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Biomedical subjects

K Dornbusch

Publications and source records attributed to K Dornbusch.

At least 91 records · Page 5Linked to original sources
PubMed1976

Antibacterial susceptibility testing by the dip-slide technique: a methodological evaluation.

Antibacterial susceptibility testing of urine specimens by the dip-slide method was compared to the standardized disc diffusion method. A boarder line of 12 mm inhibition zone on the slide could be used to select strains resistant to sulphisadimidine, ampicillin, nitrofurantoin or nalidixic acid. In the case of the sulphonamide, the results were grewtly influenced by composition of the medium. Susceptibility testing with the dip-slide method seems to be a useful test to differentiate strains likely to respond to therapy from resistant strains.

Ampicillin↗
PubMed1976

Concentrations of oxytetracycline, tetracycline and doxycycline in mandibular osteitis.

Treatment of osteitis in the mandible after surgery is still a clinical problem. Levels of three tetracyclines--doxycycline, oxytetracycline and tetracycline--were measured in serum and dental alveolar serum in 30 patients undergoing oral surgery. The serum concentrations were higher than the dental alveolar serum concentrations in all patients. The maximal concentration in the alveolar serum for doxycycline was between 3.0 and 3.5 mug/ml while the corresponding values for oxytetracycline and tetracycline were between 1.0 and 2.0 mug/ml. When the dental alveolar serum concentrations of the various tetracycline analogues were related to their range of inhibitory concentrations for microorganisms isolated from mandibular osteitis, it was found that each drug reached levels sufficient to inhibit most but not all strains.

Administration, Oral↗
PubMed1976

Doxycycline concentration levels in bone, soft tissue and serum after intravenous infusion of doxycycline. A clinical study.

As part of a longitudinal study, the influence of preoperative single dose doxycycline treatment on the overall incidence of early postoperative wound infections in orthopaedic surgery, 34 patients with various fractures were given an intravenous infusion of doxycycline 2-4 hours before the operation. The serum concentrations of doxycycline were determined on specimens taken during the operation and on 4 other occasions, the last being 48 hours after operation. All patients except 4 had serum concentration levels of 0.4 mug per ml or above at 48 hours. During the operation, specimens of skin, subcutaneous tissue, muscle and bone were also taken. The doxycycline concentrations were determined in the various tissues and correlated to the concomitant serum concentration. The results were analysed with regard to the possible influence of preoperative single dose doxycycline treatment for prevention of early postoperative infections.

Adipose Tissue↗
PubMed1976

The detection of doxycycline activity in human bone.

Measurements of antibacterial activity in bone from 25 patients undergoing orthopaedic surgery were performed after preoperative treatment with doxycycline in a single dose of 200 mg. The antibiotic was given by intravenous infusion during 30-40 minutes, and the operation was performed 2-6 hours after administration of the dose. Using the agar diffusion method, the concentrations ranged from 0.05-0.6 mug/g. Using the electrophoretic method, higher levels of doxycycline were found, possibly as a consequence of a diminution in the binding capacity of the bone.

Adolescent↗
PubMed1975

Extracellular proteins in five clostridial species from human infections.

347 strains from human infections were identified by gas-liquid chromatography of metabolic products and by conventional tests. Simple agar-plate assays were used to analyze the ability to form extracellular proteins. More than 90% of all strains were hemolytic on agar containing rabbit erythrocytes and all were gelatinase producers. All strains of C. bifermantans, C. sordelli, and C. sporogenes were also caseinolytic on skimmed-milk agar, but strains of C. perfringens, C. novyi types A and B were not. Less than 10% of C. perfringens strains were producers of elastase and staphylolytic enzyme and all other species were non-producers. All C. perfringens, C. novyii, C. bifermentans, and C. sordelli were lecithinase producers, but C. sporogenes was not. All strains of C. sporogenes formed deoxyribonuclease, while a varying number of the other species showed a positive reaction.

Bacterial Proteins↗
PubMed1975

Antibiotic susceptibility testing of anaerobic bacteria by the standardized disc diffusion method with special reference to bacteroides fragilis.

The susceptibility of 90 strains of Bacteroides fragilis to 16 antibacterial drugs was determined by the agar plate dilution test and the disc diffusion test. Good correlation of results was obtained with the two methods, and regression line analysis could be performed for beta-lactam antibiotics, erythromycin, tetracyclines, lincomycin and clindamycin. Thus the standardized disc diffusion method can be used for susceptibility testing, but only a clear distinction between sensitive and resistant strains can be seen.

Agar↗
PubMed1975

Antibiotic susceptibility of Clostridium species isolated from human infections.

In antibacterial susceptibility testing by the agar plate dilution method with 252 strains of Clostridium perfringens, 22 strains of C. novyi type A and 7 of C. novyi type B, with 21 strains of C. bifermentans, 12 strains of C. sordellii and 18 of C. sporogenes, the majority of the strains were inhibited by penicillin G, cephalothin, chloramphenicol, erythromycin, tinidazole and metronidazole. Resistance to lincomycin and clindamycin was noticed among strains of all clostridia species as well as to tetracycline and doxycycline among strains of C. perfringens.

Anti-Bacterial Agents↗
PubMed1975

Regression line analysis for five antibiotics with strains of Clostridium species.

The susceptibility of 90 strains of clostridia to 5 antibacterials was determined by the agar plate dilution method and the disc diffusion test. The correlations between minimum inhibitory concentration and inhibition zone were quite acceptable for lincomycin, clindamycin, tetracycline and doxycycline to calculate regression lines by the least square method. The strains showed comparatively wide ranges of susceptibility. For erythromycin, no regression line could be calculated, since all the strains tested were susceptible over a narrow range of concentrations.

Anti-Bacterial Agents↗