Search PubMed⌕ Search

Biomedical subjects

K Grossgebauer

Publications and source records attributed to K Grossgebauer.

70 records · Page 4Linked to original sources
PubMed1968

Lysozyme production as an aid for identification of potentially pathogenic strains of staphylococci.

Lysozyme production is a frequent property of potentially pathogenic staphylococci. In the present study, 1,186 strains of human origin, 85 strains of animal origin, and 156 strains of Staphylococcus albus (epidermidis) were tested. Of 1,114 coagulase-positive strains of human and animal origin, 1,098 were lysozyme-positive (98.5%). On the other hand, of 157 coagulase-negative strains which, based on further investigations, belong to the potentially pathogenic staphylococci, all were lysozyme-positive. All of the 156 strains (100%) belonging to the species S. albus (epidermidis) were lysozyme-negative. We conclude that lysozyme production is a better index of potentially pathogenic staphylococci than the measurement of free coagulase, especially in cases of strains of animal origin. It is possible that lysozyme production allows a differentiation between pathogenic and nonpathogenic coagulase-negative staphylococci.

Animal Population Groups↗
PubMed1967

Assessment of virucidal ability of chemical disinfectants.

Increasing interest has been shown in studying and testing the virucidal properties of disinfectants. At the moment there are no well-established standard methods available; quite different procedures have been employed. Therefore, there is no satisfactory way of comparing the results obtained in this field. We present some of these problems and propose standard test methods to overcome these difficulties.

Antiviral Agents↗
PubMedDate not supplied

Cancer: re-evolution of eucaryotic to procaryotic replication units--a result of genetic transposition?

We have consistently tried to present our "procaryote-hypothesis" of oncogenesis in the light of evolution. It is suggested that the replication units are the essential starting points of oncogenesis, and cancer is the result of a short and compact re-evolution from eucaryotic to procaryotic replicons. In our view, the process of re-evolution or reactivation of procaryotic replication units and/or their precursors starts with the activity of a cancerogen and proceeds by genetic rearrangements, possibly by genetic transposition. Consequently, some remarks had to be made on: (a) the evolution of self-replicative species; (b) the evolvement of procaryotes to eucaryotes; and (c) the various mobile repeated genetic elements. Moreover, we have taken into consideration the important fact that most of the mammalian genome is reversibly repressed and "that there is in these modern genes still a memory of ancient sequences" ( Eigen et al., 1981). We can only speculate on the extent of the evolutionary conservation, particularly of certain ancient nucleotide sequences related to autonomous, indefinite self-replication. Nevertheless, no real argument can be made against the simple possibility that a re-evolution of eucaryotic to procaryotic replicons lead to cancer.

Animals↗
PubMedDate not supplied

[New fluorescence method for staining of human spermatozoa].

300 human ejaculates from a andrological group of patients were stained for visualisation of the spermatozoal morphology with the new fluorochrome DAPI (4',6-diamidino-2-phenylindol) and examined by fluorescence microscopy. DAPI has proven to be stable, since the preparations do not fade even after long storage periods. It can be utilized in small amounts and has a special affinity for the spermatozoon nucleus, which it stains light yellow, in contrast to the acrosomal parts which are fluorescent green. The method is surprisingly simple and can be carried out in a few minutes. A further advantage of the DAPI procedure lies in the additional staining of leucocytes and microorganisms, so that inflammatory processes of the male accessory organs can be diagnosed as well.

Fluorescent Dyes↗