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Biomedical subjects

K Gallo

Publications and source records attributed to K Gallo.

9 recordsLinked to original sources
PubMed1996

The mixed lineage kinase SPRK phosphorylates and activates the stress-activated protein kinase activator, SEK-1.

SPRK (also called PTK-1 and MLK-3), a member of the mixed lineage kinase subfamily of (Ser/Thr) protein kinases, encodes an amino-terminal SH3 domain followed by a kinase catalytic domain, two leucine zippers interrupted by a short spacer, a Rac/Cdc42 binding domain, and a long carboxyl-terminal proline-rich region. We report herein that SPRK activates the stress-activated protein kinases (SAPKs) but not ERK-1 during transient expression in COS cells; the p38 kinase is activated modestly (1.3-2 fold) but consistently. SPRK also activates cotransfected SEK-1/MKK-4, a dual specificity kinase which phosphorylates and activates SAPK. Reciprocally, expression of mutant, inactive SEK-1 inhibits completely the basal and SPRK-activated SAPK activity. Immunoprecipitated recombinant SPRK is able to phosphorylate and activate recombinant SEK-1 in vitro to an extent comparable to that achieved by MEK kinase-1. These results identify SPRK as a candidate upstream activator of the stress-activated protein kinases, acting through the phosphorylation and activation of SEK-1.

Amino Acid Sequence↗
PubMedDate not supplied

Geriatric trauma: a case study.

Major advances in health care have made it possible for a greater number of people to live to advanced age and thereby be exposed to the same risk of injury as the younger segment of the population. Elderly trauma victims, however, possess unique needs that must be addressed. Comorbidity, the aging process, and its relationship to the response to injury, as well as ethical and moral dilemmas such as "do not resuscitate" decisions and cost of care, need to be investigated. Although advanced age clearly predisposes one to an increase in mortality, mortality has also been correlated with complications that develop during the hospital stay. Trauma, especially involving the elderly, is a rich area for nursing research, and a refined knowledge base contributes to improving the quality of trauma care.

Aged↗
PubMedDate not supplied

Emergency department assessment of the adult trauma patient.

Trauma is recognized as a leading killer in the United States today. The development of trauma care systems and traumatology, as a response to this public health problem, has led to the evolution of the role of the trauma nurse. Assessment of the trauma patient is a challenging and demanding role for trauma nurses and is vitally important because the detection of potentially life-threatening injuries and development of standards of care for the resuscitation and management of the trauma patient are based on the initial assessment. The authors address the role of the nurse from preparation for the trauma patient's arrival to continuous monitoring and documentation responsibilities.

Adult↗