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Biomedical subjects

K Goto

Publications and source records attributed to K Goto.

At least 757 records · Page 42Linked to original sources
PubMed1988

Pregnancies after co-culture of cumulus cells with bovine embryos derived from in-vitro fertilization of in-vitro matured follicular oocytes.

Bovine follicular oocytes surrounded by cumulus cells for more than one-third of their surface were matured, fertilized and developed in vitro utilizing a co-culture system with bovine cumulus cells. Embryos developed into blastocysts were non-surgically transferred to the uteri of cows at Day 6, 7 or 8 (Day 0 = oestrus). Out of 6 recipient cows (19 blastocysts transferred), 3 became pregnant. One of the 3 pregnant cows carried twins. The results of this study demonstrated the viability of embryos obtained from in-vitro maturation of bovine oocytes followed by in-vitro fertilization and culture to the blastocyst stage in vitro.

Animals↗
PubMed1988

[Testing performance of the improved pinhole collimator and its application to small animals].

Testing performance of the improved pinhole collimator of 1.5 mm aperture attached to the gamma camera was carried out with three kinds of radionuclides, 99mTc, 67Ga and 131I, using a hand-made phantom. The results obtained in this experiment were compared to those obtained with a human pinhole collimator of 4 mm aperture. Those were nearly understood by taking account of the effective aperture of a pinhole collimator for each effective photon energy. And all scintigrams obtained from rats with various scintigraphy showed high resolution images.

Animals↗
PubMed1988

Effects of ethylene glycol-bis-(beta-aminoethyl ether)-N,N'-tetraacetic acid on rabbit sinoatrial node cells treated with cardiotonic steroids.

The effect of ethylene glycol-bis-(beta-aminoethyl ether)-N,N'-tetraacetic acid (EGTA) was studied in rabbit sinoatrial node cells treated with ouabain. When isolated sinoatrial node preparations were bathed in the presence of 10(-5) M ouabain in order to depress electrogenic Na-K pump activity, action potentials ceased rapidly. Thereafter, spontaneous miniature fluctuations of less than 2 mV were observed at the resting potential level, while the membrane potential reached a value of about -31 mV. After exposure to the Ca-free 2 mM EGTA solutions, the miniature fluctuations decreased in frequency as well as in amplitude. However, successive exposure to the Ca-free EGTA solutions produced a short series of slow regenerative potentials. Peak amplitude of the slow regenerative potential increased from reduced miniature fluctuations to a maximum value of 46 +/- 3 mV (mean +/- S.E.; n = 10). Resting potential before initiation of the slow regenerative potential reached a value of about -34 mV. Also, Ca-free perfusion produced a small regenerative potential reaching 19 +/- 2 mV (n = 3). In Ca-free EGTA solution, reduction of external Na produced a decrease in amplitude of the slow regenerative potential when choline chloride or LiCl replaced NaCl. Replacement of 25% of the external Na with choline suppressed the slow regenerative potential. Replacement of 60% of the external Na with Li did not affect the slow regenerative potential. Further reduction of the external Na to 0% of the control condition produced a small regenerative potential. Replacement of Na with Li did not abolish the regenerative potential. In the presence of 4 x 10(-6) M verapamil Ca-free EGTA perfusion did not induce a slow regenerative potential. In the presence of 0.7 x 10(-6) M nifedipine Ca-free EGTA perfusion induced a small regenerative potential reaching 27 +/- 5 (n = 3). For low external pH (6.3) obtained with 5% CO2, the slow regenerative potential induced by Ca-free, EGTA perfusion was suppressed, and the regenerative potential amplitude decreased by about 16 mV. Application of 2.5 mM SrCl2 caused a decrease in slow regenerative potential, and the potential decreased by about 21 mV. The slow regenerative potential is not altered noticeably by 10(-5) M tetrodotoxin.(ABSTRACT TRUNCATED AT 400 WORDS)

Action Potentials↗
PubMed1988

ENG findings in patients with Bell's palsy. Relationship between visual suppression and clinical course.

Electronystagmography (ENG) is known to be a useful method for the evaluation of vestibular disorders. We tested 40 Bell's palsy patients who visited our clinic within 3 weeks after the onset of paralysis with ENG. Regarding the ENG findings and facial nerve damage, there were no correlations between the severity of paralysis in the initial period and the degree of paralysis recovery and existence of spontaneous, positional and positioning nystagmus. Patients with CP had a significantly severe paralysis in the initial period, but then showed varying degrees of recovery from the paralysis. Appearance of DP was not dependent upon the severity of facial paralysis in the initial period or the degree of paralysis recovery. Patients without visual suppression had a more severe paralysis in the initial period and a poor paralysis recovery at 3 months after the onset of paralysis.

Adolescent↗