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Biomedical subjects

K Fisher

Publications and source records attributed to K Fisher.

100 records · Page 6Linked to original sources
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Cryopreservation of Mediterranean fruit fly embryos.

In this paper we present a procedure to cryopreserve the embryos of a tephritid, the Mediterranean fruit fly (Ceratitis capitata), by vitrification. Developmental stages between 24 and 32 hours after oviposition were examined for tolerance to cryopreservation. Embryos, 27-hr-old and incubated at 29 C, were found to be at the most suitable stage for treatment. Effects of the previtrification steps of our protocol, dechorionation, permeabilization, cryoprotectant loading, and dehydration, on survival to hatching were also assessed. Dechorionation did not affect viability, while isopropanol and a hexane treatment used in the permeabilization step of the protocol reduced hatching by about 15%. This reduction was dependent on the amount of isopropyl alcohol carried over into the hexane rinse. The remaining previtrification steps reduced hatching by an additional 10%. After optimization of the procedure, normalized hatching was 44% after vitrification in liquid nitrogen vapor followed by storage under liquid nitrogen for a test period of 7 days. Post cryopreservation larval diets containing wheat bran, corncob grits, or agar as the base were examined for survival to pupation and emergence. A yield of 34% egg to adult emergence was obtained when the agar-based diet was used for rearing larvae that had experienced cryopreservation during the embryonic stage.

Animals↗
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The diagnosis of dementia associated with alcoholism: a preliminary report of a new approach.

The historical, clinical, neurological and neuropsychological features of 13 subjects with independently diagnosed dementia associated with alcoholism (AlcD) were compared to 13 subjects with probable Alzheimer's disease (AD), matched for age and severity of dementia. Neurological abnormalities were present in all the subjects with AlcD even though the diagnosis of Wernicke-Korsakoff was recorded in only one of these subjects. Only one subject with probable AD demonstrated any neurological abnormality. There was no difference between the AD and AlcD subjects in either the total scores of the Mini-Mental State Examination (MMSE) or in any of the subscores. The presence of neurological signs does appear to be a useful method to assist in the diagnosis of AlcD.

Aged↗
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Evaluation of quick medical reference (QMR) as a teaching tool.

To determine whether the Quick Medical Reference (QMR) program can improve diagnosis or enhance learning among internal medicine residents, we compared the diagnostic accuracy of the program with that of residents at various training levels. The cases were from a prospective convenience sample of 40 patients admitted by 10 first-year residents (interns) and two chief medical residents. Four sets of differential diagnoses were created for each case--the first set by an intern, the second set by a chief resident, and the third and fourth sets by QMR, using the findings of the interns and chief residents, respectively. The diagnostic accuracy of the interns and chief residents was significantly greater than that of QMR. However, the chief residents indicated that QMR did increase their understanding of disease processes and offered educational value.

Computer-Assisted Instruction↗