Search PubMed⌕ Search

Biomedical subjects

K Berry

Publications and source records attributed to K Berry.

At least 91 records · Page 5Linked to original sources
PubMedDate not supplied

Sucrase and cellular development.

The cellular changes that take place as the intestinal cell migrates from crypt to villus are morphologically and biochemically remarkable. It is fortunate that many of these phenomena can be delineated by following enzymic activities. Sucrase-isomaltase is a particularly fascinating enzyme complex because it is a marker of the differentiated cell. Sucrase is inducible with steroids and protected by the substrate sucrose. Purified enzyme can be used to stimulate production of specific antibodies in goats; these antibodies have been used as probes to locate enzymically active and inactive antigen in the cells of the crypt and villus respectively. Further examination of the enzyme has indicated a molecular weight of 200 000--350 000. These higher molecular weight components are located in the brush border of the enterocytes. Lower molecular weight subunits are antigenically active and are in the cytosol. It is assumed that these smaller components are enzymically inactive pre-combination subunits of the sucrase-isomaltase complex and that the sucrase-isomaltase of the brush border is an aggregate of these subunits. The California sea lion, which is deficient in intestinal sucrase activity, does have isomaltase activity. This finding supports the concept that there are different gene complexes for sucrase and for isomaltase.

Aging↗
PubMedDate not supplied

Root cause analysis in response to a "near miss".

Root cause analysis is a technique used in determining the real cause of an event. The Sentinel Event Policy of the Joint Commission on Accreditation of Healthcare Organizations requires a root cause analysis of a sentinel event but not a "near miss." However, root cause analysis is an important process for preventing another near miss from the same or similar cause. This article reviews the background of basic root cause analysis and provides an example of the process. Changes in policy and procedures for patient-controlled analgesia therapy, as a result of the root cause analysis, are described.

Causality↗