Search PubMed⌕ Search

Biomedical subjects

K Fukushi

Publications and source records attributed to K Fukushi.

At least 91 records · Page 5Linked to original sources
PubMed1983

[Autopsy case of adult T cell leukemia associated with hypertrophy of the parathyroid glands].

An autopsy case of adult T cell leukemia associated with hypercalcemia and hyperplastic parathyroid glands is reported. A 51-year-old man complained of systemic lymphadenopathy and hepato-splenomegaly; a diagnosis of adult T cell leukemia was made based on clinico-pathological findings. The pathogenesis of hypercalcemia in adult T cell leukemia is discussed. However, cases associated with hyperplasia of the parathyroid gland are very rare. We emphasize the role of the parathyroid gland in the pathogenesis of hypercalcemia in adult T cell leukemia.

Cell Division↗
PubMed1982

Synthesis of 18F-6-fluoropurine and 18F-6-fluoro-9-beta-d-ribofuranosylpurine.

Introduction of fluorine-18 into the 6-position of purines is described. 18F-6-fluoropurine and 18F-6-fluoro-9-beta-D-ribofuranosylpurine were prepared with high radiochemical yields by nucleophilic displacement of the trimethylammonio-group of purine with 18F-fluoride under mild conditions. 18F-labeling conditions such as reaction temperature and time, addition of crown ether and dose of a substrate have been studied. Under adequate conditions, yields of about 38% in the case of free base and about 63% in the case of ribofuranosyl derivative have been obtained. The latter compound has also been prepared in a non-carrier-added state. The stability of 18F-purines was examined in Tris buffer at 37 degrees.

Fluorouracil↗
PubMed1982

Preparation of 18F-labeled 6- and 2-fluoro-9-benzylpurine as a potential brain-scanning agent.

The preparations of [18F]-6-fluoro-9-benzylpurine(I) and [18F]-2-fluoro-9-benzylpurine(II) are described. (I) was prepared by two methods: (i) halogen exchange of 6-chloro-9-benzylpurine with Ag 18F; and (ii) displacement of trimethylpurin-6-ylammonium chloride with K 18F, followed by 9N benzylation. The latter labeling method was far superior to the former, both in radiochemical yield and in specific activity. (I) was relatively stable in a Tris-HCl buffer (0.4 M, pH 7.6) at 37 degrees C, but was easily hydrolyzed in 1 N HCl. Biodistribution of (I) in mice demonstrated high uptake in the brain. (I) can be expected to be a potential brain-scanning radiopharmaceutical for positron computed tomography.

Animals↗
PubMed1982

Aspects of the preparation of 18F-2-deoxy-2-fluoro-D-glucose (18FDG) for medical use.

Fluorine-18-2-deoxy-2-fluoro-D-glucose (18FDG) for medical use had been prepared with the attention regarding radiochemical yield and purity, specific activity and quality control. More than four 18F-by-products in 18F-adducts from the reaction of triacetyl glucal with 18F2 were detected by the autoradiography of thin layer plates in which two by-products could not be perfectly removed by a column chromatography, and selective collection of the eluate gave 18FDG with the purity of 96.7% as an average in 12 runs. At end of synthesis (EOS) 259-925 MB (7-25 mCi) of a sterilized, isotonic solution of 18FDG was obtained with the specific activity of 629-851 MBq (17-23 mCi)/mg at end of bombardment (EOB) after preparation time of 3-3.5 hr, in which bacteria and pyrogen were not detected.

Chromatography↗
PubMed1982

[Clinical evaluation of cefotiam in internal medicine].

Cefotiam (CTM) was administered to 52 patients with infectious disease associated with respiratory system, hematological malignancy, urinary system and other system. Good clinical responses were obtained in 38 out of 52 cases (73.1%). Neither objective and subjective side effects nor extreme abnormalities of laboratory tests were observed in these patients. It can be, therefore, concluded that CTM is 1 of the most useful drugs for infectious diseases in respiratory system, hematological malignancy, urinary system and other system.

Adolescent↗
PubMed1981

Crystallization and properties of creatine kinase from equine skeletal muscle.

A crystalline creatine kinase was obtained from equine skeletal muscle. The enzyme was homogeneous, as judged by ultracentrifugation and disc electrophoresis on polyacrylamide gel. The crystalline enzyme had a specific activity of 110 units per mg of protein, that is, 14-fold purification over the crude extract of equine skeletal muscle. The molecular weight of the enzyme was determined to be 84,600 by the conventional low-speed sedimentation equilibrium method, and s020,w was 5.32S. Eight cysteine residues were found on amino acid analysis, two of which were essential for the enzymatic activity.

Amides↗
PubMed1981

Dysplasia and reserve cell hyperplasia-like change in human esophagus.

The esophagus was totally examined in 264 autopsied cases and 61 operated cases, for a total of 325 cases, to clarify the histogenesis of squamous cell carcinoma of the esophagus. Epithelial dysplasia of the mucosa was present in 27% and subclinical carcinoma was found in 2.4%. Hyperplasia of the duct of the esophageal gland proper was present in 34% and cysplasia of the ductal epithelium in 3%. Reserve cell hyperplasia-like change of the islet of the ectopic gastric mucosa was found in 4% and reserve cell hyperplasia-like change of the esophagogastric junction zone in 13%. Of the seven cases of microcarcinoma, two showed dysplasia and gradual transition and one presented dysplasia and abrupt transition. Another two were considered to have originated in the ductal epithelium. These findings suggested that they could all be the sites of origin of cancer development.

Adult↗
PubMed1980

[Clinical study of adrenal imaging with 123I-labeled-6 beta-iodomethyl-19-norcholesterol (author's transl)].

Although adrenal scintigraphy with 131I-6 beta-iodomethyl-19-norcholesterol (NCL-6-131I) was used for the first noninvasive imaging of the adrenal glands, it has the disadvantages. These are: (i) relatively high radiation dose to patients, (ii) the long time required for completion of the scan. To solve these problems, 123I-labeled 6 beta-iodomethyl-19-norcholesterol (NCL-6-123I) was synthesized and was used for adrenal scintigraphy in 6 cases. Successful images were obtained with 185 MBq (5 mCi)-260 MBq (7 mCi) of NCL-6-123I, and optimal time required for the satisfactory scan was 2-3 days after the administration. The estimate radiation dose was reduced by using 185 MBq (5 mCi) of NCL-6-123I instead of 18.5 MBq (0.5 mCi) of NCL-6-131I. From the result of this clinical study, NCL-6-123I was preferred for the adrenal scintigraphy.

Adosterol↗