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Biomedical subjects

K G Patterson

Publications and source records attributed to K G Patterson.

28 records · Page 2Linked to original sources
PubMed1982

Effect of surgery on monocyte function.

In vitro monocyte lysozyme production (IVMLP) was assessed in 10 male patients preoperatively and at intervals postoperatively. A similar procedure was carried out in 6 sex- and age-matched controls. In contrast to the control group where the IVMLP was constant, the IVLMP levels in the surgical group showed a marked increase at 6-10 h postoperatively, falling to control levels after 10 days. In addition, peripheral monocyte counts were carried out using a Technicon Hemalog D counter which produces accurate reproducible counts for cells such as monocytes which occur at low frequency. The peripheral monocyte counts were stable in the control group, whilst in the surgical patients there were significantly higher readings at 36-48 h, which persisted for up to 10 days compared with preoperative counts. This study suggests that surgery has both a qualitative (IVMLP) and quantitative (monocyte counts) effect on the mononuclear phagocytes. These data may be relevant when considering factors that influence the early stages of wound healing.

Adolescent↗
PubMed1982

Bone marrow processing and cryopreservation.

Three different closed procedures for concentrating bone marrow progenitor cells prior to cryopreservation have been compared. These were by a manual double centrifugation method, a Hemonetics 30 cell separator and an Aminco Celltrifuge. The best results were achieved using the paediatric pheresis set on the Hemonetics model 30. Marrow was frozen in 120 ml aliquots in a programmed freezer with rapid cooling of the freezing chamber during the phase change from the liquid to the solid state. After freeze-thawing the average nucleated cell recovery was approximately 50% and the progenitor cell recovery 80%.

Bone Marrow Cells↗
PubMed1982

Comparative study of the fenwal and pall microaggregate filters used for the production of leucocyte-poor blood.

Eighty units of whole blood in its third week of shelf-life were filtered through the Pall of Fenwal microaggregate filter. Half of these units were centrifuged before filtration. White cell removal was approximately 30% for the Fenwal filter both with and without prior centrifugation; for the Pall filter, removal was 7% without centrifugation and 30% with centrifugation. It is suggested that these simple filters are a useful way of reducing both platelet and white cell content of blood administered to patients requiring multiple transfusion. Their use may delay development of white cell and platelet antibodies obviating the use of washed or frozen cells in these patients.

Blood↗
PubMed1982

Increased neutrophil peroxidase activity in acute myeloid leukaemia.

A case of acute myeloid leukaemia (AML) (M2 type) is described in which the neutrophil population contained markedly increased peroxidase activity. The increased peroxidase activity was initially detected during routine counting with the Hemalog D automated differential counter and the increased enzyme staining was confirmed by manual and ultrastructural cytochemistry. The abnormal neutrophil population persisted after blast cells had disappeared from the blood and until marrow hypoplasia was induced by chemotherapy. When remission emerged, the peroxidase activity of the neutrophil population was normal. At subsequent relapse, the neutrophil population with increased peroxidase activity reappeared and with the induction of second remission neutrophil peroxidase again returned to normal. In 8 other cases where sufficient neutrophils were present for analysis by the Hemalog D, neutrophil peroxidase was normal or not increased in 7 cases, but was markedly reduced in 1 case of AML (M6 type). It is suggested that abnormalities of neutrophil peroxidase activity detected by the Hemalog D may serve as useful leukaemic markers.

Bone Marrow↗
PubMed1982

Haematological aspects of systemic lupus erythematosus: a reappraisal using automated methods.

Twenty-two haematological parameters were measured in 30 patients with systemic lupus erythematosus (SLE) at 110 patient attendances. Using a 10,000 cells per sample automated differential counter, the major abnormalities demonstrated were: lymphopenia, monocytopenia, eosinophilopenia (each of which showed strong correlation with steroid therapy) and increased numbers of cells of high peroxidase activity. Despite the common lymphocytopenia elevation of large unstained cells was noted in 20% of patients. However, no patient with severe disease had a lymphocyte count above 1.9 X 10(9)/l or a haemoglobin above 11.7 g/dl.

Basophils↗
PubMed1981

A hemalog D analysis of chronic lymphocytic leukaemia and other lymphoproliferative disorders affecting the blood.

A correlative clinical and Hemalog D study of 80 cases of chronic lymphocytic leukaemia (CLL) was performed and the results compared with those in other lymphoproliferative disorders affecting the blood (eight cases of hairy-cell leukaemia, HCL; three cases of prolymphocytic leukaemia, PLL: two cases of lymphoplasmacytoid proliferation, LPP; and one case of Sézary's syndrome, SS). In CLL the mean percentage and absolute counts of large unstained cells (LUC) were 8.7% and 6.2 x 109/1 (6200/mm3), and the percentage of LUCs was not substantially affected by treatment; there was no correlation between LUCs and Rai clinical stage. SS and LPP were characterised by a similar percentage of LUCs, but both HCL and PLL were distinguished by a higher mean percentage of LUCs. Stage IV CLL was associated with monocytopenia, but other stages of CLL, PLL, LPP, and SS showed no consistent reduction of absolute numbers of non-lymphoid cell types. In contrast, HCL was characterised by a consistent absolute neutropenia and monocytopenia which distinguished its Hemalog D profile from that of PLL.

Humans↗
PubMedDate not supplied

Widespread bone disease in acute myeloid leukaemia.

We describe a 11-year-old boy with acute myeloid leukaemia who presented with widespread bone disease. Spine X-rays revealed multiple crush fractures and there were multiple hot spots on the bone scan. The bone-mineral density was markedly reduced but there was no hypercalcaemia or hypercalcuria. Bone marrow aspirate revealed 98% blast cells and a balanced translocation between chromosomes 10 and 17 in seven of nine metaphases. Plasma interleukin-6 level before chemotherapy was high at 53 pg/ml. We postulate that the mechanism for bony destruction in this case was similar to that in the adult disease myeloma.

Bone Density↗