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Biomedical subjects

K Eto

Publications and source records attributed to K Eto.

At least 163 records · Page 9Linked to original sources
PubMed1989

[Blue-rubber-bleb-nevus syndrome presented vascular dementia and chronic DIC--a case report].

We reported a female with vascular dementia due to chronic disseminated intravascular coagulation (DIC). The patient underwent operation for skin hemangiomas three times at age 18, 27, and 38. At age 43, she presented a slowly progressive dementia. She had bluish, compressive hemangiomas of skin and upper gastrointestinal tract, and she was suspected to have hemangiomas of brain, pleura, mediastinum, liver and pancreas. The hemangiomas were diagnosed blue-rubber-bleb-nevus syndrome (BRBNS). Laboratory data revealed persistent increase of serum FDP, FDP-D, FDP-E, and D-dimer. This condition was considered chronic DIC, therefore this case was the first case of combination of BRBNS and Kasabach-Merritt syndrome (chronic DIC). Brain MRI demonstrated bilateral lesions of paraventricular deep white matter and hemangioma of left temporal lobe. Because cerebral CT showed no contrast enhancement in paraventricular lesions, the lesions were considered multiple cerebral infarctions. Central nervous system (CNS)involvement in BRBNS is rare, and most cases of CNS involvement were associated with CNS angiomata. In this case it was suggested that brain was the target organ of DIC because thrombomodulin is absent or few in the human brain, therefore vascular dementia (multiple cerebral infarctions) developed due to chronic DIC.

Adult↗
PubMed1989

Different histochemical findings in the brain produced by mercuric chloride and methyl mercury chloride in rats.

The chemical form of mercury reactive by the histochemical technique was studied by using rats and mice treated with mercuric chloride (HgCl2) and methyl mercury chloride (MeHgCl). Mercury granules were demonstrated histochemically in the brain of HgCl2-treated rats with higher levels of inorganic mercury. However, mercury granules were not demonstrated in the brain of MeHgCl-treated rats in spite of a considerably high organic mercury level. In rats and mice with significant biotransformation of injected MeHg to inorganic mercury, the appearance of the peak inorganic mercury level and mercury granules in the brain seemed to occur on the same day. These results suggested that mercury granules in the brain represent inorganic mercury, but not organic mercury. In the brains showing mercury granules, the lowest level of inorganic mercury was 0.12 micrograms/g in HgCl2-treated rats, 0.14 micrograms/g in MeHgCl-treated rats, and 0.12 micrograms/g in MeHgCl-treated mice. These values were similar to the level of inorganic mercury in the brain from human autopsy cases. Mercury granules were demonstrated histochemically in nerve cells, choroid plexus and phagocytes in the brain of both HgCl2-and MeHgCl-treated rats, and in ependyma of MeHgCl-treated rats. The level of inorganic mercury of the brains showing mercury granules in phagocytes was lower in MeHgCl-treated rats than in HgCl2-treated rats. The numbers of mercury granules in nerve cells increased with the rising level of inorganic mercury in the brain of MeHgCl-treated rats.

Animals↗
PubMed1988

An autosomal dominant mutation of facial development in a transgenic mouse.

We have created a transgenic mouse which showed an autosomal dominant mutation of facial development. This facial malformation was characterized by a short snout and a twisted upper jaw. All offspring showing the dysmorphic phenotype carried the injected gene. In order to analyze the primary cause of this mutation, newborn mice and embryos were examined. The outcome was that the malformation of nasal and premaxillary bone was not the primary defect but was a secondary event. The primary cause of this dysmorphism was a developmental defect in the first branchial arch. Genomic DNA fragments flanking the insertion site of this mutant mouse were cloned. Using these fragments, we have assigned the integration site to chromosome 13. The gene responsible for a previously reported mutant mouse, one which also has a short snout, is also reported to be on chromosome 13. In the fragments flanking the insertion site of the transgenic mouse, at least one fragment was highly conserved in mammals. These results indicate that this malformation is due to the insertional disruption of a host gene. However, the possibility that this mutation is caused by an inappropriate expression of the injected gene still remains to be investigated.

Animals↗
PubMed1988

Establishment and characterization of a new human renal cell carcinoma cell line (KRC/Y).

A new renal cell carcinoma (RCC) cell line (KRC/Y) has been established from a surgical specimen of a 41-yr-old Japanese female patient with RCC composed of both clear cells and granular cells. This cell line has been maintained for more than 15 mo. through 45 passages with a stable growth. KRC/Y cells have clear or eosinophilic polygonal cytoplasm and round to oval nuclei with one or two nucleoli, and proliferate in a pavementlike cell arrangement with a lack of contact inhibition. By electron microscopy, these cells contain abundant fat droplets and glycogen granules or well-developed organelles or both, which were also observed in the original tumor. The doubling time of these cells at the 15th passage was 73 h. The chromosome number was from 37 to 45 with a hypodiploid modal number of 42. Tumorigenicity was identified by tumor formation after subcutaneous injections of KRC/Y cells in nude mice, which showed close resemblance to the original tumor by light and electron microscope observations.

Adult↗
PubMed1986

Revascularization of reversed dermis grafts.

The process of vascularization and the vascular patterns in reversed dermis grafts (RDG) and full-thickness skin grafts (FTSG) in the rat were studied at various intervals after transplantation by a combination of microangiography and histologic techniques. In the early stages the RDG became vascularized more rapidly than the FTSG. However, the inflammatory reaction in the RDG persisted for longer periods than that in the FTSG in the later stages. The prolonged inflammatory reaction in the RDG was presumed to be due to the lack of epidermis for two to three weeks after surgery. Comparatively severe contraction of RDG may result from this prolonged inflammation and the delayed epithelialization.

Animals↗
PubMed1986

In vitro release of tegafur from a fatty-base suppository and in vivo bioavailability of tegafur.

This study was designed to determine the in vitro release of tegafur from a suppository and the in vivo bioavailability of tegafur in rats. Two different suppository preparations (product A-1 and product A-2) containing 750 mg of tegafur were tested for in vitro release of tegafur by the Muranishi Method (membrane diffusion method) and the partially modified paddle method (permeability through dialysis tubing). When determined by either method, the amount of tegafur released from product A-2 during the whole experimental period was significantly greater than that released from product A-1. When tested by the Muranishi method, however, the difference in the amount released during the first 10-min period was not significant. A greater bioavailability of tegafur after rectal administration was obtained by product A-2 more than product A-1. A significant correlation was observed between the in vitro release and the in vivo bioavailability. The present results indicate that there are considerable differences in physiochemical characteristics between product A-1 and product A-2.

Animals↗
PubMed1986

Effects of aggregation on methamphetamine toxicity in mice.

Methamphetamine (MA) toxicity in aggregated mice was studied by varying the number of mice and the proportion of MA treated mice kept in the same confined space. The lethality was measured 24 h after intraperitoneal injections of MA at doses ranging from 10 to 100 mg/kg. MA lethality, over a wide dose range (15 to 50 mg/kg), was higher in aggregated mice than in those maintained in isolation. The greater the proportion of MA-treated mice in aggregation was, the higher the MA lethality was. In aggregations of 10 mice, MA was lethal at lower doses than in aggregations of 5 mice. These results indicate that the lethality of MA is influenced by confinement and aggregation.

Animals↗
PubMed1986

Stage specific response of the mesenchyme to excess vitamin A in developing rat facial processes.

The effects of excess retinol (vitamin A alcohol) on facial process formation were examined in cultured rat embryos. The embryos were explanted at day 11 of gestation (plug day = 0) and cultured for up to the 50-somite stage in rat serum containing added 1 microgram/ml or 10 micrograms/ml retinol. The reduction in outgrowth of facial processes was observed in 1-microgram/ml-retinol-treated embryos and this type of malformation was found to be more severe in 10-micrograms/ml-retinol-treated embryos. Histological findings of 10-micrograms/ml-retinol-treated embryos at the 50-somite stage showed that the nasal epithelium was developed but folded. In the mesenchyme, there were necrotic cells. Thymidine incorporation by mesenchymal cells of facial processes was determined. At the 50-somite stage, the uptake was decreased to 66.4% of control value at 1 microgram/ml retinol, whereas the addition of the same dose of retinol did not cause the inhibition at the 36-, 40-, and 42-somite stages. The uptake at the 50-somite stage was decreased to 23.0% as a result of the 10 micrograms/ml retinol treatment. Furthermore, the effects of cartilage-derived factor (CDF) on the facial mesenchyme were examined; 20 micrograms/ml of CDF stimulated the 3H-thymidine incorporation in facial mesenchyme, especially after the 42-somite stage. By the addition of 10 micrograms/ml retinol, the incorporation decreased to 45.7% at the 38-somite stage, but it did not decline with concomitant use of CDF after the 38-somite stage.(ABSTRACT TRUNCATED AT 250 WORDS)

Abnormalities, Drug-Induced↗
PubMed1986

Relative contributions of the facial processes to facial development: a microsurgical assay.

The facial processes, which consist of the medial nasal process (MNP), the lateral nasal process (LNP), and the maxillary process (MP), are basal components in facial morphogenesis, especially upper lip formation. To examine the relative role of each facial process in normal or abnormal facial development, rat embryos that had had a part of each facial process excised were cultured for 72 hr in vitro from gestational day 11.5 (plug day = day 0). At the termination of culture, although the epithelial wound was healed over, the defect was observed corresponding to excised region in form. Only in the MNP-excised group was cleft liplike malformation observed, but in other groups this malformation was absent or at a lower rate. This suggests that the medial nasal process in this stage plays a critical role in normal facial development as well as cleft lip formation.

Animals↗
PubMed1986

Appearance of a unique cell type in the fusion sites of facial processes.

The contact sites between the medial and lateral nasal processes during the period of facial formation of the mouse embryo were examined by light and electron microscopy. Characteristic superficial cells were observed at the transitional regions between the surface ectoderms and the nasal epithelia at the end of the isthmus, where the initial contact of the opposing nasal processes took place. At the later stage the contact sites extended to the bottom of the ravine formed by the two nasal processes, where the superficial cells always seemed to bridge the area between the nasal processes. These superficial cells had a large, clear nucleus and abundant cytoplasm as well as the common structural features characteristic of the embryonic cells. These cells were also observed on the surface near the contact site in the presumptive fusion area. These observations suggest that these superficial cells play a critical role in the epithelial adhesion of the medial and lateral nasal processes throughout the fusion.

Animals↗