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Biomedical subjects

K Brune

Publications and source records attributed to K Brune.

At least 271 records · Page 15Linked to original sources
PubMed1974

Assessment of phagocytic and antimicrobial activity of human granulocytes.

A microassay for assessing two functions of polymorphonuclear leukocytes in the presence or absence of complement is presented. Requiring only minute amounts of blood (0.1 ml) and a minimum of laboratory equipment (microscope, incubator, and centrifuge), it allows the quantitation of phagocytosis and intracellular killing of microorganisms by leukocytes. To demonstrate the value of this assay, the phagocytic and microbicidal activity of leukocytes from healthy subjects and patients against Candida albicans was investigated. Apart from individual cases, no differences in the phagocytic activity between groups of healthy subjects and patients with candida vaginitis or different types of cancer could be found. However, the killing capacity of the leukocytes from women with recurrent candida vaginitis was reduced. The leukocytes of one patient showing a very low killing capacity lacked myeloperoxidase. Also, low values of killing were seen with leukocytes from three patients suffering from osteosarcoma, chronic lymphatic leukemia, or Hodgkins disease.

Journal Article↗
PubMed1972

Microbicidal activity of peroxidaseless chicken heterophile leukocytes.

Polymorphonuclear leukocytes (PMN) of the domestic chicken lack myeloperoxidase and, therefore, may be useful for studies of myeloperoxidase-independent antimicrobial mechanisms. Before such studies were undertaken, it was important to investigate the antimicrobial capacity of these cells against species of opportunistic pathogens that cause infection in humans with defective PMN function. In vitro, chicken PMN, like normal human PMN, readily phagocytized and killed Staphylococcus albus. They also killed Serratia marcescens, Escherichia coli, and Candida albicans. Cytochemical methods confirmed the absence of myeloperoxidase from chicken PMN.

Animals↗