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Biomedical subjects

K Filipiak

Publications and source records attributed to K Filipiak.

At least 19 recordsLinked to original sources
PubMed2000

Cell cycle and apoptosis in tumours of oral cavity estimated by image cytometry.

The aim of investigations was to examine whether diploid and aneuploid tumours of oral cavity differ in frequencies of occurrence of individual phases of cell cycle and of apoptosis. Investigations were carried out on 53 tumours using techniques of image cytometry. The studies were performed on properly isolated cells of tumours, which were stained using Feulgen method and analysed by image cytometry. Using a suitable computer programme, the percentages of cells present in individual phases of cell cycle and in apoptosis were estimated. No respective differences could be disclosed between diploid and aneuploid tumours. In addition, the ratios of the fraction of cells in S phase of the cell cycle and those in apoptosis were calculated and were found to carry certain prognostic significance in patients with squamous cell carcinomas of oral cavity.

Aneuploidy↗
PubMed1999

[Compensatory mechanisms of food transmission and lower respiratory tract protection after classical and extended supraglottic laryngectomies. Computerized topokinetic analysis of roentgen-cinematographic images].

Studies were performed on 22 patients, aged from 17 to 78 years, in whom, owing to laryngeal cancers, partly classical or extended supraglottic laryngectomy was carried out. The evaluation involving the pharyngeal deglutition course was accomplished by resorting to computerized topokinetic analysis of the roentgen-cinematographic images. The completed observation revealed good mobility of the anatomical structures participating in deglutition, small volume and number of glossolaryngeal recesses. There were few patients in whom the contrast medium passed to the trachea. Better passage of food as well as protection of lower respiratory tract were associated with the improvement of parameters facilitating the deglutition.

Adaptation, Physiological↗
PubMed1998

[Immuno-endocrine interactions in the testis: possible involvement of Ca2+ in signal transduction pathway in Leydig cells].

The testis is divided, both functionally and anatomically, into two specialised compartments, i.e. the vascular interstitial tissue and avascular seminiferous tubules. Leydig cell steroidogenesis is mainly controlled by luteinizing hormone (LH) secreted from the anterior pituitary. It is well known that LH is able to generate second messengers by adenylate cyclase and the cycle of inositolphospholipids. Also Ca2+ ions are taken into account as an important modulators of Leydig cell steroidogenesis. The aim of this study was to show the effect of macrophage-conditioned medium on the basal and LH stimulated testosterone secretion by mouse Leydig cells in vitro. A source of Leydig cells was the testis of mature, Swiss strain mice. Leydig cells were isolated and cultured for 48 hrs. Testosterone levels were measured radioimmunologically. Intracellular free Ca2+ ions were analysed by computer automatic system 'MAGICAL'. Results indicated that not only LH but also macrophage-conditioned medium were able to modulate testosterone secretion by Leydig cells and increase intracellular calcium concentration. Therefore, it seems possible that the action of proteins secreted from macrophages is mediated through Ca2+ ions which are involved in another signal transduction pathway leading to stimulation of testosterone biosynthesis by Leydig cells in vitro.

Animals↗
PubMed1998

[Studies on DNA content in sperm of men with oligozoospermia].

The aim of our study was to analysis the ploidy of spermatozoa from oligozoospermic individuals. The study was performed in 48 patients with oligozoospermia. Spermatozoal DNA was analysed using MagiCal imaging cytometry. The percentage of aneuploid cells was determined using CYT-2 program. The results indicated aneuploidy in 73% of the examined patients. The amount of cells with abnormal DNA content ranged from 30 to 100%. Monitoring of the DNA in human spermatozoa may have practical applications in establishing the reasons for infertility as well as for screening of the semen for assisted reproduction techniques.

Aneuploidy↗
PubMed1997

Examination of DNA-ploidy in melanocytic nevi cells using video-imaging cytometry.

DNA content in 61 different types of melanocytic nevi was determined by video-imaging cytometry. The nevi were selected for this study because of melanoma risk associated with each nevus, which is 20-50% according to different authors. DNA ploidy was detected in paraffin embedded and fresh tissue material of each patient and the results were comparable. The sample preparation process and video-imaging method were presented in this study, 47 (77.0%) lesions exhibited diploid cell populations, 13 (21.3%) aneuploid and 1 (1.7%) tetraploid cell population. A significant correlation was observed between DNA ploidy measured in fresh tissue and retrospective material. The results indicate the presence of abnormal DNA content in some of the lesions.

DNA, Neoplasm↗
PubMed1997

DNA ploidy in malignant melanoma, skin cancer and pigmented nevi.

The determination of DNA content in human cancers is the subject of increasing interest, particularly in view of its potential clinical applications. There are relatively few studies which describe DNA content of skin neoplasms and pigmented nevi. These studies have shown conflicting results. In the present investigation the authors measured DNA ploidy using flow and video-imaging cytometry in 51 malignant melanomas, 20 skin cancers and 48 pigmented nevi. For DNA measurement paraffin embedded tissues and fresh cell smears were used. Clinical and histological data of malignant melanomas were recorded and correlated with DNA ploidy. DNA histograms were examined for DNA aneuploidy by DNA Index. DNA ploidy in primary lesions of melanomas and their metastases were compared. The aneuploidy rate, found in our observation, was significantly higher in whole malignant melanoma group, in clinical Stage II and III, in tumors with thickness greater then 1.5 mm, tumors with Clark level III, IV and V. Another clinical and histological factors did not show significant correlation with ploidy. Aneuploidy was found in 8 of 20 (45.0%) skin cancers. In the whole population of pigmented nevi aneuploid DNA content was identified in 10 nevi (20.1%). The results of this study suggest that aneuploidy seems to be connected with advanced stage of malignant melanoma but it does not replace other prognostic factors. Both cytometric methods can be used for routine DNA ploidy analysis. Ploidy studies are not useful for predicting metastatic potential of primary melanoma. Results obtained from fresh cell smears and paraffin embedded tissues were identical.

Aneuploidy↗
PubMed1996

Analysis of spermatozoa movement using a video imaging technique.

The aim of this paper is to present methods for a fully computerised analysis of spermatocyte movement. The technique were designed for processing pure images of spermatozoa captured from a light microscope. The techniques described allowed for the reduction of background light inhomogeneity and for the correct detection of moving cells and involved densitometric equalization of background inhomogeneity and implementation of the dynamic threshold, adopted for the recognition of objects. The method for relating cells (found in each frame processed) to movement trajectories used heuristic rules, describing the behaviour of moving cells. This permitted samples containing high numbers of spermatocytes within the observed area to be processed with maximum accuracy.

Humans↗
PubMed1996

Effects of substance P and its antagonist spantide on corticosterone secretion and cytosolic free calcium concentration of dispersed zona fasciculata-reticularis cells of the rat adrenal cortex.

Substance P (SP) did not change basal corticosterone (B) secretion of dispersed zona fasciculata-reticularis cells of the rat adrenal cortex. Conversely, spantide II (SPA), an antagonist of SP receptors, at a concentration 10(-7)/10(-6) M markedly raised it, and the effect was annulled by equimolar concentrations of SP. Both SP and SPA (10(-6) M) increased cytosolic free calcium concentration in our cell preparations; however, the response to SP was immediate, while that to SPA showed a lag-period of 4-5 min. SP concentration-dependently (from 10(-8) M to 10(-5) M) partially inhibited maximally ACTH (10(-8) M)-induced stimulation of B secretion of dispersed cells, and unexpectedly a similar effect was observed after SPA exposure. In light of these findings, the conclusion is drawn that SP, under basal conditions, does not exert a direct modulatory action of B secretion of rat adrenocortical cells. However, the possibility remains to be explored that SP may play a role in quenching, via a receptor-independent mechanism, the exceedingly high glucocorticoid responses to ACTH of rat adrenocortical cells.

Adrenocorticotropic Hormone↗
PubMed1995

Effects of neurokinin-A on the rat hypothalamo-pituitary-adrenal axis.

The effects of neurokinin-A (NKA) on the rat hypothalamo-pituitary-adrenal (HPA) axis were studied in vivo and in vitro. A subcutaneous injection of 1 or 3 nmol/100 g NKA did not alter plasma ACTH concentration. The lower dose of NKA evoked a transient rise in plasma corticosterone (B) concentration (PBC) at 30 min, and did not change plasma aldosterone (ALDO) concentration (PAC). The higher dose of NKA increased PBC at 30 and 60 min, and PAC at 30, 60 and 120 min. NKA did not affect basal ALDO secretion of dispersed zona glomerulosa (ZG) cells, but it markedly enhanced basal B production by dispersed zona fasciculata/reticularis (ZF/R) cells (minimal and maximal effective concentrations were 10(-9) M and 10(-6) M). Video-imaging analysis showed that 10(-6) M NKA increased intracellular Ca2+ concentration in dispersed ZF/R cells, but not in ZG ones. These findings indicate that NKA exerts a stimulatory action on the rat adrenal secretory activity, which is independent of any effect on the pituitary ACTH release: the B secretagogue action seems to be due to a direct effect of NKA on ZF/R cells, while the ALDO secretagogue action is not direct. but probably mediated by factor(s) other than ACTH.

Adrenocorticotropic Hormone↗