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Biomedical subjects

K G Henriksson

Publications and source records attributed to K G Henriksson.

At least 73 records · Page 4Linked to original sources
PubMed1986

Muscle biopsy in primary fibromyalgia. Light-microscopical and histochemical findings.

Seventy-seven muscle biopsies from 57 patients with primary fibromyalgia and 17 biopsies from 9 healthy controls were examined by routine histopathological and histochemical methods. All patients fulfilled strict diagnostic criteria. 42 biopsies from patients were deemed normal or borderline, while 35 showed discrete pathological changes (degeneration, regeneration, inflammatory infiltrates, ragged red fibres, and 'moth-eaten' fibres in muscles other than m. trapezius). Biopsies from controls were normal or borderline in 14 and showed mild focal abnormalites in 3 cases. 'Moth-eaten' fibres were found in 35 of 41 trapezius biopsies taken from patients and in 9 of 10 trapezius biopsies from controls. Ragged red fibres were found in 15 of 41 trapezius biopsies taken from patients. In the deltoid muscle, 'moth-eaten' fibres were found in 3 and ragged red fibres in 2 of 9 biopsies from patients. Frequency of type I and type II fibres and the area of muscle fibres were the same in patients as in controls. Capillary density was the same in both groups. These changes, although not sufficient to constitute a diagnostic test for PF, indicate that this condition has an organic basis.

Adult↗
PubMed1985

HLA-DR expression, T lymphocyte phenotypes, OKM1 and OKT9 reactive cells in inflammatory myopathy.

Frozen muscle biopsy sections from 13 patients with inflammatory myopathy and from four healthy volunteers were characterized with a double immunohistochemical staining technique, utilizing a panel of antisera to cell surface antigens. The technique enables simultaneous visualization of HLA-DR reactive cells and other cell types, i.e., T lymphocytes. In inflammatory myopathy, large numbers of HLA-DR reactive "macrophage/dendritic" cells were shown. Almost all other inflammatory cells and endothelial cells also expressed HLA-DR, as did the sarcolemma in the vicinity of inflammatory foci. Large numbers of T lymphocytes were detected by alphaLeu-1 antibodies. In most instances, Leu-3a reactive T "helper" lymphocytes dominated over alphaLeu-2a reactive T "suppressor/cytotoxic" lymphocytes. Many T lymphocytes of both phenotypes appeared in close contact with HLA-DR expressing non-T cells. OKM1 and OKT9 antibodies, labeling macrophages and "proliferating" cells, respectively, were common among inflammatory cells. The findings provide basic data that are important for the understanding of inflammatory myopathy.

Adolescent↗
PubMed1985

Changes in histochemical profile of muscle after long-term electrical stimulation in patients with idiopathic scoliosis.

Adolescent patients with idiopathic scoliosis were treated with long-term electrical stimulation (30 Hz) at the posterior axillary line on the convex side of the curvature in order to correct the spinal deformity. The patients were also followed with muscle biopsies from the latissimus dorsi of the stimulated side taken before, after 3 and 6 months of electrical stimulation. There was a tendency for an increase in the percentage of type I and especially the type II C (undifferentiated) fibers after stimulation. The mean muscle fiber area and the fiber areas of the various fiber types did not change significantly. Histopathological findings were generally rare before as well as after 3 months of electrical stimulation, the only noticeable finding being a somewhat increased frequency of atrophic fibers in groups after 6 months of stimulation. In all studied patients the enzymatic activity of citrate synthase increased after 3 months and further in three studied patients after 6 months of stimulation. The present study gives some evidence of an adaptive process caused by electrical stimulation towards a more fatigue-resistant muscle.

Adenylate Kinase↗
PubMed1984

Impaired muscle function with aging. A background factor in the incidence of fractures of the proximal end of the femur.

Muscle biopsy material from vastus lateralis musculus quadriceps was obtained during surgery from 43 66-100-year-old female and nine 70-89-year-old male patients with fresh hip fractures. Before the accident, 18 women and two men were clinically healthy, and in the others, one-third had cardiovascular, one-third locomotor, and one-third neurologic disorders. Twelve women lived in institutions. The other women and all of the men lived in their own homes. A striking finding was a more advanced reduction in muscle fiber size in the vastus lateralis, especially in the fast twitch (FT) fibers. The reduction occurred in preaccident clinically healthy fracture patients. With advancing age, the proportions of the slow twitch (ST) and FT fibers remained the same. Well-maintained, enzymatic metabolic capacity, moderate neuropathic changes, and absence of myopathic changes indicate that the quality of the muscle tissue is relatively well preserved, even in elderly patients with hip fractures. Muscle fiber atrophy and weakness in muscle strength may be attributable in part to a low physical activity and are possibly reversible with exercise in some individuals.

Adenylate Kinase↗
PubMed1983

Early infant death in nemaline (rod) myopathy.

Congenital 'floppy infant' syndrome with very early death is uncommon. It is here described in a girl. Histopathological examination of a muscle biopsy at the age of 10 days showed rods and infiltration of inflammatory cells. Death occurred at 5 weeks of age. Electron microscopy of the necropsy material showed widened Z disks and rods of Z disk density with a typical transverse periodicity of 18-20 nm. The differential diagnosis between nemaline (rod) myopathy and infantile polymyositis is discussed. Nemaline (rod) myopathy should always be considered in cases of the congenital 'floppy infant' syndrome.

Female↗
PubMed1983

Metabolic changes in a case of malignant hyperthermia.

A patient with fulminant MH is presented. The metabolic changes in muscle tissue, whole blood and plasma are compared with those obtained from the porcine model. The samples were taken 1 h after treatment (including dantrolene sodium) had been commenced. Increased concentrations of lactate, pyruvate and citrate were found in muscle, whereas the concentrations of potassium, free inorganic phosphate and glycogen were reduced. The ATP, ADP and phosphoryl creatine concentrations remained unchanged. In blood, increased concentrations of alanine, lactate and glycerol were observed. An increase in carbon dioxide production was found. These changes are similar to those found in the porcine model.

Carbon Dioxide↗
PubMed1983

Direct tissue isoelectric focusing of nervous system and muscle sections for detection of IgG patterns.

Agarose isoelectric focusing (IF) of 5-70 microns sections of frozen autopsy material (direct tissue IF; DTIF) from the central nervous system (CNS) and peripheral nervous system (PNS), and from muscle biopsy, followed by immunofixation with unlabeled or radiolabeled anti-serum against human gamma chains or IgG Fc fragments has been developed for analysis of IgG patterns. The DTIF technique followed by antiserum immunofixation is a promising tool for characterization of proteins in autopsy and biopsy materials from nervous and muscular systems. In multiple sclerosis brains, oligoclonal IgG bands were found which differed regarding number and mobility between the brains, and also between two different regions in single brains. For PNS, IgG patterns were easily detectable. In polymyositis muscle, no difference was found for IgG patterns when compared with normal muscle or serum.

Humans↗
PubMed1983

Fetal muscle biopsy.

Fetal muscle specimens suitable for histological and histochemical studies were obtained under fetoscopic visualization by removing a small piece of skin, thereby exposing the underlying muscle. The procedure was carried out in 7 cases prior to second-trimester abortion. Muscle specimens so obtained may become useful in the prenatal diagnosis of Duchenne muscular dystrophy.

Female↗
PubMed1982

Quantitative electromyography in polymyositis and dermatomyositis.

Twelve patients with polymyositis or dermatomyositis were examined repeatedly during the course of the illness by muscle strength tests, serum enzyme determinations, frequency analysis of the electromyographic signal, and estimations of the spontaneous activity. All methods can be used to follow the course of the disease. Increasing muscle strength was correlated to decreasing serum enzyme values, decreasing spontaneous activity, and decreasing proportion of high-frequency components in the EMG signal, except during the initial period of treatment, when a temporary increase in high-frequency components was recorded.

Adolescent↗
PubMed1982

Polymyositis--treatment and prognosis. A study of 107 patients.

One hundred and seven patients with polymyositis or dermatomyositis were followed for an average of 5 years. In 100 patients it was possible to evaluate the effect of treatment with steroids or a combination of steroids and immunosuppressive drugs. Fifty patients improved, 50 did not. Compared with the treatment-resistant group the treatment-responsive patients were of younger age, did not have an associated malignancy or cardiac disease and began treatment within 24 months after the onset of muscular weakness. In the treatment-responsive group the degree of improvement was correlated to the mean dose of steroids given during the first 3 months of treatment. Eighty-seven per cent of surviving responsive patients had no or slight disability at the end of the investigation. The mortality rate was 23% and was highest in the treatment-resistant group. Comparison between different treatment programs was possible and based on the results, a treatment schedule could be recommended. The principles of this schedule are: During the first month high doses of prednisone or prednisolone should be given daily. From the second month, an alternate-day administration can be employed. Tapering to maintenance dose should be gradual and slow. The treatment period should be long. The mean duration in the present series was 27 months.

Adolescent↗
PubMed1982

Quantitative analysis of muscle biopsies from volunteers and patients with neuromuscular disorders. A comparison between estimation and measuring.

106 muscle biopsies from patients with neuromuscular diseases or symptoms were assessed by routine inspection and quantitative examination. Eighty-six specimens were classed as pathological on routine evaluation and 84 on quantitative examination. Eleven specimens that were pathological on routine evaluation were "missed' by quantitative examination. Nine judged normal on routine examination were pathological according to quantitative criteria. Relative measures including the coefficient of variance, the difference between type I and type II fibres, and the difference between fascicles proved valuable for distinguishing between normal and pathological specimens. Quantitative analysis is a valuable complementary method which improves the detection of pathological states in muscle.

Adolescent↗
PubMed1980

The distribution of intermediate filament protein (skeletin) in normal and diseased human skeletal muscle--an immunohistochemical and electron-microscopic study.

The presence of intermediate filaments in various mature and immature eukaryotic cells is well documented. By using antibodies against the intermediate (skeletin) filaments of cow heart Purkinje fibres, we have in the present work analyzed the distribution of skeletin in normal and diseased skeletal muscle fibres. Antiskeletin proved to be a useful tool for the identification of regenerating fibres in Duchenne muscular dystrophy. In mature muscle fibres with well-preserved myofibrils, skeletin had an intermyofibrillar localization at the Z-disk level where intermediate filaments were demonstrated. The presence of skeletin was also demonstrated around the rods of muscle fibres in nemaline myopathy biopsies, but the rods themselves did not show any cross-reactivity. This report exemplifies the usefulness of antibodies in muscle pathology.

Adolescent↗
PubMed1980

Muscle histochemistry and muscle function.

A short review of the histochemical characteristics of the different muscle fibre types is given. The possibility of changing contraction time, strength, and endurance by physical training is discussed.

Adenosine Triphosphatases↗
PubMed1979

Eosinophilic fasciitis. Review and report of six cases.

Six patients with eosinophilic fasciitis are presented. This syndrome is characterized by indurative swellings of arms and legs, with rapidly progressing difficulties in extending elbows, wrists, and fingers, and often limited motion of shoulders and ankle joints. Pain when contracting muscles, and weakness of proximal muscles and hand grip are common features. The frequent occurrence of localized skin lesions has presented differential diagnostic difficulties to systemic sclerosis and to polymyositis. Visceral involvement and Raynaud's phenomena, however, are absent or mild. Blood eosinophilia, hypergammaglobulinemia, and unspecific signs of inflammation are found. Biopsy of muscle fascia gives characteristic histopathological findings of cell infiltrations (mostly mononuclear cells, frequently eosinophils) and vascular proliferation, in the middle layer of a thickened fascia. Skin changes are prevalent, but not conclusive for the diagnosis, and myositis in some patients might be difficult to distinguish from polymyositis. The importance of the clinical recognition of eosinophilic fasciitis and the inclusion of fascia in diagnostic muscle biopsies, is underlined.

Adult↗