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Biomedical subjects

K Dholakia

Publications and source records attributed to K Dholakia.

12 recordsLinked to original sources
PubMed2006

Observation of bistability and hysteresis in optical binding of two dielectric spheres.

Using a dual-beam fiber optic trap, we have experimentally observed bistability and hysteresis in the equilibrium separations of a pair of optically bound dielectric spheres in one dimension. These observations are in agreement with our coupled system model in which the dielectric spheres modify the field propagation, and the field self-consistently determines the optical forces on the spheres. Our results reveal hitherto unsuspected complexity in the coupled light-sphere system.

Journal Article↗
PubMed2004

Optically bound microscopic particles in one dimension.

Counterpropagating light fields have the ability to create self-organized one-dimensional optically bound arrays of microscopic particles, where the light fields adapt to the particle locations and vice versa. We develop a theoretical model to describe this situation and show good agreement with recent experimental data [Phys. Rev. Lett. 89, 128301 (2002)] for two and three particles, if the scattering force is assumed to dominate the axial trapping of the particles. The extension of these ideas to two- and three-dimensional optically bound states is also discussed.

Journal Article↗
PubMed2003

Microfluidic sorting in an optical lattice.

The response of a microscopic dielectric object to an applied light field can profoundly affect its kinetic motion. A classic example of this is an optical trap, which can hold a particle in a tightly focused light beam. Optical fields can also be used to arrange, guide or deflect particles in appropriate light-field geometries. Here we demonstrate an optical sorter for microscopic particles that exploits the interaction of particles-biological or otherwise-with an extended, interlinked, dynamically reconfigurable, three-dimensional optical lattice. The strength of this interaction with the lattice sites depends on the optical polarizability of the particles, giving tunable selection criteria. We demonstrate both sorting by size (of protein microcapsule drug delivery agents) and sorting by refractive index (of other colloidal particle streams). The sorting efficiency of this method approaches 100%, with values of 96% or more observed even for concentrated solutions with throughputs exceeding those reported for fluorescence-activated cell sorting. This powerful, non-invasive technique is suited to sorting and fractionation within integrated ('lab-on-a-chip') microfluidic systems, and can be applied in colloidal, molecular and biological research.

Capsules↗
PubMed2003

Observation of the transfer of the local angular momentum density of a multiringed light beam to an optically trapped particle.

We observe the spinning and orbital motion of a microscopic particle trapped within a multiringed light beam that arises from the transfer of the spin and orbital components of the light's angular momentum. The two rotation rates are measured as a function of the distance between the particle and the axis of the trapping beam. The radial dependence of these observations is found to be in close agreement with the accepted theory.

Journal Article↗
PubMed2003

Interfering Bessel beams for optical micromanipulation.

We examine the properties of interfering high-order Bessel beams. We implement an experimental setup that allows us to realize these interferograms, using interfering Laguerre-Gaussian beams and an axicon. We demonstrate the use of such beams for controlled rotation of microscopic particles in optical tweezers and rotators. The self-healing properties of interfering Bessel beams allow the simultaneous manipulation and rotation of particles in spatially separated sample cells.

Journal Article↗
PubMed2002

One-dimensional optically bound arrays of microscopic particles.

A one-dimensional optically coupled array of colloidal particles is created in a potential well formed by two counterpropagating Gaussian light beams. This array has analogies to linear chains of trapped atomic ions. Breathing modes and oscillations of the center of mass are observed. The stability of the array is in accordance with the Kramers model.

Journal Article↗
PubMed2002

Simultaneous micromanipulation in multiple planes using a self-reconstructing light beam.

Optical tweezers are commonly used for manipulating microscopic particles, with applications in cell manipulation, colloid research, manipulation of micromachines and studies of the properties of light beams. Such tweezers work by the transfer of momentum from a tightly focused laser to the particle, which refracts and scatters the light and distorts the profile of the beam. The forces produced by this process cause the particle to be trapped near the beam focus. Conventional tweezers use gaussian light beams, which cannot trap particles in multiple locations more than a few micrometres apart in the axial direction, because of beam distortion by the particle and subsequent strong divergence from the focal plane. Bessel beams, however, do not diverge and, furthermore, if part of the beam is obstructed or distorted the beam reconstructs itself after a characteristic propagation distance. Here we show how this reconstructive property may be utilized within optical tweezers to trap particles in multiple, spatially separated sample cells with a single beam. Owing to the diffractionless nature of the Bessel beam, secondary trapped particles can reside in a second sample cell far removed ( approximately 3 mm) from the first cell. Such tweezers could be used for the simultaneous study of identically prepared ensembles of colloids and biological matter, and potentially offer enhanced control of 'lab-on-a-chip' and optically driven microstructures.

Journal Article↗
PubMed2002

Creation and manipulation of three-dimensional optically trapped structures.

An interferometric pattern between two annular laser beams is used to construct three-dimensional (3D) trapped structures within an optical tweezers setup. In addition to being fully translatable in three dimensions, the trapped structure can be rotated controllably and continuously by introducing a frequency difference between the two laser beams. These interference patterns could play an important role in the creation of extended 3D crystalline structures.

Journal Article↗
PubMed2001

Controlled rotation of optically trapped microscopic particles.

We demonstrate controlled rotation of optically trapped objects in a spiral interference pattern. This pattern is generated by interfering an annular shaped laser beam with a reference beam. Objects are trapped in the spiral arms of the pattern. Changing the optical path length causes this pattern, and thus the trapped objects, to rotate. Structures of silica microspheres, microscopic glass rods, and chromosomes are set into rotation at rates in excess of 5 hertz. This technique does not depend on intrinsic properties of the trapped particle and thus offers important applications in optical and biological micromachines.

Animals↗
PubMedDate not supplied

Cell cytometry with a light touch: sorting microscopic matter with an optical lattice.

Lab-on-a-chip design is a key technology for increasing both the reliability and the functionality of many different preparation and diagnostic techniques in biomedicine. The drive towards ever more integrated lab-on-a-chip designs requires increasingly complex microfluidic systems. In order to build these systems, non-invasive actuators such as pumps, filters and mixers are required. We have demonstrated microfluidic sorting based on a 3D interference pattern, formed from multiple coherent laser beams, which has the potential to fulfil all the above criteria. By interfering five laser beams from a fibre laser at 1070 nm, we have formed a 3D optical lattice. When particles flow through the optical lattice their trajectories depend upon the force exerted on the particle by the optical lattice, in combination with the drag force exerted by the fluid flow. Hence, with the strength of a particle's interaction with the lattice determining the total force exerted upon it, its trajectory is determined by its physical properties. These properties include refractive index, size and shape, giving a range of criteria with which to sort an analyte. We have shown separation at 45 degrees of polymer from silica microspheres (by refractive index), the separation of protein microcapsules and the sorting of erythrocytes from lymphocytes. The interference pattern can be tailored to the particles and if a blockage occurs, the laser can simply be switched off, unlike solid-state micro-sorters, so that no jamming occurs. Efficiencies in excess of 95% have been achieved.

Animals↗