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Biomedical subjects

K Goto

Publications and source records attributed to K Goto.

At least 973 records · Page 54Linked to original sources
PubMed1980

Anti-anaphylactic activities of a new benzopyranopyridine derivative Y-12,141 in rats and guinea pigs.

The active anaphylactic bronchoconstriction of rats mediated by IgE-like antibody against DNP-Ascaris was inhibited by intravenous and intratracheal treatment with Y-12,141 in a dose-dependent manner. In both routes, the inhibitory effect of Y-12,141 on this response was more potent than that of disodium cromoglycate (DSCG). The oral administration of Y-12,141 also produced a similar inhibition of the response. The passive anaphylactic bronchoconstriction of guinea pigs mediated by IgG-like antibody against egg albumin was also prevented dose-dependently by treatment with Y-12,141 given intravenously, but not with DSCG. The present results suggest that Y-12,141 may be effective for the treatment of allergic bronchial asthma.

Anaphylaxis↗
PubMed1980

Formation of cell-sized single-layered liposomes in a simple system of phospholipid, ethanol and water.

A mixture of an ethanolic solution of phospholipid and water was sonicated, kept at 50 degrees C for 30 min, and then observed by a phase-contrast microscope. A large number of cell-sized, single-layered liposomes thus obtained were different in shape and thickness from the liposomes prepared by conventional methods. Fixation with La(NO3)3 and KMnO4 stabilized the liposomes. As a result they remained unchanged in the organic solvents used for dehydration and embedding. Ultrathin sections of the liposomes could, therefore, be observed with an electron microscope. The nature of these liposomes was discussed in relation to the structure and function of the cell membrane.

Ethanol↗
PubMed1980

Structural studies on stem bromelain. Cyanogen bromide cleavage and amino acid sequence of carboxyl-terminal half of the molecule.

Stem bromelain was cleaved with cyanogen bromide, and the products were fractionated with and without prior maleylation and sulfitolysis. The fragments that corresponded to the carboxyl-terminal half of the molecule were isolated and nearly completely sequenced. This portion of the enzyme molecule contained one disulfide linkage. A specific cleavage at the amino peptide bonds of that cystine residue by reduction, modification into S-cyano derivatives and exposure to alkali gave important information of the amino terminal sequence. By combining the present data with the previously known partial sequence of the parent molecule, 101 amino acid residues were aligned down to the carboxyl terminus and compared with those of papain. The sequence homology between carboxyl-terminal halves of these two thiol proteases of plant origin was found to be 34.7%.

Amino Acid Sequence↗
PubMed1979

A clinical study of tuberculous colitis.

During the last 8 years, the diagnosis of tuberculous colitis has been made radiologically in 22 cases. Twelve were confirmed by histological or bacteriological evidences. The X-ray findings are classified into three types as follows: Type A, showing an extensive scarred area in the ileocecum and ascending colon. Type B, showing annular stricture in the ascending colon and dilatation of its oral side. Type C, showing pouchformation (pseudo-diverticulum) and deformity in the cecum. We emphasize that histological examination and culture of the biopsy specimens by endoscopy were useful for the diagnosis of tuberculous colitis. Furthermore, radiologic changes of the colon by administration of antituberculous drugs were observed.

Adult↗
PubMed1979

Posterior cerebral artery occlusion: clinical, computed tomographic, and angiographic correlation.

The distribution of low-density areas on computed tomography (CT) suggested occlusions in the proximal half of the circummesencephalic portion in 38 patients with posterior cerebral artery (PCA) occlusion. Correlation between clinical, CT, and angiographic findings in 24 cases showed that occlusion was most common in the crural segment. Clinical manifestations and infarction extension varied widely among proximal occlusions. In cases with good collateral filling, the infarction was restricted to the thalamus; in those with poor filling, it involved most of the PCA's territory, and hemorrhagic transformation occasionally ensued. Discrepancies between findings were ascribed to dislodging of emboli or thrombi, recanalization, and transient obscuration of the infarction on CT.

Aged↗
PubMed1979

Anti-allergic activities of a new benzopyranopyridine derivative Y-12,141 in rats.

Passive cutaneous anaphylaxis (PCA) mediated in rats by IgE-like antibodies against egg albumin or the benzylpenicilloyl determinant was inhibited in a dose-dependent manner by intravenous treatment with Y-12,141; the ED50 was 0.09--0.2 mg/kg. The inhibitory effect of Y-12,141 ON PCA was about 5 times as potent as that of disodium cromoglycate (DSCG). Oral treatment with Y-12,141 resulted in the inhibition of PCA, showing an ED50 of 2.5 mg/kg. This action of Y-12,141 on PCA was considered to be due to the inhibition of the release of allergic mediatros from mast cells in a manner similar to DSCG. The results suggest that Y-12,141 may have an anti-allergic activity.

Animals↗
PubMed1979

Effects of 2-[4-(2-imidazo[1,2-a]pyridyl)phenyl] propionic acid (Y-9213) on in vivo release of lysosomal enzymes from rat polymorphonuclear leukocytes during phagocytosis of particles.

Intraperitoneal administration of carrageenin-zymosan suspension into rats induced an increment of the free activity and its ratio of aryl sulfatase in the exudate and such was accompanied by protein exudation and polymorphonuclear leukocyte emigration. Each test solution was orally administered twice, 1 hr before and 2 hr after, or once 2 hr after injection of the irritant. The peritoneal fluid was withdrawn 5 hr after the injection. 2-[4-(2-imidazo[1,2-a]pyridyl)phenyl] propionic acid (Y-9213) and other anti-inflammatory drugs tested inhibited the increment of the ratio of the free activity (aryl sulfatase) in the peritoneal fluid at the same dose which showed anti-inflammatory activities such as prevention of protein exudation. These results suggest that Y-9213 inhibits the phagocytic secretion in vivo of lysosomal constituents from leukocytes.

Animals↗
PubMed1979

Electron microscopic studies on transplantable mucus-secreting and tubular adenocarcinomas of colo-rectal origin in ACI/N rats.

Two transplantable, one mucus-producing (R-1) and the other tubular but less mucinous (R-2), adenocarcinomas were investigated electron microscopically. The R-1 tumor was composed of a large number of intermediate cells and mucus-producing cells, incompletely differentiated goblet-like cells and absorptive-like cells, and a small number of undifferentiated cells. The electron microscopic features of the mucus-producing cells exhibited distinctive features different from those of the epithelium of normal colon. They had highly electron-dense granules, expanded rER, well-developed mitochondria and Golgi apparatus. The R-1 tumor was found to be a well-differentiated adenocarcinoma in agreement with observations by light microscopy, while the R-2 tumor exhibited more malignant features than R-1.

Adenocarcinoma↗
PubMed1979

Denervation-like supersensitivity in the rat vas deferens induced by local application of colchicine to the hypogastric plexus.

In an attempt to induce denervation-like supersensitivity in the smooth muscle of the vas deferens without depriving the tissue of its innervation, a small amount of colchicine was locally applied to the hypogastric plexus of the rat. Colchicine produced supersensitivity and a partial depletion of the tissue norepinephrine. These effects were dose-dependent. The supersensitivity produced by colchicine was qualitatively and quantitatively similar to that induced by denervation. Following application of 20 micrograms of colchicine, the supersensitivity was fully developed by the 4th day. The supersensitivity began to decline by the 6th day but was measurable even after 16 days. Functional neuromuscular transmission was maintained even on the 4th day after colchicine application, although it was significantly less than the control. The time course of the development of supersensitivity was dependent on the length of the adrenergic nerve between the colchicine-applied site and the effector cells. The results indicate that supersensitivity is independent of an irreversible degeneration of the adrenergic nerves. The possible mechanisms of action of colchicine are discussed in relation to the known effects of colchicine on axoplasmic transport.

Animals↗