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Biomedical subjects

K Busch

Publications and source records attributed to K Busch.

At least 37 records · Page 2Linked to original sources
PubMed1996

Restricted geographical extension of the association of a glucagon receptor gene mutation (Gly40Ser) with non-insulin-dependent diabetes mellitus.

A mutation within the glucagon receptor gene (GRG) at codon 40 (Gly40Ser) was reported to be associated with non-insulin-dependent diabetes mellitus (NIDDM) in France and Sardinia. Since the frequency of the mutation, about 5% in the French and 8% in the Sardinian group was higher than that of any of the candidate gene mutations described so far, it appeared to be relevant to determine the prevalence within different populations. While a single mutation has not been found recently either in a Japanese, a Finnish or a Dutch cohort, but only in a British study in 2.2% of NIDDM patients, in this study we investigated the association of this mutation with NIDDM in 104 German patients by polymerase chain reaction-restriction fragment length polymorphism analysis (PCR-RFLP). None of the German NIDDM patients had a Gly40Ser mutation. The results indicate that the mutation plays little role in susceptibility to NIDDM in this German region, and also indicate the genetic heterogeneity in NIDDM, and further emphasize the importance of studies in different ethnic groups.

Aged↗
PubMed1996

Corrections to the target and critical values for the National Institute for Occupational Safety and Health validation tests.

In 1974 the National Institute for Occupational Safety and Health (NIOSH) and the Occupational Safety and Health Administration joined to complete exposure standards promulgated by federal regulations. In that effort NIOSH scientists developed an accuracy criterion (AC) and a statistical protocol for evaluating its fulfillment. That AC and those procedures have been widely used ever since. This article presents corrections to the target and critical coefficients of variation published as part of the statistical protocol.

Bias↗
PubMed1996

Mycoplasmas associated with bovine conjunctivitis and keratoconjunctivitis.

In two separate herds of fattening calves a sudden-onset outbreak of ocular disease with profuse lacrimation occurred. The disease resembled the early stage of infectious bovine keratoconjunctivitis but after a few days the clinical signs of bronchopneumonia appeared. From conjunctival swabs Mycoplasma (M.) bovigenitalium, M. bovirhinis and infectious bovine rhinotracheitis (IBR) virus were isolated. Moraxella bovis infection was not established. In one of the herds M. bovigenitalium was also found in the pneumonic lungs of dead calves. In one herd M. bovoculi was isolated from a cow with chronic keratoconjunctivitis, housed together with affected calves. Mycoplasmas were not isolated from ocular swabs of six bulls originating from a Reproductive Centre with temporary occurrence of unilateral serous conjunctivitis resistant to antibiotic therapy.

Animals↗
PubMed1995

Identity elements of human tRNA(Leu): structural requirements for converting human tRNA(Ser) into a leucine acceptor in vitro.

We have previously shown that the exchange of the discriminator base A73 of human tRNA(Leu) for G is alone sufficient to achieve complete loss of leucine acceptance and to create an efficient serine acceptor. The reverse identity switch, however, which was studied using T7 RNA polymerase transcripts of in vitro mutagenized tRNA genes, reveals a far more complex pattern of identity elements for tRNA(Leu). Introduction of the following tRNA(Leu)-specific structures is necessary to transform human tRNA(Ser) into an efficient leucine acceptor: the discriminator base A73, the base pairs C3:G70, A4:U69 and G5:C68 of the acceptor stem, C20a of the DHU loop and the long extra arm. In contrast to tRNA(Ser), human tRNA(Leu) identity requires both the sequence and the correct orientation of the long extra arm, whereas only its orientation is essential for serine identity.

Acylation↗
PubMed1995

Natural uterine Mycoplasma pulmonis infection in female rats.

Uterine washings from 124 apparently healthy and non-pregnant female Wistar rats of different ages were cultured for mycoplasmas and bacteria. The animals originated from four conventional breeding colonies which were known to be chronically infected with M. pulmonis from the previous microbiological examination. Mycoplasmas were isolated from the uterus in 30.6% of examined females. All the isolates were biochemically and seriologically identified as M. pulmonis. Uterine colonization with this organism was first evidenced in non-mated female rats at the age of three months. After mating the number of infected females rapidly increased. This observation points out the microbiologically uncontrolled mating as an important factor in the distribution of genital infection within the colony. Bacterial examination of uterine washings revealed only ubiquitous organisms in some animals. Gross lesions in the form of purulent salpingitis and mild endometritis were observed only in two animals.

Animals↗
PubMed1989

Developmental and disruptive behavior disorders among delinquents.

Medical histories, psychological tests, psychiatric examinations, and social investigations were reviewed to determine the frequency of independent diagnoses of retardation, attention deficit disorder with hyperactivity (ADD-H), and attention deficit disorder without hyperactivity (ADD). In a sample of 1,956 (384 females and 1,572 males) adjudicated delinquents with a mean age of 13.9 +/- 1.9 years, 9% had ADD-H, 15% were retarded, and 46% had ADD. The reliabilities of the diagnoses were 0.95 for retardation, 0.91 for ADD-H, and 0.94 ADD. Retarded delinquents had the greatest scholastic delays followed by the ADD-H and ADD-adolescent offenders.

Adolescent↗