisted of fine almost parallely arranged filaments with electron-dense cross-bands of about 35-50 nm thickness. The periodicity of the bands was about 85-100 nm. Similar structures have been found by many authors in various tissues of different species. Commonly, they have been referred to as "fibrous long-spacing collagen".

Animals↗