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Biomedical subjects

K Fujinaga

Publications and source records attributed to K Fujinaga.

At least 163 records · Page 9Linked to original sources
PubMed1985

Subtypes of herpes simplex virus type 1 in Japan: classification by restriction endonucleases and analysis of distribution.

An attempt was made to classify herpes simplex virus type 1 (HSV-1) isolates into subtypes on the basis of the combination of the gain or loss of specific cleavage sites of HSV-1 genomes with each of three restriction endonucleases (Bam HI, Kpn I, and Sal I). According to the criteria we used for the determination of HSV-1 subtypes, 93 strains of HSV-1 that were isolated in three areas of Japan (Sapporo, Tottori, and Kagawa) were tentatively classified into eight subtypes: subtypes A-H. The bulk of the strains (84 of 93) fell into three subtypes: A, C, and H. There were highly significant differences (P less than .01) in the proportion of subtypes A and H that were isolated in Sapporo as compared with those isolated in Tottori and in Kagawa, which are geographically far from Sapporo. No significant differences, however, were found in subtypes isolated in Tottori as compared with those isolated in Kagawa, which are geographically close to each other. These data suggest that there might be a correlation between the genome structure of HSV-1 and the areas of their isolation in Japan.

Child↗
PubMed1985

Epidermodysplasia verruciformis associated with Bowen's carcinoma, B lymphocytopenia and decreased immune functions.

A case of epidermodysplasia verruciformis associated with Bowen's carcinoma, persistent B lymphocytopenia and decreased immune functions is reported. Human papilloma virus 5 (HPV-5) DNA was shown to be associated with the DNA from the tumor tissue by Southern blot hybridization using P-labeled HPV DNA sequences cloned on plasmid vectors. The associated Bowen's carcinoma in our case may be caused by multiple-factor relationships which include (a) an oncogenic potential of infected virus; (b) an inherited abnormality in immune function, and (c) the decreased immune function resulting from the viral infection itself. A marked B lymphocytopenia appears to be associated with persistent viral infection.

Aged↗
PubMed1985

Genome type analysis of adenovirus type 4 isolates, recently obtained from clinically different syndromes in some areas in Japan.

DNA cleavage analyses with EcoRI, HindIII and BamHI were carried out to investigate genome types of recent Ad 4 isolates obtained from acute respiratory disease (8 strains), and ocular disease (11 strains) in Japan. DNA cleavage patterns of all 19 isolates studied were identical regardless of whether they were recovered from respiratory tract or conjunctiva, but were distinct from that of the prototype strain.

Adenoviridae Infections↗
PubMed1985

Two distinctive transforming DNA regions on the canine adenovirus type 1 genome.

By transfecting cloned DNA fragments of canine adenovirus type 1, a novel transforming region of mammalian adenovirus was demonstrated inside the viral genome. Besides the left-end SmaI-D fragment (left 17.2%), an internally located SmaI-E fragment (17.2-28.7%) of canine adenovirus type 1 was found to transform primary rat kidney cells.

Adenoviruses, Canine↗
PubMed1984

Restriction endonuclease cleavage analysis of herpes simplex virus isolates obtained from three pairs of siblings.

Cleavage of herpes simplex virus type 1-infected cell DNA with restriction endonucleases showed that profiles of paired isolates obtained from three groups of siblings were essentially identical. This finding is different from that for profiles of other paired isolates that are epidemiologically unrelated. One set of paired isolates of the three had completely identical profiles. However, two other sets of paired isolates yielded minor heterogeneity in fragments derived from the regions spanning the unique repeat junction and repeated regions of the herpes simplex virus type 1 genome. This suggests that the regions of the herpes simplex virus genome are variable during viral DNA replication. This finding also supports the hypothesis that the same variants tend to be transmitted and thereafter perpetuate within a family cluster.

Child↗
PubMed1984

Molecular epidemiology of adenovirus type 8 (Ad 8) in Taiwan: four subtypes recovered during the period of 1980-1981 from patients with epidemic keratoconjunctivitis.

Adenovirus type 8 (Ad 8) has been the major and important causative agent of epidemic keratoconjunctivitis (EKC). By enzymatic cleavage analysis with five endonucleases, PstI, HindIII, BamHI, SalI and SstI, 27 out of 149 Ad 8 isolates recovered from patients with EKC during the period from July, 1980 to July, 1981 in Kaohsiung, Taiwan, were studied. By cleavage patterns, 27 Ad 8 isolates in Kaohsiung were classified into four subtypes which were found to be different from the subtypes (Ad 8A, Ad 8B) prevalent in Sapporo (1).

Adenoviruses, Human↗
PubMed1983

Outbreak of infantile gastroenteritis due to type 40 adenovirus.

Genetic and antigenic characterisation was performed on a strain of adenovirus (EAd) isolated from an outbreak of gastroenteritis which occurred in an orphanage in the City of Sapporo, in the room housing the eldest children, who ranged in age from 14 to 22 months. 7 of the 11 children housed in that room had diarrhoea between July 11 and July 22, 1982. All 7 shed adenoviruses detectable by electron microscopy in their stools. Immune electron microscopy showed that all patients as well as the healthy contacts sharing the room underwent seroconversion to EAd. There was no homology, or very slight homology, between DNA of EAd and those of adenoviruses belonging to subgroups A to E. Antigenically EAd was closely related to type 40 adenovirus, so far the sole member of the newly identified subgroup F. This outbreak of gastroenteritis is the first in which the causative agent has been identified as being a member of subgroup F adenoviruses.

Adenoviridae Infections↗
PubMed1983

Hinf family: a novel repeated DNA family of the human genome.

The isolation of a mutant adenovirus carrying an insertion of cellular DNA has led to the identification of a new family of human repetitive sequences, which are found tandemly arranged in the genome. The sequence of the viral insert resembles that of eukaryotic transposable elements.

Adenoviruses, Human↗
PubMed1983

Analysis of retinoblastoma for human adenovirus type 12 genome.

Adenovirus type 12 has high oncogenic potential in newborn rodents. Moreover, adenovirus 12 induces retinoblastoma-like tumours in baboons and transforms in vitro human embryo retinoblasts. Since adenovirus-transformed cells contain adenovirus transforming gene sequences, the detection of adenovirus 12 transforming gene in tumour cell DNA can provide evidence for or against a possible aetiological role of adenovirus 12 in retinoblastoma. In this experiment, cell DNAs from six retinoblastomas were assayed for adenovirus 12 transforming gene sequences by spot hybridization and Southern blot hybridization, using the labelled EcoRI-C fragment of adenovirus 12 DNA as a probe (the far left 16.5% of the viral genome). No adenovirus 12 transforming gene sequences were detected at the level of 0.1 or 0.5 copy of the probe per diploid cell DNA in all of six retinoblastomas.

Adenoviruses, Human↗
PubMed1983

Comparative sequence analysis of the inverted terminal repetition in the genomes of animal and avian adenoviruses.

The nucleotide sequences at the inverted terminal repetitions from two animal adenoviruses, infectious canine hepatitis virus and equine adenovirus, and from one avian adenovirus, CELO, were analyzed. DNAs from infectious canine hepatitis virus and equine adenovirus contain a homologous region which is 23 nucleotides long from the terminus. The first 17 nucleotides of this region are identical to the ones in human adenovirus type 2 DNA. The striking homologous sequence of 14 nucleotides, conserved in the inverted terminal repetitions of several human adenovirus strains and in simian adenovirus type 7, is only partially conserved in the two animal and one avian adenoviruses reported here.

Adenoviridae↗
PubMed1983

Integration and methylation of shope papilloma virus DNA in the transplantable Vx2 and Vx7 rabbit carcinomas.

The Shope papilloma virus (SPV) DNA present in SPV-induced benign and malignant rabbit tumors, particularly in the transplantable carcinoma Vx2 and Vx7, was examined with regard to physical states and extent of methylation. Vx2 and Vx7 carcinomas contained 10-22 viral genomes per diploid cell, and domestic and cottontail rabbit papillomas 40-400 and 1000-8000, respectively. The digestion of Vx2 and Vx7 DNA with the restriction enzyme KpnI, which does not cleave SPV DNA, yielded a single virus-specific DNA band about nine times larger than the genome length, but EcoRI, which cuts the circular SPV DNA once, cleaved this DNA to the genome-size fragments. However, three or four weak bands which may contain viral segments linked to cellular sequences were also identified, and at least two were shared by both Vx2 and Vx7 carcinomas. The analysis with a set of MspI and HpaII, which discriminates the methylated DNA sequence -CC*GG-, showed that 10-40% of the sites of viral DNA are methylated in papillomas, 30-80% in primary carcinomas, and more than 90% in the transplantable carcinomas.

Animals↗
PubMed1983

Restriction endonuclease cleavage analysis of adenovirus type 8: two new subtypes from patients with epidemic keratoconjunctivitis in Sapporo, Japan.

We studied the restriction endonuclease cleavage patterns of DNAs of adenovirus type 8 (Ad 8) isolated from epidemic keratoconjunctivitis cases. DNAs of 25 Ad 8 isolates collected during the period from 1975 to 1981 in Sapporo were subjected to enzymatic cleavage with PstI, BamHl , HindIII and SalI. On the basis of the cleavage patterns, the isolates were divided into two subtypes, A and B, both different from Trim strain used as the Ad 8 prototype strain. Subtype A was prevalent in the period from 1975 to 1978, while subtype B in the period from 1976 to 1981.

Adenoviridae Infections↗
PubMed1983

Establishment and characterization of rat cell lines transformed by the left-end DNA fragments of adenovirus type 31.

A rat cell line, 3Y1, was transfected with the left-end DNA fragments of adenovirus (Ad) 31 DNA and transformed cell lines were established. A cell line 31BY4-1, which was induced by Ad31 BamHI-B (left-most 17.4% of the genome), showed typical transformation phenotypes, while cell lines 31GY1-2, 31GY2-5, and 31GY3-4, which were induced by Ad31 HindIII-G (left-most 6.7% of the genome), showed intermediate phenotypes between transformed and untransformed cells, with the following properties: (1) percent plating efficiencies in Eagle's minimum essential medium with 2% fetal calf serum and in soft-agar culture were extremely low, like those of 3Y1 cells; (2) they form tumors in newborn rats only after long latent periods. Southern blot hybridization revealed that, in all the transformed cell lines, viral DNA sequences were integrated at multiple loci in large-molecular-weight cell DNAs. Northern blot hybridization showed that viral mRNAs of early region 1A (E1A) and early region 1B (E1B) were both transcribed in 31BY4-1 cells as well as in KB cells early after infection with Ad31. On the other hand, E1B viral mRNA was present in a much lesser amount in 31GY cell lines, or was undetectable.

Adenoviridae↗