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Biomedical subjects

K Ferrara

Publications and source records attributed to K Ferrara.

7 recordsLinked to original sources
PubMed2001

Threshold of fragmentation for ultrasonic contrast agents.

Ultrasound contrast agents are small microbubbles that can be readily destroyed with sufficient acoustic pressure, typically, at a frequency in the low megaHertz range. Microvascular flow rate may be estimated by destroying the contrast agent in a vascular bed, and estimating the rate of flow of contrast agents back into the vascular bed. Characterization of contrast agent destruction provides important information for the design of this technique. In this paper, high-speed optical observation of an ultrasound contrast agent during acoustic insonation is performed. The resting diameter is shown to be a significant parameter in the prediction of microbubble destruction, with smaller diameters typically correlated with destruction. Pressure, center frequency, and transmission phase are each shown to have a significant effect on the fragmentation threshold. A linear prediction for the fragmentation threshold as a function of pressure, when normalized by the resting diameter, has a rate of change of 300 kPa/microm for the range of pressures from 310 to 1200 kPa, and a two-cycle excitation pulse with a center frequency of 2.25 MHz. A linear prediction for the fragmentation threshold as a function of frequency, when normalized by the resting diameter, has a rate of change of -1.2 MHz/microm for a transmission pressure of 800 kPa, and a two-cycle excitation pulse with a range of frequencies from 1 to 5 MHz.

Contrast Media↗
PubMed2000

Noninvasive imaging of inflammation by ultrasound detection of phagocytosed microbubbles.

BACKGROUND: We have previously shown that microbubbles adhere to leukocytes in regions of inflammation. We hypothesized that these microbubbles are phagocytosed by neutrophils and monocytes and remain acoustically active, permitting their detection in inflamed tissue. METHODS AND RESULTS: In vitro studies were performed in which activated leukocytes were incubated with albumin or lipid microbubbles and observed under microscopy. Microbubbles attached to the surface of activated neutrophils and monocytes, were phagocytosed, and remained intact for up to 30 minutes. The rate of destruction of the phagocytosed microbubbles on exposure to ultrasound was less (P</=0.05) than that of free microbubbles at all acoustic pressures applied. Intravital microscopy and simultaneous ultrasound imaging of the cremaster muscle was performed in 6 mice to determine whether phagocytosed microbubbles could be detected in vivo. Fifteen minutes after intravenous injection of fluorescein-labeled microbubbles, when the blood-pool concentration was negligible, the number of phagocytosed/attached microbubbles within venules was 7-fold greater in tumor necrosis factor-alpha (TNF-alpha)-treated animals than in control animals (P<0.01). This increase in retained microbubbles resulted in a 5- to 6-fold-greater (P<0.01) degree of ultrasound contrast enhancement than in controls. CONCLUSIONS: After attaching to activated neutrophils and monocytes, microbubbles are phagocytosed intact. Despite viscoelastic damping, phagocytosed microbubbles remain responsive to ultrasound and can be detected by ultrasound in vivo after clearance of freely circulating microbubbles from the blood pool. Thus, contrast ultrasound has potential for imaging sites of inflammation.

Animals↗
PubMed1999

Acoustic radiation force in vivo: a mechanism to assist targeting of microbubbles.

The goal of targeted imaging is to produce an enhanced view of physiological processes or pathological tissue components. Contrast agents may improve the specificity of imaging modalities through selective targeting, and this may be particularly significant when using ultrasound (US) to image inflammatory processes or thrombi. One means of selective targeting involves the attachment of contrast agents to the desired site with the use of a specific binding mechanism. Because molecular binding mechanisms are effective over distances on the order of nanometers, targeting effectiveness would be greatly increased if the agent is initially concentrated in a particular region, and if the velocity of the agent is decreased as it passes the potential binding site. Ultrasonic transmission produces a primary radiation force that can manipulate microbubbles with each acoustic pulse. Observations demonstrate that primary radiation force can displace US contrast agents from the center of the streamline to the wall of a 200-microm cellulose vessel in vitro. Here, the effects of radiation force on contrast agents in vivo are presented for the first time. Experimental results demonstrate that radiation force can displace a contrast agent to the wall of a 50-microm blood vessel in the mouse cremaster muscle, can significantly reduce the velocity of flowing contrast agents, and can produce a reversible aggregation. Acoustic radiation force presents a means to localize and concentrate contrast agents near a vessel wall, which may assist the delivery of targeted agents.

Acoustics↗
PubMed1997

Color flow mapping.

Color flow mapping systems have become widely used in the short time since their development. These systems overlay a pseudo-color velocity map upon the gray-scale two-dimensional image. Between 4 and 16 pulses are directed to each line-of-sight, and this requirement reduces the frame rate in comparison with the gray-scale image. Other limitations of color flow mapping include its ability to estimate only the velocity toward or away from the transducer and an increase in the variance in comparison with spectral Doppler. Potential artifacts include aliased velocities and the detection of flow in hypoechoic or hyperechoic nonvascular structures. Clinical applications include cardiology, studies of the abdominal and peripheral vasculature, evaluation of organ perfusion and the differentiation of tumors. Most current systems use narrowband estimators that examine a fixed sample volume and detect a change in phase between two pulses. Wideband estimators that can track red blood cells in two or three dimensions are under evaluation. Narrowband estimators, including the autocorrelator, the short Fourier transform and second order autoregressive filters, are compared with wideband estimators including cross-correlation, sum-absolute-difference and the wideband maximum likelihood estimator. Because the intensity of blood echoes is far smaller than echoes from surrounding tissue, high pass filters have been developed that can reject the larger signal from tissue using the return from a small number of pulses. Other areas of research include strategies for flow estimation with contrast agents, three-dimensional color flow mapping and power Doppler flow mapping.

Echocardiography, Doppler, Color↗
PubMed1997

An assessment of ergonomic educational resources. An important tool for occupational health nurses.

1. Extensive ergonomic education and training resources exist at the organizations responding to OSHA's survey. However, the availability and use of ergonomic resources by employees is low and varies considerably among industry types. 2. Limited research is available about effective methods of ergonomic training to workers and their supervisors. A critical need exists to determine the best methods for the successful dissemination of ergonomic information. 3. Occupational health nurses can contribute significantly to the advancement of ergonomic knowledge and skills, and the dissemination of ergonomic information in the workplace, primarily through health education and research.

Education, Nursing, Continuing↗