Biomedical subjects

K Gomi

Publications and source records attributed to K Gomi.

238 records · Page 14Linked to original sources

PubMedDate not supplied

Effect of the type of serum in the medium on sodium ascorbate-induced cytotoxicity.

Sodium ascorbate induced cytotoxicity against human glioblastoma T98G cells in RPMI1640 medium supplemented with fetal bovine serum or human serum samples was studied. Several human serum samples significantly reduced the cytotoxic activity of sodium ascorbate, regardless of sex, age or the disease of the serum donor with or without heat-inactivation of the serum. ESR spectroscopy revealed that this serum effect was not simply due to the alteration of the ascorbyl radical intensity, produced from sodium ascorbate. The present study suggests that the apoptosis-inducing activity of sodium ascorbate might be significantly affected by human serum.

Adult↗

PubMedDate not supplied

Effect of methionine depletion on growth and apoptosis in various tumor cell lines.

Sodium ascorbate, sodium 5,6-benzylidene-L-ascorbate (SBA), gallic acid and caffeic acid induced apoptotic cell death in human myelogenous leukemic cell lines, and stimulated oxidation of methionine into methionine sulfoxide in the culture medium. When various tumor cell lines were cultured in methionine-free medium, their growth was nearly terminated at G1 phase of the cell cycle, producing much smaller number of apoptotic cells. Addition of methionine sulfoxide to the methionine-free medium did not stimulate the apoptosis induction. These data suggest that induction of apoptosis by ascorbates, gallate or by caffeate cannot be simply explained by methionine oxidation.

Antioxidants↗

PubMedDate not supplied

Chelating effect of human serum proteins on metal-catalyzed ascorbate radical generation.

Purified human serum albumin and immunoglobulin G (IgG) were investigated for their metal-chelating activity, using ESR spectroscopy. Both copper (Cu2+) and iron ions (Fe3+) enhanced the radical intensity of both sodium ascorbate and sodium 5,6-benzylidene-L-ascorbate (SBA). Albumin significantly reduced the stimulation effect of copper, but not that of iron. On the other hand, IgG effectively reduced the radical intensity of iron, without affecting that of copper. The present study demonstrates the specific chelating action of these serum proteins, suggesting their possible preventive effects on metal-catalyzed pathogenic diseases.

Adult↗

PubMedDate not supplied

Induction of DNA fragmentation by nicotine in human myelogenous leukemic cell lines.

The effect of nicotine, a component of tobacco, on the cytokine production and cell growth of various cultured cells was investigated. Nicotine did not stimulate, but rather inhibited the tumor necrosis factor production by human peripheral blood mononuclear cells at higher doses. Cytotoxic concentrations of nicotine did not induce the monocytic and granulocytic differentiation of human myelogenous leukemic cell lines (HL-60, ML-1). Nicotine induced internucleosomal DNA cleavage in these leukemic cell lines but not in human peripheral blood lymphocytes and polymorphonuclear cells. The fragmentation-inducing activity of nicotine was not prevented by simultaneous addition of ascorbic acid. Flow cytometry showed a slight increase in the number of G2 + M phase cells, before the appearance of the apoptosis peak. Nicotine transiently increased the intracellular calcium concentration to higher levels in leukemic cells than in normal leukocytes. These data suggest that the induction of DNA fragmentation requires elevation of intracellular calcium concentration above a certain threshold level.

Ascorbic Acid↗